The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Mechanical Ventilation–induced Diaphragm Disuse in Humans Triggers Autophagy
Open accessAbstract
RATIONALE: Controlled mechanical ventilation (CMV) results in atrophy of the human diaphragm. The autophagy-lysosome pathway (ALP) contributes to skeletal muscle proteolysis, but its contribution to diaphragmatic protein degradation in mechanically ventilated patients is unknown. OBJECTIVES: To evaluate the autophagy pathway responses to CMV in the diaphragm and limb muscles of humans and to identify the roles of FOXO transcription factors in these responses. METHODS: Muscle biopsies were obtained from nine control subjects and nine brain-dead organ donors. Subjects were mechanically ventilated for 2 to 4 hours and 15 to 276 hours, respectively. Activation of the ubiquitin-proteasome system was detected by measuring mRNA expressions of Atrogin-1, MURF1, and protein expressions of UBC2, UBC4, and the α subunits of the 20S proteasome (MCP231). Activation of the ALP was detected by electron microscopy and by measuring the expressions of several autophagy-related genes. Total carbonyl content and HNE-protein adduct formation were measured to assess oxidative stress. Total AKT, phosphorylated and total FOXO1, and FOXO3A protein levels were also measured. MEASUREMENTS AND MAIN RESULTS: Prolonged CMV triggered activation of the ALP as measured by the appearance of autophagosomes in the diaphragm and increased expressions of autophagy-related genes, as compared with controls. Induction of autophagy was associated with increased protein oxidation and enhanced expression of the FOXO1 gene, but not the FOXO3A gene. CMV also triggered the inhibition of both AKT expression and FOXO1 phosphorylation. CONCLUSIONS: We propose that prolonged CMV causes diaphragm disuse, which, in turn, leads to activation of the ALP through oxidative stress and the induction of the FOXO1 transcription factor.
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Cites
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Regulation mechanisms and signaling pathways of autophagy
He, C., & Klionsky, D. J. (2009) · Annual Review of Genetics · doi:10.1146/annurev-genet-102808-114910 · Cited by 3,609
Sandri, M., Sandri, C., Gilbert, A., Skurk, C., Calabria, E., Picard, A., Walsh, K., Schiaffino, S., Lecker, S. H., & Goldberg, A. L. (2004) · Cell · doi:10.1016/s0092-8674(04)00400-3 · Cited by 3,012
Bodine, S. C., Stitt, T. N., Gonzalez, M., Kline, W. O., Stover, G. L., Bauerlein, R., Zlotchenko, E., Scrimgeour, A., Lawrence, J. C., Glass, D. J., et al. (2001) · Nature Cell Biology · doi:10.1038/ncb1101-1014 · Cited by 2,752
FoxO3 Controls Autophagy in Skeletal Muscle In Vivo
Mammucari, C., Milan, G., Romanello, V., Masiero, E., Rudolf, R., Del Piccolo, P., Burden, S. J., Di Lisi, R., Sandri, C., Zhao, J., et al. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.11.001 · Cited by 1,953
Rapid Disuse Atrophy of Diaphragm Fibers in Mechanically Ventilated Humans
Levine, S., Nguyen, T., Taylor, N., Friscia, M. E., Budak, M. T., Rothenberg, P., Zhu, J., Sachdeva, R., Sonnad, S., Kaiser, L. R., et al. (2008) · New England Journal of Medicine · doi:10.1056/nejmoa070447 · Cited by 1,577
Multiple types of skeletal muscle atrophy involve a common program of changes in gene expression
Lecker, S. H., Jagoe, R. T., Gilbert, A., Gomes, M., Baracos, V., Bailey, J., Price, S. R., Mitch, W. E., & Goldberg, A. L. (2004) · The FASEB Journal · doi:10.1096/fj.03-0610com · Cited by 1,539 · cited 1×
“Lecker and colleagues (52) have reported that FOXO1 gene expression is substantially induced in atrophied muscles of fasted mice, rats with cancer cachexia, streptozotocin-induced diabetes mellitus, and uremia.”
BackgroundZhao, J., Brault, J. J., Schild, A., Cao, P., Sandri, M., Schiaffino, S., Lecker, S. H., & Goldberg, A. L. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.11.004 · Cited by 1,489
Percutaneous Needle Biopsy of Skeletal Muscle in Physiological and Clinical Research
Bergström, J. (1975) · Scandinavian Journal of Clinical & Laboratory Investigation · doi:10.1080/00365517509095787 · Cited by 1,170
Sandri, M., Lin, J., Handschin, C., Yang, W., Arany, Z. P., Lecker, S. H., Goldberg, A. L., & Spiegelman, B. M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0607795103 · Cited by 974
Cited by
Mitochondrial quality control and muscle mass maintenance.
Romanello, V., & Sandri, M. (2016) · Frontiers in Physiology · doi:10.3389/fphys.2015.00422 · Cited by 434
Diaphragm ultrasonography to estimate the work of breathing during non-invasive ventilation
Vivier, E., Mekontso Dessap, A., Dimassi, S., Vargas, F., Lyazidi, A., Thille, A. W., & Brochard, L. (2012) · Intensive Care Medicine · doi:10.1007/s00134-012-2547-7 · Cited by 406
The Sick and the Weak: Neuropathies/Myopathies in the Critically Ill
Friedrich, O., Reid, M. B., Van den Berghe, G., Vanhorebeek, I., Hermans, G., Rich, M. M., & Larsson, L. (2015) · Physiological Reviews · doi:10.1152/physrev.00028.2014 · Cited by 396
Martin, A. D., Smith, B. K., Davenport, P. D., Harman, E., Gonzalez-Rothi, R. J., Baz, M., Layon, A. J., Banner, M. J., Caruso, L. J., Deoghare, H., et al. (2011) · Critical Care · doi:10.1186/cc10081 · Cited by 294
Metadata
- Authors
- Hussain, S. N. A., Mofarrahi, M., Sigala, I., Kim, H. C., Vassilakopoulos, T., Maltais, F., Bellenis, I., Chaturvedi, R., Gottfried, S. B., Metrakos, P., Danialou, G., Matecki, S., Jaber, S., Petrof, B. J., & Goldberg, P.
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-12-01
- Volume
- 182
- Issue
- 11
- Pages
- 1377-1386
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- OpenAlex
- W2139776775
- Cited by
- 264
- References
- 52
- FWCI
- 8.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Hussain, S. N. A., Mofarrahi, M., Sigala, I., Kim, H. C., Vassilakopoulos, T., Maltais, F., Bellenis, I., Chaturvedi, R., Gottfried, S. B., Metrakos, P., Danialou, G., Matecki, S., Jaber, S., Petrof, B. J., & Goldberg, P. (2010). Mechanical Ventilation–induced Diaphragm Disuse in Humans Triggers Autophagy. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/rccm.201002-0234oc
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