Electrospinning and Evaluation of PHBV-Based Tissue Engineering Scaffolds with Different Fibre Diameters, Surface Topography and Compositions
Abstract
While electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA…
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Due to its high crystallinity, PHBV is stiff and brittle, resulting in very poor mechanical properties.
“Due to its high crystallinity, PHBV is stiff and brittle, resulting in very poor mechanical properties.”
From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [101]
PHBV is unstable at a temperature close to its melting point, resulting in a significant decrease in molecular weight during its processing.
“In addition, PHBV is unstable at a temperature close to its melting point, resulting in a significant decrease in molecular weight during its processing [101].”
From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [101]
PHBV has a high melting point and high crystallization.
“PHBV has a high melting point and high crystallization.”
From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [101]
Cites
Keun Kwon, I., Kidoaki, S., & Matsuda, T. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2004.10.007 · Cited by 607 · see citing works
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Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review
Vach Agocsova, S., Culenova, M., Birova, I., Omanikova, L., Moncmanova, B., Danisovic, L., Ziaran, S., Bakos, D., & Alexy, P. (2023) · Materials · doi:10.3390/ma16124267 · Cited by 98 · see citing works
- Authors
- Tong, H.-W., Wang, M., & Lu, W. W.
- Affiliations
- University of Hong Kong (HK)
- Venue
- Journal of Biomaterials Science, Polymer Edition
- Publisher
- Informa UK Limited
- Published
- 2012-01-01
- Volume
- 23
- Issue
- 6
- Pages
- 779-806
- ISSN
- 0920-5063 (ISSN-L)
- 1568-5624
- OpenAlex
- W2052052477
- Cited by
- 34
- References
- 1
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2019: 9 · 2017: 4 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomaterials Science Polymer Edition (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Keywords
- poly(3-hydroxybutyrate-co-3-hydroxyvalerate), electrospun scaffolds, fiber diameter, surface topography, nanocomposite fibres, wettability, tensile properties, Saos-2 cells, osteoblast-like cells, cell proliferation, alkaline phosphatase activity, tissue engineering scaffolds
- MeSH terms
- Polyhydroxybutyrates, Alkaline Phosphatase, Cell Adhesion, Cell Line, Cell Movement, Humans, Materials Testing, Osteoblasts, Polyesters, Salts, Solvents, Surface Properties, Tensile Strength, Water, Porosity, Durapatite, Bone Substitutes, Tissue Engineering, Cell Proliferation, Nanoparticles, Tissue Scaffolds
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