The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Increases of intracellular magnesium promote glycodeoxycholate-induced apoptosis in rat hepatocytes.
Open accessAbstract
Retention of bile salts by the hepatocyte contributes to liver injury during cholestasis. Although cell injury can occur by one of two mechanisms, necrosis versus apoptosis, information is lacking regarding apoptosis as a mechanism of cell death by bile salts. Our aim was to determine if the bile salt glycodeoxycholate (GDC) induces apoptosis in rat hepatocytes. Morphologic assessment included electron microscopy and quantitation of nuclear fragmentation by fluorescent microscopy. Biochemical studies included measurements of DNA fragmentation, in vitro endonuclease activity, cytosolic free Ca2+ (Cai2+), and cytosolic free Mg2+ (Mgi2+). Morphologic studies demonstrated typical features of apoptosis in GDC (50 microM) treated cells. The "ladder pattern" of DNA fragmentation was also present in DNA obtained from GDC-treated cells. In vitro endonuclease activity was 2.5-fold greater with Mg2+ than Ca2+. Although basal Cai2+ values did not change after addition of GDC, Mgi2+ increased twofold. Incubation of cells in an Mg(2+)-free medium prevented the rise in Mgi2+ and reduced nuclear and DNA fragmentation. In conclusion, GDC induces apoptosis in hepatocytes by a mechanism promoted by increases of Mgi2+ with stimulation of Mg(2+)-dependent endonucleases. These data suggest for the first time that changes of Mgi2+ may participate in the program of cellular events culminating in apoptosis.
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Molecular aspects of hepatobiliary transport
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Metadata
- Authors
- Patel, T., Bronk, S. F., & Gores, G. J.
- Venue
- Journal of Clinical Investigation
- Published
- 1994-12-01
- Volume
- 94
- Issue
- 6
- Pages
- 2183-2192
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1976773018
- Cited by
- 270
- References
- 50
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Patel, T., Bronk, S. F., & Gores, G. J. (1994). Increases of intracellular magnesium promote glycodeoxycholate-induced apoptosis in rat hepatocytes. Journal of Clinical Investigation. https://doi.org/10.1172/jci117579
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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