Effects of Gold Nanoparticles on Endotoxin-Induced Uveitis in Rats
Abstract
PURPOSE: This study evaluates the effects of the gold nanoparticle in endotoxin-induced uveitis in rats. METHODS: Adult male Wistar rats were divided into five groups: saline + saline, lipopolysaccharide (LPS) + saline, LPS + prednisolone, LPS + gold salt (GS) and LPS + gold nanoparticle (GNP). Two hours after LPS administration, prednisolone acetate 1%, GS, and GNP were topically applied to both eyes of rats and repeated every 6 hours for 24 hours. After 24 hours, rats were anesthetized and aqueous humor was sampled and the irides were removed. Aqueous humor TNF-α, myeloperoxidase activity were determined. Irides oxidative damage and content of toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) were determined. RESULTS: The administration of LPS-induced eye inflammatory response characterized by an increase in aqueous humor TNF-α, myeloperoxidase, and by irides oxidative damage. All these parameters were decreased by the administration of GNP. Since the inflammatory response secondary to LPS administration depends, in part, to the activation of the TLR4-NF-κB pathway we demonstrated here that a potential mechanism to explain the GNP effects was the decrease on TLR4 content and NF-κB activation. CONCLUSIONS: These findings suggest that topical GNP decreases intraocular inflammation and oxidative damage by interfering in the TLR4-NF-κB pathway.
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In rats with endotoxin-induced uveitis, rats that received LPS only showed high expression of TNF-α, TLPR4 and NFκB, while rats that received LPS and GNP had significantly lower expression.
“The rats that received LPS only showed a high expression of TNF-α, TLPR4 and NFκB, meaning that inflammation was induced by LPS, while the rats that received LPS and GNP had a significantly lower expression.”
From Immune responses to gene editing by viral and non-viral delivery vectors used in retinal gene therapy (2022) · cites this paper as [77]
Topical GNP decreases intraocular inflammation and oxidative damage by interfering with the TLR4-NFκB pathway.
“These findings interestingly suggest that topical GNP decreases intraocular inflammation and oxidative damage by interfering with the TLR4-NFκB pathway [77].”
From Immune responses to gene editing by viral and non-viral delivery vectors used in retinal gene therapy (2022) · cites this paper as [77]
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- Authors
- Pereira, D. V., Petronilho, F., Pereira, H. R. S. B., Vuolo, F., Mina, F., Possato, J. C., Vitto, M. F., de Souza, D. R., da Silva, L., da Silva Paula, M. M., de Souza, C. T., & Dal-Pizzol, F.
- Affiliations
- Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (BR)
- Universidade do Sul de Santa Catarina (BR)
- Laboratório de Síntese de Complexos Multifuncionais, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil, and the
- Universidade do Extremo Sul Catarinense (BR)
- Laboratório de Fisiologia e Bioquímica do Exercício, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
- From the Laboratório de Fisiopatologia Experimental and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense,
- Corresponding author
- Pereira, David Valter
- Petronilho, Fabricia
- Pereira, Hilda Regina Silveira Benevides
- Vuolo, Francieli
- Mina, Francieli
- Vitto, Marcelo Fontana
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2012-12-07
- Volume
- 53
- Issue
- 13
- Pages
- 8036
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- OpenAlex
- W2334398938
- Cited by
- 76
- References
- 42
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 5 · 2023: 6 · 2022: 7 · 2021: 8 · 2020: 6 · 2019: 8 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 7 · 2014: 4 · 2013: 1
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Investigative Ophthalmology & Visual Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Ophthalmology › Ocular Diseases and Behçet’s Syndrome
- Health Sciences › Medicine › Ophthalmology › Ocular Infections and Treatments
- Health Sciences › Medicine › Ophthalmology › Glaucoma and retinal disorders
- Keywords
- gold nanoparticles, uveitis, lipopolysaccharide, endotoxin-induced inflammation, intraocular inflammation, oxidative damage, TLR4, NF-κB, TNF-α, myeloperoxidase, aqueous humor, topical ocular drug delivery, anti-inflammatory activity
- MeSH terms
- Administration, Topical, Animals, Aqueous Humor, Disease Models, Animal, Endotoxins, Enzyme-Linked Immunosorbent Assay, Iris, Lipopolysaccharides, Male, Peroxidase, Prednisolone, Tumor Necrosis Factor-alpha, Uveitis, Anterior, Blotting, Western, NF-kappa B, Rats, Wistar, Gold Compounds, Oxidative Stress, Toll-Like Receptor 4, Rats, Nanoparticles
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