The DOI matches its Crossref record; 73 of the 73 references it cites were found in a registry or an index.
pp60c-src activation in human colon carcinoma.
Open accessAbstract
We measured the in vitro protein-tyrosine kinase activity of pp60c-src from human colon carcinoma cell lines and tumors. The activity of pp60c-src from six of nine carcinoma cell lines was higher (on average, fivefold as measured by enolase phosphorylation, or eightfold as measured by autophosphorylation) than that of pp60c-src from normal colonic mucosal cells, or human or rodent fibroblasts. Similarly, the activity of pp60c-src from 13 of 21 primary colon carcinomas was five- or sevenfold higher than that of pp60c-src from normal colonic mucosa adjacent to the tumor. The increased pp60c-src activity did not result solely from an increase in the level of pp60c-src protein, suggesting the specific activity of the pp60c-src kinase is elevated in the tumor cells. pp60c-src from colon carcinoma cells and normal colonic mucosal cells was phosphorylated at similar sites. We used immunoblotting with antibodies to phosphotyrosine to identify substrates of protein-tyrosine kinases in colonic cells. Three phosphotyrosine-containing proteins were detected at significantly higher levels in most colon carcinoma cell lines than in normal colonic mucosal cells or human or rat fibroblasts. All colon carcinoma cell lines with elevated pp60c-src in vitro kinase activity, showed increased phosphorylation of proteins on tyrosine in vivo, suggesting the presence of an activated protein-tyrosine kinase(s).
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“Activation of SFKs occurs with high frequency during the development of CRC.”
From Dasatinib sensitizes KRAS mutant colorectal tumors to cetuximab (2011) · cites this paper as Cartwright et al., 1989
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“An increase in SFK activity in CRC tumors as compared to normal adjacent mucosa has been reported (Bolen et al., 1987; Cartwright et al., 1989).”
From Dasatinib sensitizes KRAS mutant colorectal tumors to cetuximab (2011) · cites this paper as Cartwright et al., 1989
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Expression of Cellular Oncogenes in Human Malignancies
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Role of Src expression and activation in human cancer
Irby, R., & Yeatman, T. J. (2000) · Oncogene · doi:10.1038/sj.onc.1203912 · Cited by 878
Src family kinases in tumor progression and metastasis
Summy, J. M., & Gallick, G. E. (2003) · Cancer and Metastasis Reviews · doi:10.1023/a:1023772912750 · Cited by 827
Kim, H., Jen, J., Vogelstein, B., & Hamilton, S. R. (1994) · PubMed Central · Cited by 809
Src in cancer: deregulation and consequences for cell behaviour
Frame, M. C. (2002) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/s0304-419x(02)00040-9 · Cited by 696
Activating SRC mutation in a subset of advanced human colon cancers
Irby, R. B., Mao, W., Coppola, D., Kang, J., Loubeau, J. M., Trudeau, W., Karl, R., Fujita, D. J., Jove, R., & Yeatman, T. J. (1999) · Nature Genetics · doi:10.1038/5971 · Cited by 489
Metadata
- Authors
- Cartwright, C. A., Kamps, M. P., Meisler, A. I., Pipas, J. M., & Eckhart, W.
- Affiliations
- Salk Institute for Biological Studies (US)
- ORCID
- Pipas, J M
- Venue
- Journal of Clinical Investigation
- Published
- 1989-06-01
- Volume
- 83
- Issue
- 6
- Pages
- 2025-2033
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC303927
- OpenAlex
- W2121881330
- Cited by
- 265
- References
- 72
- Citations by year
- 2024: 2 · 2023: 5 · 2022: 2 · 2021: 3 · 2020: 2 · 2018: 2 · 2017: 5 · 2016: 5 · 2015: 4 · 2014: 8 · 2013: 5 · 2012: 8
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA 14195, CA-13884, CA-01040)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Mechanisms and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Keywords
- Src kinase, tyrosine kinase, colon cancer, kinase activation, autophosphorylation, colonic mucosa, cancer cell lines, oncogene expression
- MeSH terms
- Carcinoma, Cell Line, Colonic Neoplasms, Enzyme Activation, Humans, Intestinal Mucosa, Phosphoproteins, Phosphorylation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Retroviridae Proteins, Tumor Cells, Cultured, Tyrosine, Oncogene Protein pp60(v-src)
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