The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Optimizing Engineered Heart Tissue for Therapeutic Applications as Surrogate Heart Muscle
Open accessAbstract
Background— Cardiac tissue engineering aims at providing heart muscle for cardiac regeneration. Here, we hypothesized that engineered heart tissue (EHT) can be improved by using mixed heart cell populations, culture in defined serum-free and Matrigel-free conditions, and fusion of single-unit EHTs to multi-unit heart muscle surrogates. Methods and Results— EHTs were constructed from native and cardiac myocyte enriched heart cell populations. The former demonstrated a superior contractile performance and developed vascular structures. Peptide growth factor-supplemented culture medium was developed to maintain contractile EHTs in a serum-free environment. Addition of triiodothyronine and insulin facilitated withdrawal of Matrigel from the EHT reconstitution mixture. Single-unit EHTs could be fused to form large multi-unit EHTs with variable geometries. Conclusions— Simulating a native heart cell environment in EHTs leads to improved function and formation of primitive capillaries. The latter may constitute a preformed vascular bed in vitro and facilitate engraftment in vivo. Serum- and Matrigel-free culture conditions are expected to reduce immunogenicity of EHT. Fusion of single-unit EHT allows production of large heart muscle constructs that may eventually serve as optimized tissue grafts in cardiac regeneration in vivo.
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Tissue Engineering of a Differentiated Cardiac Muscle Construct
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Self-citationRadisic, M., Park, H., Shing, H., Consi, T., Schoen, F. J., Langer, R., Freed, L. E., & Vunjak-Novakovic, G. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407817101 · Cited by 910
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Unchain my heart: the scientific foundations of cardiac repair
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Self-citationBioengineered Cardiac Grafts : A New Approach to Repair the Infarcted Myocardium?
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Survival and Function of Bioengineered Cardiac Grafts
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Cell-Based Cardiac Repair: Reflections at the 10-Year Point
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Modular tissue engineering: engineering biological tissues from the bottom up
Nichol, J. W., & Khademhosseini, A. (2009) · Soft Matter · doi:10.1039/b814285h · Cited by 567
Development of a drug screening platform based on engineered heart tissue
Hansen, A., Eder, A., Bönstrup, M., Flato, M., Mewe, M., Schaaf, S., Aksehirlioglu, B., Schwörer, A., Uebeler, J., & Eschenhagen, T. (2010) · Circulation Research · doi:10.1161/circresaha.109.211458 · Cited by 510
Boudou, T., Legant, W. R., Mu, A., Borochin, M. A., Thavandiran, N., Radisic, M., Zandstra, P. W., Epstein, J. A., Margulies, K. B., & Chen, C. S. (2012) · Tissue Engineering Part A · doi:10.1089/ten.tea.2011.0341 · Cited by 413
Cardiac Tissue Engineering: State of the Art
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Self-citationHuman Engineered Heart Tissue: Analysis of Contractile Force
Mannhardt, I., Breckwoldt, K., Letuffe-Brenière, D., Schaaf, S., Schulz, H., Neuber, C., Benzin, A., Werner, T., Eder, A., Schulze, T., et al. (2016) · Stem Cell Reports · doi:10.1016/j.stemcr.2016.04.011 · Cited by 398
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Metadata
- Authors
- Naito, H., Melnychenko, I., Didié, M., Schneiderbanger, K., Schubert, P., Rosenkranz, S., Eschenhagen, T., & Zimmermann, W.-H.
- Venue
- Circulation
- Published
- 2006-07-04
- Volume
- 114
- Issue
- 1_supplement
- Pages
- I72-8
- ISSN
- 0009-7322 (ISSN-L)
- 1524-4539
- OpenAlex
- W2018463503
- Cited by
- 301
- References
- 20
- FWCI
- 18.08
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Naito, H., Melnychenko, I., Didié, M., Schneiderbanger, K., Schubert, P., Rosenkranz, S., Eschenhagen, T., & Zimmermann, W.-H. (2006). Optimizing Engineered Heart Tissue for Therapeutic Applications as Surrogate Heart Muscle. Circulation. https://doi.org/10.1161/circulationaha.105.001560
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