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Regulation by vitamin D metabolites of parathyroid hormone gene transcription in vivo in the rat.
Open accessAbstract
In vitro 1,25-dihydroxycholecalciferol (1,25(OH)2D3) decreased levels of preproparathyroid(preproPTH) hormone mRNA. We have now pursued these studies in vivo in the rat. Rats were administered vitamin D metabolites i.p. and the levels of preproPTH mRNA were determined in excised parathyroid-thyroid glands by blot hybridization. PreproPTH mRNA levels were less than 4% of basal at 48 h after 100 pmol 1,25(OH)2D3, with no increase in serum calcium. Gel blots showed that 1,25(OH)2D3 decreased preproPTH mRNA levels without any change in its size (833 basepair). Microdissected parathyroids after 1,25(OH)2D3 (100 pmol) showed mRNA levels for preproPTH were 40 +/- 8% of controls, but for beta-actin were 100% of controls. The relative potencies of vitamin D metabolites were: 1,25(OH)2D3 greater than 24,25(OH)2D3 greater than 25(OH)D3 greater than vitamin D3. In vitro nuclear transcription showed that 1,25(OH)2D3-treated (100 pmol) rats' PTH transcription was 10% of control, while beta-actin was 100%. These results show that 1,25(OH)2D3 regulates PTH gene transcription. PTH stimulates 1,25(OH)2D3 synthesis, which then inhibits PTH synthesis, thus completing an endocrinological feedback loop.
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Cited by
1,25-Dihydroxyvitamin D3 is a negative endocrine regulator of the renin-angiotensin system
Li, Y. C., Kong, J., Wei, M., Chen, Z.-F., Liu, S. Q., & Cao, L.-P. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215219 · Cited by 1,625
1,25(OH) 2 ‐Vitamin D 3 receptors: gene regulation and genetic circuitry
Minghetti, P. P., & Norman, A. W. (1988) · The FASEB Journal · doi:10.1096/fasebj.2.15.2847948 · Cited by 444
Vitamin D in chronic kidney disease: A systemic role for selective vitamin D receptor activation
Andress, D. L. (2006) · Kidney International · doi:10.1038/sj.ki.5000045 · Cited by 309
Hormones and Disorders of Mineral Metabolism
Bringhurst, F. R., Demay, M. B., & Kronenberg, H. M. (2011) · Williams Textbook of Endocrinology · doi:10.1016/b978-1-4377-0324-5.00028-6 · Cited by 298
Vitamin A antagonizes calcium response to vitamin D in man
Johansson, S., & Melhus, H. (2001) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2001.16.10.1899 · Cited by 176
Metadata
- Authors
- Silver, J., Naveh-Many, T., Mayer, H., Schmelzer, H. J., & Popovtzer, M. M.
- Venue
- Journal of Clinical Investigation
- Published
- 1986-11-01
- Volume
- 78
- Issue
- 5
- Pages
- 1296-1301
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2156887647
- Cited by
- 536
- References
- 0
- FWCI
- 13.22
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Silver, J., Naveh-Many, T., Mayer, H., Schmelzer, H. J., & Popovtzer, M. M. (1986). Regulation by vitamin D metabolites of parathyroid hormone gene transcription in vivo in the rat. Journal of Clinical Investigation. https://doi.org/10.1172/jci112714
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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