The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Macrophage Depletion Inhibits Experimental Choroidal Neovascularization
Abstract
OBJECTIVE: To investigate the role of macrophages in the development of laser-induced choroidal neovascularization (CNV) by selective depletion with liposomal clodronate (Cl(2)MDP-LIP). METHODS: Laser photocoagulation was used to induce CNV in wild-type C57BL/6J mice. Animals were treated with intravenous (IV) and/or subconjunctival (SC) Cl(2)MDP-LIP or PBS-LIP at the following time points: 2 days before, immediately after, 2 days before and immediately after, or 2 days after laser injury. CNV responses were compared on the basis of en masse volumetric measurements and fluorescein angiography after laser photocoagulation. Macrophages were identified by immunostaining for F4/80, and vascular endothelial growth factor (VEGF) expression was quantified by ELISA. RESULTS: Macrophages invaded the site of laser injury within 1 day of photocoagulation and peaked at 3 days. IV Cl(2)MDP-LIP significantly decreased the volume of CNV and angiographic leakage when administered 2 days before and/or immediately after laser injury, but not when administered 2 days after injury. SC Cl(2)MDP-LIP significantly decreased lesion volume when coadministered with IV PBS-LIP but not IV Cl(2)MDP-LIP. IV Cl(2)MDP-LIP was significantly more beneficial when administered 2 days before laser injury than immediately after, but combining SC Cl(2)MDP-LIP with IV treatment eliminated this difference. Reduction in CNV volume correlated with VEGF protein levels and number of infiltrating macrophages. CONCLUSIONS: Generalized macrophage depletion reduced the size and leakage of laser-induced CNV and was associated with decreased macrophage infiltration and VEGF protein. These findings define the role of the macrophage as a critical component in initiating the laser-induced CNV response.
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Cites
(1999) · Archives of Ophthalmology · doi:10.1001/archopht.117.10.1329 · Cited by 2,192
Rooijen, N. V., & Sanders, A. (1994) · Journal of Immunological Methods · doi:10.1016/0022-1759(94)90012-4 · Cited by 1,779
F4/80, a monoclonal antibody directed specifically against the mouse macrophage
Austyn, J. M., & Gordon, S. (1981) · European Journal of Immunology · doi:10.1002/eji.1830111013 · Cited by 1,579
Barleon, B., Sozzani, S., Zhou, D., Weich, H., Mantovani, A., & Marme, D. (1996) · Blood · doi:10.1182/blood.v87.8.3336.bloodjournal8783336 · Cited by 1,356
Risk factors for age-related macular degeneration
Smith, W., Assink, J., Klein, R., Mitchell, P., Klaver, C. C. W., Klein, B. E. K., Hofman, A., Jensen, S., Wang, J. J., & de Jong, P. T. V. M. (2001) · Ophthalmology · doi:10.1016/s0161-6420(00)00580-7 · Cited by 1,071
The Vascular Endothelial Growth Factor Receptor Flt-1 Mediates Biological Activities
Clauss, M., Weich, H., Breier, G., Knies, U., Röckl, W., Waltenberger, J., & Risau, W. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.30.17629 · Cited by 836
Krzystolik, M. G. (2002) · Archives of Ophthalmology · doi:10.1001/archopht.120.3.338 · Cited by 590
Blaauwgeers, H. G. T., Holtkamp, G. M., Rutten, H., Witmer, A. N., Koolwijk, P., Partanen, T. A., Alitalo, K., Kroon, M. E., Kijlstra, A., van Hinsbergh, V. W. M., et al. (1999) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)65138-3 · Cited by 520
VEGF is major stimulator in model of choroidal neovascularization.
Kwak, N., Okamoto, N., Wood, J. M., & Campochiaro, P. A. (2000) · PubMed · Cited by 442
Grossniklaus, H. E., Ling, J. X., Wallace, T. M., Dithmar, S., Lawson, D., Cohen, C., Elner, V. M., Elner, S. G., & Sternberg, P. (2002) · PubMed · Cited by 434
Cited by
Fantin, A., Vieira, J. M., Gestri, G., Denti, L., Schwarz, Q., Prykhozhij, S., Peri, F., Wilson, S. W., & Ruhrberg, C. (2010) · Blood · doi:10.1182/blood-2009-12-257832 · Cited by 1,153
The role of macrophage phenotype in vascularization of tissue engineering scaffolds
Spiller, K. L., Anfang, R. R., Spiller, K. J., Ng, J., Nakazawa, K. R., Daulton, J. W., & Vunjak-Novakovic, G. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.02.012 · Cited by 948
Drusen complement components C3a and C5a promote choroidal neovascularization
Nozaki, M., Raisler, B. J., Sakurai, E., Sarma, J. V., Barnum, S. R., Lambris, J. D., Chen, Y., Zhang, K., Ambati, B. K., Baffi, J. Z., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408835103 · Cited by 650
Self-citationAn animal model of age-related macular degeneration in senescent Ccl-2- or Ccr-2-deficient mice
Ambati, J., Anand, A., Fernandez, S., Sakurai, E., Lynn, B. C., Kuziel, W. A., Rollins, B. J., & Ambati, B. K. (2003) · Nature Medicine · doi:10.1038/nm950 · Cited by 631
Self-citationMolecular pathology of age-related macular degeneration
Ding, X., Patel, M., & Chan, C.-C. (2009) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2008.10.001 · Cited by 582
“Espinosa-Heidmann et al. (2003) and Sakurai et al. (2003) and their colleagues showed that when administered to mice, these liposomes markedly impaired macrophage recruitment to areas of laser retinal injury.”
Background
Metadata
- Authors
- Sakurai, E., Anand, A., Ambati, B. K., van Rooijen, N., & Ambati, J.
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2003-08-01
- Volume
- 44
- Issue
- 8
- Pages
- 3578
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- OpenAlex
- W2050019961
- Cited by
- 503
- References
- 53
- FWCI
- 14.70
- Type
- journal article
- Language
- en
- Open access
- closed
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