Three-Dimensional Analysis of Collagen Lamellae in the Anterior Stroma of the Human Cornea Visualized by Second Harmonic Generation Imaging Microscopy
Abstract
Purpose The structure of collagen lamellae in the anterior stroma of the human cornea is thought to be an important determinant of corneal rigidity. The three-dimensional structure of such collagen lamellae in normal human corneas was examined. Methods The anterior portion of 27 normal human corneas was obtained from donor tissue for Descemet's stripping automated endothelial keratoplasty (DSAEK) surgery, and blocks (∼3-mm square) of the central cornea were examined by second harmonic generation (SHG) imaging microscopy. Each cornea was scanned from the surface of Bowman's layer to a depth of 150 μm, and SHG forward signals were collected. The angles of collagen lamellae immediately below to a depth of 30 μm below Bowman's layer (sutural lamellae) as well as of those at a depth of 50 or 100 μm were measured. The density and width of sutural lamellae were also evaluated. Results Collagen lamellae in the anterior stroma were evenly distributed and randomly oriented. The angle of sutural lamellae relative to Bowman's layer was 19.19 ± 4.34° (mean ± SD). The angles of collagen lamellae at depths of 50 or 100 μm were 8.91 ± 2.91 and 6.91 ± 2.11°, respectively. The density of sutural lamellae was 910.0 ± 480.4/mm(2), and their width was 13.14 ± 5.03 and 7.11 ± 3.00 μm in the region immediately beneath and 30 μm below Bowman's layer, respectively. Conclusions Collagen lamellae in the anterior stroma of the normal human cornea are interwoven in three dimensions and adhere densely to…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The insertion angles of anterior lamellae into Bowman's layer are on average about 19°.
“Quantitative analysis later revealed that the insertion angles were, on average, about 19° (Morishige et al., 2011)”
From Corneal structure and transparency (2015) · cites this paper as Morishige et al., 2011
Cited by
Sorted by citations
Corneal structure and transparency
Meek, K. M., & Knupp, C. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.07.001 · Cited by 853 · see citing works
- Authors
- Morishige, N., Takagi, Y., Chikama, T.-I., Takahara, A., & Nishida, T.
- Affiliations
- Yamaguchi University (JP)
- Kyushu University (JP)
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2011-02-16
- Volume
- 52
- Issue
- 2
- Pages
- 911
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- OpenAlex
- W1988593520
- Cited by
- 80
- References
- 0
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 5 · 2017: 8 · 2016: 6 · 2015: 8 · 2014: 4 · 2013: 5 · 2012: 1
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Investigative Ophthalmology & Visual Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal surgery and disorders
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal Surgery and Treatments
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ocular Surface and Contact Lens
- Keywords
- collagen lamellae, human cornea, Bowman's layer, collagen fiber orientation, corneal rigidity, corneal curvature, second harmonic generation microscopy
- MeSH terms
- Adult, Aged, Collagen, Corneal Stroma, Female, Humans, Male, Middle Aged, Imaging, Three-Dimensional, Microscopy, Fluorescence, Multiphoton, Bowman Membrane
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
