Mucus clearance as a primary innate defense mechanism for mammalian airways
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Innate defenses in the lung Microanatomy of the airway surfaceWith the advent of the capacity to fix airway surface liquid (ASL) in vivo, using the perfluorocarbon/osmium technique pioneered by Sims et al. ( 12), and the development of well-differentiated (WD) human airway epithelial cultures that exhibit mucus transport in vitro (13), it is now possible to investigate the microanatomy of mucus transport on airway surfaces at high resolution.Representative images depicting the range of morphologic techniques that can be applied to this culture system are shown in Figure 2.Analysis of photomicrographs of this preparation, combined with immunocytochemical studies, have revealed several key features of the microanatomy of the ASL compartment (13,14).The ASL consists of at least two layers, a mucus layer and a periciliary liquid layer (PCL; Figure 2).The mucus layer consists of high-molecular weight, heavily glycosylated macromolecules, products of at least two distinct genes (MUC5AC and MUC5B), that behave as a tangled network of polymers Mucus clearance as a primary innate defense mechanism for mammalian airways
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Viral-mediated injury to these cells and to the epithelium lining large airways and ciliated ducts contributes to the evolution of influenza-associated bacterial pneumonia by compromising the mucus stream and hindering mucociliary clearance of bacteria from the respiratory tract.
“Viral-mediated injury to these cells and to the epithelium lining large airways and ciliated ducts undoubtedly contributes to the evolution of influenza-associated bacterial pneumonia by compromising the mucus stream and hindering mucociliary clearance of bacteria from the respiratory tract”
From Myocardial Injury and Bacterial Pneumonia Contribute to the Pathogenesis of Fatal Influenza B Virus Infection (2012) · cites this paper as [47]
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- Authors
- Knowles, M. R., & Boucher, R. C.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Boucher, Richard C.
- Venue
- Journal of Clinical Investigation
- Published
- 2002-03-01
- Volume
- 109
- Issue
- 5
- Pages
- 571-577
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W4230115907
- Cited by
- 1,054
- References
- 54
- Citations by year
- 2026: 34 · 2025: 39 · 2024: 44 · 2023: 47 · 2022: 51 · 2021: 65 · 2020: 47 · 2019: 57 · 2018: 51 · 2017: 54 · 2016: 55 · 2015: 52 · 2014: 47 · 2013: 48 · 2012: 42
- FWCI
- 23.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Respiratory and Cough-Related Research
- Keywords
- mucus clearance, innate immunity, airway surface liquid, airway epithelium, mucus layer, MUC5AC, MUC5B, mucin, mucus transport
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