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O -Linked GlcNAc Modification of Cardiac Myofilament Proteins: A Novel Regulator of Myocardial Contractile Function
Open accessAbstract
In addition to O -phosphorylation, O -linked modifications of serine and threonine by β- N -acetyl- d -glucosamine (GlcNAc) may regulate muscle contractile function. This study assessed the potential role of O -GlcNAcylation in cardiac muscle contractile activation. To identify specific sites of O -GlcNAcylation in cardiac myofilament proteins, a recently developed methodology based on GalNAz-biotin labeling followed by dithiothreitol replacement and light chromatography/tandem mass spectrometry site mapping was adopted. Thirty-two O -GlcNAcylated peptides from cardiac myofilaments were identified on cardiac myosin heavy chain, actin, myosin light chains, and troponin I. To assess the potential physiological role of the GlcNAc, force–[Ca 2+ ] relationships were studied in skinned rat trabeculae. Exposure to GlcNAc significantly decreased calcium sensitivity (pCa50), whereas maximal force ( F max ) and Hill coefficient ( n ) were not modified. Using a pan-specific O -GlcNAc antibody, it was determined that acute exposure of myofilaments to GlcNAc induced a significant increase in actin O -GlcNAcylation. This study provides the first identification of O -GlcNAcylation sites in cardiac myofilament proteins and demonstrates their potential role in regulating myocardial contractile function.
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“The PTMs of sarcomeric proteins are known to be important mediators of cardiac signaling and exert various effects on contractile function (13–17).”
From Distinct hypertrophic cardiomyopathy genotypes result in convergent sarcomeric proteoform profiles revealed by top-down proteomics (2020) · cites this paper as [17]
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Diabetic Cardiomyopathy Revisited
Boudina, S., & Abel, E. D. (2007) · Circulation · doi:10.1161/circulationaha.106.679597 · Cited by 1,543
Cycling of O-linked β-N-acetylglucosamine on nucleocytoplasmic proteins
Hart, G. W., Housley, M. P., & Slawson, C. (2007) · Nature · doi:10.1038/nature05815 · Cited by 1,418
Self-citationWang, Z., Guček, M., & Hart, G. W. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0806216105 · Cited by 341
Self-citationCharacterization of a Mouse Monoclonal Antibody Specific for O-Linked N-Acetylglucosamine
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Self-citationMechanisms of [Ca2+]i Transient Decrease in Cardiomyopathy of db/db Type 2 Diabetic Mice
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Cardioprotection by N -Acetylglucosamine Linkage to Cellular Proteins
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Self-citationHu, Y., Belke, D. D., Suárez, J., Swanson, E., Clark, R., Hoshijima, M., & DILLMANN, W. H. (2005) · Circulation Research · doi:10.1161/01.res.0000165478.06813.58 · Cited by 228
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Hart, G. W., Slawson, C., Ramirez-Correa, G., & Lagerlof, O. (2011) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060608-102511 · Cited by 1,317
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Role of O -linked N -acetylglucosamine protein modification in cellular (patho)physiology
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Metadata
- Authors
- Ramirez-Correa, G. A., Jin, W., Wang, Z., Zhong, X., Gao, W. D., Dias, W. B., Vecoli, C., Hart, G. W., & Murphy, A. M.
- Affiliations
- From the Departments of Pediatrics (G.A.R.-C., W.J., A.M.M.) and Medicine (C.V.), Division of Cardiology; Departments of Biological Chemistry (Z.W., W.B.D., G.W.H.) and Anesthesia and Critical Care Medicine (X.Z., W.D.G.), Johns Hopkins University School of Medicine, Baltimore, Md; and Department of Pathophysiology (X.Z.), Harbin Medical University, People’s Republic of China.
- Johns Hopkins University (US)
- Venue
- Circulation Research
- Published
- 2008-12-05
- Volume
- 103
- Issue
- 12
- Pages
- 1354-1358
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- PMC
- PMC2615199
- OpenAlex
- W2120416709
- Cited by
- 145
- References
- 25
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 12 · 2023: 7 · 2022: 7 · 2021: 8 · 2020: 15 · 2019: 4 · 2018: 12 · 2017: 3 · 2016: 12 · 2015: 7 · 2014: 13 · 2013: 5 · 2012: 14
- FWCI
- 2.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (n01hv28180-13-0-0)
- National Heart, Lung, and Blood Institute (N01 HV-28180, R01HL063038, N01 HV028180, R01-HL-63038)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK061671, R01-DK61671)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- O-GlcNAcylation, cardiac contractility, calcium sensitivity, myosin heavy chain, myosin light chain, troponin I, Hill coefficient
- MeSH terms
- Acetylglucosamine, Acylation, Animals, Carbohydrate Conformation, Mice, Inbred C57BL, Actin Cytoskeleton, Myocardial Contraction, Myocardium, Mice, Rats
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