The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Roles of Thrombospondin-1 and -2 in Regulating Corneal and Iris Angiogenesis
Open accessAbstract
PURPOSE: Thrombospondin (TSP)-1 and -2 are important antiangiogenic factors thought to be involved in maintaining corneal avascularity (angiogenic privilege). This study was undertaken to investigate whether deficiencies of these factors altered developmental and inflammation-induced angiogenesis in the cornea and developmental angiogenesis of the iris of mice. METHODS: Expression of TSP-1 and -2 mRNA and protein was assayed in cornea and iris stroma by RT-PCR and Western blot. Corneas and irides of TSP-1(-/-), TSP-2(-/-), and TSP-1,2(-/-) mice aged 2, 3, and 6 months, and wild-type control mice, were analyzed for spontaneous angiogenesis biomicroscopically, histologically, and with CD31 immunohistochemistry. The mouse model of suture-induced, inflammatory corneal neovascularization was used to evaluate the lack of TSP-1,2 and both TSPs on induced-corneal angiogenesis. Seven days after intrastromal placement of three 11-0 sutures, vascularized areas were analyzed morphometrically on CD31-stained corneal flatmounts. RESULTS: Corneas and irises from normal mouse eyes constitutively expressed TSP-1 and -2 mRNAs and proteins. Corneas of TSP-1(-/-), -2(-/-), and -1,2(-/-) mice displayed no evidence of spontaneous developmental-postnatal angiogenesis, although irises of these mice contained significantly increased iris vessel density compared with wild-type animals (P < 0.01). One week after suturing, corneas of all TSP(-/-) mice had significantly greater corneal angiogenesis than those of control mice (P < 0.05). TSP-1(-/-) had a significantly greater effect on induced corneal neovascularization than did TSP-2(-/-), with the opposite being the case in developmental iris angiogenesis (P < 0.01). CONCLUSIONS: Corneal avascularity during development is redundantly regulated, shown by the fact that lack of the antiangiogenic factors TSP-1 and/or -2 resulted in no spontaneous corneal angiogenesis. By contrast, TSP-1, more than TSP-2, helps to suppress inflammation-induced corneal angiogenesis postnatally, implying that angiogenic privilege in the cornea is actively maintained.
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Citations
Cites
Angiogenesis in cancer and other diseases
Carmeliet, P., & Jain, R. K. (2000) · Nature · doi:10.1038/35025220 · Cited by 9,070
Cited by
Eghrari, A. O., Riazuddin, S. A., & Gottsch, J. D. (2015) · Progress in Molecular Biology and Translational Science · doi:10.1016/bs.pmbts.2015.04.001 · Cited by 357
Cursiefen, C., Cao, J., Chen, L., Liu, Y., Maruyama, K., Jackson, D., Kruse, F. E., Wiegand, S. J., Dana, M. R., & Streilein, J. W. (2004) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.03-1380 · Cited by 329
Self-citationBevacizumab as a Potent Inhibitor of Inflammatory Corneal Angiogenesis and Lymphangiogenesis
Bock, F., Onderka, J., Dietrich, T., Bachmann, B., Kruse, F. E., Paschke, M., Zahn, G., & Cursiefen, C. (2007) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.06-0570 · Cited by 320
Self-citationHos, D., Matthaei, M., Bock, F., Maruyama, K., Notara, M., Clahsen, T., Hou, Y., Le, V. N. H., Salabarria, A.-C., Horstmann, J., et al. (2019) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2019.07.001 · Cited by 303
Self-citationCellular and extracellular matrix modulation of corneal stromal opacity
Torricelli, A. A. M., & Wilson, S. E. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.09.013 · Cited by 124
Metadata
- Authors
- Cursiefen, C., Masli, S., Ng, T. F., Dana, M. R., Bornstein, P., Lawler, J., & Streilein, J. W.
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2004-04-01
- Volume
- 45
- Issue
- 4
- Pages
- 1117
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- OpenAlex
- W2082867958
- Cited by
- 173
- References
- 1
- FWCI
- 3.83
- Type
- journal article
- Language
- en
- Open access
- green
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