The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Induction of platelet-derived growth factor A-chain and c-myc gene expressions by angiotensin II in cultured rat vascular smooth muscle cells.
Open accessAbstract
Recently, angiotensin II (Ang II) has been shown to cause hypertrophy of cultured quiescent rat aortic smooth muscle (RASM) cells. This observation along with the demonstration of angiotensinogen mRNA in the vessel wall has led us to postulate a role for vascular angiotensin in hypertensive blood vessel hypertrophy. To investigate further the possible molecular mechanisms, we examined the effect of Ang II on the expression of two genes known to be involved with cellular growth response. Near-confluent RASM cells were made quiescent by 48-h exposure to a defined serum-free medium. Ang II (10(-6) to 10(-11) M) resulted in an induction of the protooncogene c-myc mRNA within 30 min which persisted for 6 h. Interestingly, 6 h after the addition of Ang II, platelet-derived growth factor (PDGF) A-chain mRNA expression was elevated, peaked in 9 h, and persisted for 11 h. This was accompanied with a 15-20-fold increase in PDGF concentration in the culture medium. These effects were dose-dependent and were blocked by saralasin. Whereas the inhibition of protein synthesis by cycloheximide resulted in a stabilization of c-myc mRNA, cycloheximide abolished the elevation of the PDGF A-chain mRNA. Taken together, our data show that exposure of RASM cells to Ang II results in the sequential activation of c-myc and PDGF A-chain mRNA expressions. This sequential activation of protooncogene and growth factor gene may be an important mechanism in angiotensin-induced smooth muscle growth and hypertrophy.
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Cites
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,887
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene
Greenberg, M. E., & Ziff, E. B. (1984) · Nature · doi:10.1038/311433a0 · Cited by 3,064
Induction of c-fos gene and protein by growth factors precedes activation of c-myc
Müller, R., Bravo, R., Burckhardt, J., & Curran, T. (1984) · Nature · doi:10.1038/312716a0 · Cited by 1,369
Angiotensin II induces hypertrophy, not hyperplasia, of cultured rat aortic smooth muscle cells.
Geisterfer, A. A., Peach, M. J., & Owens, G. K. (1988) · Circulation Research · doi:10.1161/01.res.62.4.749 · Cited by 1,065
Greenberg, M. E., Greene, L. A., & Ziff, E. B. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38689-1 · Cited by 875
Betsholtz, C., Johnsson, A., Heldin, C.-H., Westermark, B., Lind, P., Urdea, M. S., Eddy, R., Shows, T. B., Philpott, K., Mellor, A. L., et al. (1986) · Nature · doi:10.1038/320695a0 · Cited by 781
Heldin, C. H., Bäckström, G., Ostman, A., Hammacher, A., Rönnstrand, L., Rubin, K., Nistér, M., & Westermark, B. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02955.x · Cited by 498
Folkow, B., Hallbäck, M., Lundgren, Y., Sivertsson, R., & Weiss, L. (1972) · Spontaneous Hypertension · doi:10.1007/978-3-642-65441-1_23 · Cited by 497
Cultured endothelial cells produce a platelet-derived growth factor-like protein
DiCorleto, P. E., & Bowen-Pope, D. F. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.7.1919 · Cited by 497
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Cooke, M., & Dzau, M. (1997) · Annual Review of Medicine · doi:10.1146/annurev.med.48.1.489 · Cited by 710
Emerging role of angiotensin-converting enzyme inhibitors in cardiac and vascular protection.
Lonn, E. M., Yusuf, S., Jha, P., Montague, T. J., Teo, K. K., Benedict, C. R., & Pitt, B. (1994) · Circulation · doi:10.1161/01.cir.90.4.2056 · Cited by 503
Han, Y., Runge, M. S., & Brasier, A. R. (1999) · Circulation Research · doi:10.1161/01.res.84.6.695 · Cited by 377
Williams, B., Baker, A. Q., Gallacher, B., & Lodwick, D. (1995) · Hypertension · doi:10.1161/01.hyp.25.5.913 · Cited by 301
Inhibiting the Effects of Angiotensin on Cardiovascular Hypertrophy
Metsärinne, K. P., Stoll, M., Falkenhahn, M., Gohlke, P., & Unger, T. (1994) · Angiotensin Receptors · doi:10.1007/978-1-4615-2464-9_13 · Cited by 6
Metadata
- Authors
- Naftilan, A. J., Pratt, R. E., & Dzau, V. J.
- Venue
- Journal of Clinical Investigation
- Published
- 1989-04-01
- Volume
- 83
- Issue
- 4
- Pages
- 1419-1424
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1990132316
- Cited by
- 623
- References
- 32
- FWCI
- 36.41
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Naftilan, A. J., Pratt, R. E., & Dzau, V. J. (1989). Induction of platelet-derived growth factor A-chain and c-myc gene expressions by angiotensin II in cultured rat vascular smooth muscle cells. Journal of Clinical Investigation. https://doi.org/10.1172/jci114032
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