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Outgrowth of the Bacterial Airway Microbiome after Rhinovirus Exacerbation of Chronic Obstructive Pulmonary Disease
Open accessAbstract
RATIONALE: Rhinovirus infection is followed by significantly increased frequencies of positive, potentially pathogenic sputum cultures in chronic obstructive pulmonary disease (COPD). However, it remains unclear whether these represent de novo infections or an increased load of organisms from the complex microbial communities (microbiome) in the lower airways. OBJECTIVES: To investigate the effect of rhinovirus infection on the airway bacterial microbiome. METHODS: Subjects with COPD (n = 14) and healthy control subjects with normal lung function (n = 17) were infected with rhinovirus. Induced sputum was collected at baseline before rhinovirus inoculation and again on Days 5, 15, and 42 after rhinovirus infection and DNA was extracted. The V3-V5 region of the bacterial 16S ribosomal RNA gene was amplified and pyrosequenced, resulting in 370,849 high-quality reads from 112 of the possible 124 time points. MEASUREMENTS AND MAIN RESULTS: At 15 days after rhinovirus infection, there was a sixfold increase in 16S copy number (P = 0.007) and a 16% rise in numbers of proteobacterial sequences, most notably in potentially pathogenic Haemophilus influenzae (P = 2.7 × 10(-20)), from a preexisting community. These changes occurred only in the sputum microbiome of subjects with COPD and were still evident 42 days after infection. This was in contrast to the temporal stability demonstrated in the microbiome of healthy smokers and nonsmokers. CONCLUSIONS: After rhinovirus infection, there is a rise in bacterial burden and a significant outgrowth of Haemophilus influenzae from the existing microbiota of subjects with COPD. This is not observed in healthy individuals. Our findings suggest that rhinovirus infection in COPD alters the respiratory microbiome and may precipitate secondary bacterial infections.
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“The researchers observed that the growth of bacteria seemed to arise from the existing community.”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [124]
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“In subjects with chronic obstructive pulmonary disease (COPD) after hRV infection but not in healthy individuals, there was an increase in bacterial burden and growth of bacteria present at baseline, particularly H. influenzae (124).”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [124]
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“In exacerbation states, membership of the lung bacterial community shifts, often towards enrichment of the Proteobacteria phylum”
From The microbiome and critical illness (2016) · cites this paper as [146]
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Research works printed but not linked: 1
- Molyneaux, 2011, Investigating the role of rhinovirus infection in precipitating bacterial infections in COPD using culture independent molecular microbiology [abstract], Am J Respir Crit Care Med, 183, A3121
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The Microbiome and the Respiratory Tract
Dickson, R. P., Erb-Downward, J. R., Martinez, F. J., & Huffnagle, G. B. (2016) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021115-105238 · Cited by 953
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Enaud, R., Prével, R., Ciarlo, E., Beaufils, F., Wieërs, G., Guery, B. P., & Delhaès, L. (2020) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.00009 · Cited by 786
The Gut–Lung Axis in Respiratory Disease
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The role of bacteria in the pathogenesis and progression of idiopathic pulmonary fibrosis
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Self-citation
Metadata
- Authors
- Molyneaux, P. L., Mallia, P., Cox, M. J., Footitt, J., Willis-Owen, S. A. G., Homola, D., Trujillo-Torralbo, M.-B., Elkin, S., Kon, O. M., Cookson, W. O. C., Moffatt, M. F., & Johnston, S. L.
- Affiliations
- Imperial College London (GB)
- Imperial College Healthcare NHS Trust (GB)
- National Heart and Lung Institute, Imperial College, London, United Kingdom
- Corresponding author
- Moffatt, Miriam F.
- ORCID
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-11
- Volume
- 188
- Issue
- 10
- Pages
- 1224-1231
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- PMC
- PMC3863728
- OpenAlex
- W2101600300
- Cited by
- 390
- References
- 44
- Citations by year
- 2026: 10 · 2025: 20 · 2024: 26 · 2023: 18 · 2022: 32 · 2021: 30 · 2020: 34 · 2019: 33 · 2018: 35 · 2017: 43 · 2016: 33 · 2015: 34 · 2014: 40 · 2013: 2
- FWCI
- 29.53
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- American Journal of Respiratory and Critical Care Medicine (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
- Funders
- Wellcome Trust (083567/Z/07/Z, 097117, 096964/Z/11/Z)
- National Institute for Health and Care Research (CL-2008-21-014, NF-SI-0512-10126)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Asthma and respiratory diseases
- Keywords
- chronic obstructive pulmonary disease, rhinovirus infection, sputum microbiota, Haemophilus influenzae, Proteobacteria, secondary bacterial infection, respiratory viral infections, microbiome stability
- MeSH terms
- Aged, DNA, Bacterial, Female, Genetic Markers, Humans, Male, Middle Aged, Phylogeny, Picornaviridae Infections, Prospective Studies, Rhinovirus, RNA, Ribosomal, 16S, Sputum, Case-Control Studies, Sequence Analysis, DNA, Disease Progression, Pulmonary Disease, Chronic Obstructive, Microbiota
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