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cDNA cloning of human plasminogen activator-inhibitor from endothelial cells.
Open accessAbstract
Full-length cDNA for plasminogen activator inhibitor (PAI-1) was isolated from a human umbilical vein endothelial cell (HUVEC) lambda gt11 cDNA library. Three overlapping clones were identified by immunologic screening of 10(6) recombinant phage using a rabbit anti-human fibrosarcoma PAI-1 antiserum. The fusion proteins encoded by these three clones also react strongly with a monoclonal mouse anti-human fibrosarcoma PAI-1 antibody. By nucleotide sequence analysis, PAI-1 cDNA encodes a protein containing 402 amino acids with a predicted, nonglycosylated molecular mass of 45 kD. Identity of this material as authentic PAI-1 was confirmed by the presence of high level homology with the primary amino acid sequence of an internal peptide prepared from purified rat hepatoma PAI-1. The predicted amino acid sequence also reveals extensive homology with other members of the serine protease inhibitor gene family. Cultured HUVECs contain two PAI-1 mRNA species, both encoded by a single gene, differing by 1 kb in the 3' untranslated region. The PAI-1 gene is located on human chromosome 7.
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Cited by
Plasminogen activator inhibitors: hormonally regulated serpins
Andreasen, P. A., Georg, B., Lund, L. R., Riccio, A., & Stacey, S. N. (1990) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(90)90164-4 · Cited by 398
Self-citationPlasminogen Activator Inhibitor-1 Is a Marker and a Mediator of Senescence
Vaughan, D. E., Rai, R., Khan, S. S., Eren, M., & Ghosh, A. K. (2017) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/atvbaha.117.309451 · Cited by 258
Murano, S., Thweatt, R., Shmookler Reis, R. J., Jones, R. A., Moerman, E. J., & Goldstein, S. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.8.3905 · Cited by 199
Metadata
- Authors
- Ginsburg, D., Zeheb, R., Yang, A. Y., Rafferty, U. M., Andreasen, P. A., Nielsen, L., Dano, K., Lebo, R. V., & Gelehrter, T. D.
- Venue
- Journal of Clinical Investigation
- Published
- 1986-12-01
- Volume
- 78
- Issue
- 6
- Pages
- 1673-1680
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2049626340
- Cited by
- 346
- References
- 0
- FWCI
- 18.60
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ginsburg, D., Zeheb, R., Yang, A. Y., Rafferty, U. M., Andreasen, P. A., Nielsen, L., Dano, K., Lebo, R. V., & Gelehrter, T. D. (1986). cDNA cloning of human plasminogen activator-inhibitor from endothelial cells. Journal of Clinical Investigation. https://doi.org/10.1172/jci112761
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