The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Airway Smooth Muscle Cell Proliferation Is Increased in Asthma
Abstract
UNLABELLED: Increased airway smooth muscle (ASM) within the bronchial wall of asthmatic patients has been well documented and is likely to be the result of increased muscle proliferation. We have for the first time been able to culture ASM cells from asthmatic patients and to compare their proliferation rate with that of nonasthmatic patients. Asthmatic ASM cell cultures (n = 12) were established from explanted lungs and endobronchial biopsies. Nonasthmatic ASM cells (n = 10) were obtained from explanted tissue from patients with no airway disease, emphysema, carcinoma, and fibrosing alveolitis. Cell counts, tritiated thymidine incorporation, and cell cycle analysis were conducted over 7 d. Asthmatic ASM cell numbers at Days 3, 5, and 7 were significantly higher than corresponding values for nonasthmatic cells (p < 0.05). Tritiated thymidine incorporation was increased 3.2-fold in asthmatic cells compared with nonasthmatic cells within the first 24 h (p = 0.026). Flow cytometric analysis of DNA content on Days 1 and 2 revealed that a significantly greater percentage of asthmatic ASM cells were in the G2 + M phase (p < 0.05). This study shows for the first time that proliferation of ASM cells is increased in patients with asthma and provides evidence for an intrinsic abnormality in the ASM cell in this disease. KEYWORDS: asthma; human airway smooth muscle; cell culture; cell proliferation; hyperplasia
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Structure of Large and Small Airways in Nonfatal and Fatal Asthma
Carroll, N., Elliot, J., Morton, A., & James, A. (1993) · American Review of Respiratory Disease · doi:10.1164/ajrccm/147.2.405 · Cited by 720
Dunnill, M. S., Massarella, G. R., & Anderson, J. (1969) · Thorax · doi:10.1136/thx.24.2.176 · Cited by 697
Ebina, M., Takahashi, T., Chiba, T., & Motomiya, M. (1993) · American Review of Respiratory Disease · doi:10.1164/ajrccm/148.3.720 · Cited by 607
Cristofalo, V. J., Allen, R. G., Pignolo, R. J., Martin, B. G., & Beck, J. C. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.18.10614 · Cited by 556
Heparin and PGE2 inhibit DNA synthesis in human airway smooth muscle cells in culture
Johnson, P. R. A., Armour, C. L., Carey, D., & Black, J. L. (1995) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.1995.269.4.l514 · Cited by 101
Self-citationBronchoalveolar Lavage Fluid from Asthmatic Subjects Is Mitogenic for Human Airway Smooth Muscle
Naureckas, E. T., Ndukwu, I. M., Halayko, A. J., MAXWELL, C., Hershenson, M. B., & Solway, J. (1999) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.160.6.9903131 · Cited by 90
Interleukin-4 inhibits mitogen-induced proliferation of human airway smooth muscle cells in culture
Hawker, K. M., Johnson, P. R. A., Hughes, J. M., & Black, J. L. (1998) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.1998.275.3.l469 · Cited by 79
Self-citationWhat Determines Asthma Phenotype? Is It the Interaction between Allergy and the Smooth Muscle?
BLACK, J. L., & Johnson, P. R. A. (2000) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.161.supplement_2.a1q4-12 · Cited by 23
Self-citation
Cited by
Airway Structural Alterations Selectively Associated with Severe Asthma
Benayoun, L., Druilhe, A., Dombret, M.-C., Aubier, M., & Pretolani, M. (2003) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200209-1030oc · Cited by 800
Airway remodeling in asthma: New insights
Nelson, H. S., Davies, D. E., Wicks, J., Powell, R. M., Puddicombe, S. M., & Holgate, S. T. (2003) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2003.128 · Cited by 488
Mechanisms of airway hyperresponsiveness
COCKCROFT, D., & DAVIS, B. (2006) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2006.07.012 · Cited by 411
Inhibition of airway remodeling in IL-5–deficient mice
Cho, J. Y., Miller, M., Baek, K. J., Han, J. W., Nayar, J., Lee, S. Y., McElwain, K., McElwain, S., Friedman, S., & Broide, D. H. (2004) · Journal of Clinical Investigation · doi:10.1172/jci19133 · Cited by 377
Remodeling and Inflammation of Bronchi in Asthma and Chronic Obstructive Pulmonary Disease
Jeffery, P. K. (2004) · Proceedings of the American Thoracic Society · doi:10.1513/pats.200402-009ms · Cited by 347
Metadata
- Authors
- JOHNSON, P. R. A., ROTH, M., TAMM, M., HUGHES, M., GE, Q., KING, G., BURGESS, J. K., & BLACK, J. L.
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-08-01
- Volume
- 164
- Issue
- 3
- Pages
- 474-477
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- OpenAlex
- W2118304778
- Cited by
- 517
- References
- 8
- FWCI
- 11.97
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Airway Smooth Muscle Cell Proliferation Is Increased in Asthma. https://app.cochat.ai/papers/10.1164/ajrccm.164.3.2010109
- The paper
- JOHNSON, P. R. A., ROTH, M., TAMM, M., HUGHES, M., GE, Q., KING, G., BURGESS, J. K., & BLACK, J. L. (2001). Airway Smooth Muscle Cell Proliferation Is Increased in Asthma. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/ajrccm.164.3.2010109
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
