The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Promotion of human adipocyte precursor replication by 17beta-estradiol in culture
Open accessAbstract
The influence of 17beta-estradiol and 17alpha-estradiol on adult human omental adipocyte precursors grown in a propagating culture system was studied. Cells were grown in subculture in the presence or absence of hormone. 17beta-estradiol resulted in significant promotion of adipocyte precursor replication, as determined by cell counting and incorporation of radioactive thymidine into DNA. The hormone stimulated cell multiplication in the concentration range 0.5--500 ng/ml growth medium. The highest level tested was 500 ng/ml. The maximal effects were obtained at 50 ng/ml (P less than 0.001 by paired t test, 48 h after hormone addition). All 10 cell strains (five were derived from men and five from women) that were tested responded similarly to the hormone. 17beta-estradiol did not affect cell size. 17alpha-estradiol did not promote the replication of adipocyte precursors, nor did it influence cell size. Thus, 17beta-estradiol, which is the active isomer in known target tissues, stimulates the multiplication of human adipocyte precursors in culture.
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“E2 also increases preadipocyte proliferation, suggesting an adipogenic effect (167).”
From Metabolic impact of endogenously produced estrogens by adipose tissue in females and males across the lifespan (2025) · cites this paper as [167]
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Cites
Female Steroid Hormones and Target Cell Nuclei
O’Malley, B. W., & Means, A. R. (1974) · Science · doi:10.1126/science.183.4125.610 · Cited by 947
Mechanism of Action of the Sex Steroid Hormones
Chan, L., & O’Malley, B. W. (1976) · New England Journal of Medicine · doi:10.1056/nejm197606102942405 · Cited by 293
Cytological and enzymological characterization of adult human adipocyte precursors in culture.
Van, R. L., Bayliss, C. E., & Roncari, D. A. K. (1976) · Journal of Clinical Investigation · doi:10.1172/jci108516 · Cited by 265
Self-citationINTERACTION OF ESTROGEN AND PROGESTERONE IN CHICK OVIDUCT DEVELOPMENT
Oka, T., & SCHIMKE, R. T. (1969) · The Journal of Cell Biology · doi:10.1083/jcb.43.1.123 · Cited by 253
The purification and properties of Neurospora malate dehydrogenase
Munkres, K. D., & Richards, F. M. (1965) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(65)90391-7 · Cited by 237
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Lindner, H. R., Perel, E., Friedlander, A. M., & Zeitlin, A. (1972) · Steroids · doi:10.1016/0039-128x(72)90076-1 · Cited by 193
Synthesis of very low density lipoproteins in the cockerel. Effects of estrogen.
Chan, L., Jackson, R. L., O’Malley, B. W., & Means, A. R. (1976) · Journal of Clinical Investigation · doi:10.1172/jci108481 · Cited by 164
Stimulation of the Synthesis of Very Low Density Lipoproteins in Rooster Liver by Estradiol
Luskey, K. L., Brown, M. S., & Goldstein, J. L. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)79909-6 · Cited by 132
Thein Vitroandin VivoUptake and Metabolism of Steroids in Human Adipose Tissue1
Bleau, G., Roberts, K. D., & Chapdelaine, A. (1974) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-39-2-236 · Cited by 82
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REVIEW ARTICLE: THE POLYCYSTIC OVARY SYNDROME
Yen, S. S. C. (1980) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1980.tb02132.x · Cited by 697
Endocrine Control of Body Composition in Infancy, Childhood, and Puberty
Veldhuis, J. D., Roemmich, J. N., Richmond, E. J., Rogol, A. D., Lovejoy, J. C., Sheffield-Moore, M., Mauras, N., & Bowers, C. Y. (2005) · Endocrine Reviews · doi:10.1210/er.2003-0038 · Cited by 452
Aromatization of Androstenedione by Human Adipose Tissue Stromal Cells in Monolayer Culture*
Ackerman, G. E., Smith, M. E., Mendelson, C. R., MacDonald, P. C., & Simpson, E. R. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-53-2-412 · Cited by 389
Role of estrogens in adipocyte development and function
Cooke, P. S., & Naaz, A. (2004) · Experimental Biology and Medicine · doi:10.1177/153537020422901107 · Cited by 370
Sex dimorphism and depot differences in adipose tissue function
White, U. A., & Tchoukalova, Y. D. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2013.05.006 · Cited by 296
Metadata
- Authors
- Roncari, D. A., & Van, R. L.
- Affiliations
- University of Toronto (CA)
- Venue
- Journal of Clinical Investigation
- Published
- 1978-09-01
- Volume
- 62
- Issue
- 3
- Pages
- 503-508
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC371792
- OpenAlex
- W2040874095
- Cited by
- 128
- References
- 18
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 5
- FWCI
- 4.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Atkinson Foundation
- Medical Research Council
- Topics
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- estradiol, 17α-estradiol, adipocyte precursors, adipocyte proliferation, cell replication, omental adipose tissue, cell size, human adipocytes
- MeSH terms
- Adipose Tissue, Adult, Cell Differentiation, Cell Division, Cells, Cultured, DNA, Estradiol, Female, Humans, Male, Thymidine
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