Effect of Concentrated Ambient Particles on Macrophage Phagocytosis and Killing of Streptococcus pneumoniae
Open accessAbstract
Particulate air pollution is linked to increased pneumonia epidemiologically and diminished lung bacterial clearance experimentally. We investigated the effect of concentrated ambient particles (CAPs, ≤ PM2.5) on the interaction of murine primary alveolar macrophages (AMs) and the murine macrophage cell line, J774 A.1, with Streptococcus pneumoniae. We found that CAPs increased binding of bacteria by both primary AMs and J774 cells (66.7 ± 10.6% and 58.9 ± 4.0%, respectively, n = 4). In contrast to bacterial binding, CAPs decreased internalization in both AMs and J774 (55.4 ± 8.5% and 54.7 ± 5.1%, respectively, n = 4). The rate of killing of internalized bacteria was similar, but CAPs caused a decrease in the absolute number of bacteria killed by macrophages, mainly due to decreased internalization. Additional analyses showed that soluble components of CAPs mediated the enhanced binding and decreased internalization of S. pneumoniae. Chelation of iron in soluble CAPs substantially reversed, while addition of iron as ferric ammonium citrate restored inhibition of phagocytosis of S. pneumoniae in vitro. The results identify phagocytic internalization as a specific target for toxic effects of air pollution particles on AMs.
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The relationship between kerosene use and tuberculosis is consistent with Pokhrel et al, Patel et al and Elf et al, who find increased tuberculosis risk for those who use kerosene as a lighting source.
“This relationship is consistent with Pokhrel et al, Patel et al and Elf et al who find increased TB risk for those who use kerosene as a lighting source.15 17 20”
From Household air pollution exposure and risk of tuberculosis: a case–control study of women in Lilongwe, Malawi (2024) · cites this paper as [15]
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SOLUBLE TRANSITION METALS MEDIATE RESIDUAL OIL FLY ASH INDUCED ACUTE LUNG INJURY
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“Particles containing easily solubilized metals cause a more rapid onset and severity of acute lung injury (42).”
BackgroundXiao, G. G., Wang, M., Li, N., Loo, J. A., & Nel, A. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m306423200 · Cited by 428 · see citing works
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Smith, K. R., McCracken, J. P., Weber, M. W., Hubbard, A., Jenny, A., Thompson, L. M., Balmes, J., Diaz, A., Arana, B., & Bruce, N. (2011) · The Lancet · doi:10.1016/s0140-6736(11)60921-5 · Cited by 560 · see citing works
Respiratory health effects of air pollution: Update on biomass smoke and traffic pollution
Laumbach, R. J., & Kipen, H. M. (2012) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2011.11.021 · Cited by 474 · see citing works
Household Air Pollution in Low- and Middle-Income Countries: Health Risks and Research Priorities
Martin, W. J., Glass, R. I., Araj, H., Balbus, J., Collins, F. S., Curtis, S., Diette, G. B., Elwood, W. N., Falk, H., Hibberd, P. L., et al. (2013) · PLoS Medicine · doi:10.1371/journal.pmed.1001455 · Cited by 83 · see citing works
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- Authors
- Zhou, H., & Kobzik, L.
- Affiliations
- Brigham and Women's Hospital (US)
- Venue
- American Journal of Respiratory Cell and Molecular Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-04-01
- Volume
- 36
- Issue
- 4
- Pages
- 460-465
- ISSN
- 1044-1549 (ISSN-L)
- 1535-4989
- PMC
- PMC1899326
- OpenAlex
- W1963975930
- Cited by
- 129
- References
- 2
- Citations by year
- 2026: 7 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 14 · 2021: 5 · 2020: 14 · 2019: 5 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 11 · 2013: 6 · 2012: 5
- FWCI
- 3.05
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal of Respiratory Cell and Molecular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (00002, HL007118)
- National Heart, Lung, and Blood Institute (T32HL007118)
- National Institute of Environmental Health Sciences (R01ES011008, ES011008 01190300002)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Indoor Air Quality and Microbial Exposure
- Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
- Keywords
- PM2.5, air pollution, alveolar macrophages, Streptococcus pneumoniae, phagocytosis, bacterial internalization, bacterial killing, iron chelation, pneumonia
- MeSH terms
- Air Pollutants, Animals, Antioxidants, Cells, Cultured, Chelating Agents, Interferon-gamma, Male, Mice, Inbred BALB C, Oxidation-Reduction, Phagocytosis, Streptococcus pneumoniae, Macrophages, Alveolar, Mice, Particulate Matter
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