Cytochrome Oxidase Activity and Mitochondrial Gene Expression in Skeletal Muscle of Patients with Chronic Obstructive Pulmonary Disease
Abstract
Several recent studies have suggested that skeletal muscle bioenergetics are abnormal in patients with chronic obstructive pulmonary disease (COPD). This study investigates the activity of cytochrome oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain, and the expression of two mitochondrial DNA genes related to COX (mRNA of subunit I of COX [COX-I] and the RNA component of the 12S ribosomal subunit [12S rRNA]), in quadriceps femoris muscle biopsies obtained from COPD patients with various degrees of arterial hypoxemia, and from healthy sedentary control subjects of similar age. The activity of COX was measured spectrophotometrically in fresh tissue at 37 degrees C with excess substrate. RNA transcripts were measured using reverse transcription and polymerase chain reaction. The measurements of mRNA COX-I and 12S rRNA were normalized to the mRNA of actin, which is a housekeeping gene not influenced by hypoxia. We found that, compared with control subjects, COPD patients with chronic respiratory failure (PaO2 < 60 mm Hg) showed increased COX activity (p < 0.05). Further, the activity of COX was inversely related to arterial PO2 value (Rho -0.59, p < 0.01). The COX-I mRNA content was not different between patients and control subjects but patients with chronic respiratory failure had higher levels of 12S rRNA (p < 0.05), which were again inversely related to PaO2 (Rho -0.49, p < 0.05). These results indicate that the activity of COX is increased in…
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Some authors have found increased COX activity in COPD patients with resting hypoxemia and with exercise hypoxemia, while others have not found any significant increase.
“While some authors have found an increased COX activity in COPD patients with resting hypoxemia [96] and with exercise hypoxemia [97], others have not found any significant increase [98].”
From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [96]
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Self-citationMain Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting
Henrot, P., Dupin, I., Schilfarth, P., Esteves, P., Blervaque, L., Zysman, M., Gouzi, F., Hayot, M., Pomiès, P., & Berger, P. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24076454 · Cited by 85 · see citing works
“While some authors have found an increased COX activity in COPD patients with resting hypoxemia [96] and with exercise hypoxemia [97], others have not found any significant increase [98].”
Background
- Authors
- SAULEDA, J., GARCÍA-PALMER, F., WIESNER, R. J., TARRAGA, S., HARTING, I., TOMÁS, P., GÓMEZ, C., SAUS, C., PALOU, A., & AGUSTÍ, A. G. N.
- Affiliations
- Heidelberg University (DE)
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-05-01
- Volume
- 157
- Issue
- 5
- Pages
- 1413-1417
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- OpenAlex
- W2066569687
- Cited by
- 154
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 2 · 2016: 6 · 2015: 6 · 2014: 5 · 2013: 4 · 2012: 8
- FWCI
- 2.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Respiratory and Critical Care Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Keywords
- chronic obstructive pulmonary disease, cytochrome oxidase activity, mitochondrial electron transport chain, skeletal muscle bioenergetics, 12S rRNA, arterial hypoxemia, chronic respiratory failure, quadriceps, mitochondrial ribosome, translational regulation
- MeSH terms
- Electron Transport Complex IV, DNA, Mitochondrial, Humans, Lung Diseases, Obstructive, Male, Middle Aged, Mitochondria, Muscle, RNA, Messenger, RNA, Ribosomal, Spectrophotometry, Gene Expression, Polymerase Chain Reaction, Muscle, Skeletal
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