The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica.
Open accessAbstract
Entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (Gal) or N-acetyl-D-galactosamine (GalNAc) inhibitable surface lectin. Blockade of this adherence lectin with Gal or GalNAc in vitro prevents amebic killing of target cells. We have identified and purified the adherence lectin by two methods: affinity columns derivatized with galactose monomers or galactose terminal glycoproteins, and affinity columns and immunoblots prepared with monoclonal antibodies that inhibit amebic adherence. By both methods the adherence lectin was identified as a 170-kD secreted and membrane-bound amebic protein. The surface location of the lectin was confirmed by indirect immunofluorescence. Purified lectin competitively inhibited amebic adherence to target cells by binding to receptors on the target Chinese hamster ovary cells in a Gal-inhibitable manner.
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Cites
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
Carbohydrate-Specific Receptors of the Liver
Ashwell, G., & Harford, J. (1982) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.51.070182.002531 · Cited by 1,914
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Petri, W. A., Haque, R., & Mann, B. J. (2002) · Annual Review of Microbiology · doi:10.1146/annurev.micro.56.012302.160959 · Cited by 326
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Metadata
- Authors
- Petri, W. A., Smith, R. D., Schlesinger, P. H., Murphy, C. F., & Ravdin, J. I.
- Venue
- Journal of Clinical Investigation
- Published
- 1987-11-01
- Volume
- 80
- Issue
- 5
- Pages
- 1238-1244
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1985689820
- Cited by
- 281
- References
- 2
- FWCI
- 9.32
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. https://app.cochat.ai/papers/10.1172/jci113198
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- Petri, W. A., Smith, R. D., Schlesinger, P. H., Murphy, C. F., & Ravdin, J. I. (1987). Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. Journal of Clinical Investigation. https://doi.org/10.1172/jci113198
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
