Cited by 275Immune Response and InflammationEscherichia coli research studiesImmunotherapy and Immune Responses

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A novel Escherichia coli lipid A mutant that produces an antiinflammatory lipopolysaccharide.

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Somerville, J E, Cassiano, L, Bainbridge, B, Cunningham, M D, Darveau, R P·1996·Journal of Clinical Investigation

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Abstract

A unique screen was used to identify mutations in Escherichia coli lipid A biosynthesis that result in a decreased ability to stimulate E-selectin expression by human endothelial cells. A mutation was identified in the msbB gene of E. coli that resulted in lipopolysaccharide (LPS) that lacks the myristoyl fatty acid moiety of the lipid A. Unlike all previously reported lipid A mutants, the msbB mutant was not conditionally lethal for growth. Viable cells or purified LPS from an msbB mutant had a 1000-10,000-fold reduction in the ability to stimulate E-selectin production by human endothelial cells and TNF alpha production by adherent monocytes. The cloned msbB gene was able to functionally complement the msbB mutant, restoring both the LPS to its native composition and the ability of the strain to stimulate immune cells. Nonmyristoylated LPS acted as an antagonist for E-selectin expression when mixed with LPS obtained from the parental strain. These studies demonstrate a significant role for the myristate component of LPS in immune cell activation and antagonism. In addition, the msbB mutant allowed us to directly examine the crucial role that the lipid A structure plays when viable bacteria are presented to host defense cells.

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Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
Yanisch-Perron, C., Vieira, J., & Messing, J. W. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 14,909
CD14, a Receptor for Complexes of Lipopolysaccharide (LPS) and LPS Binding Protein
Wright, S. D., Ramos, R., Tobias, P. S., Ulevitch, R. J., & Mathison, J. C. (1990) · Science · doi:10.1126/science.1698311 · Cited by 3,996
[10] Assay of inorganic phosphate, total phosphate and phosphatases
Ames, B. N. (1966) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(66)08014-5 · Cited by 3,338
Bacterial lipopolysaccharides : extraction with phenol-water and further applications of the procedure
Westphal, O. (1965) · Medical Entomology and Zoology · Cited by 2,533
Identification of a protein required for disulfide bond formation in vivo.
Bardwell, J., McGovern, K., & Beckwith, J. (1991) · Cell · doi:10.1016/0092-8674(91)90532-4 · Cited by 961
Lipopolysaccharide activation of human endothelial and epithelial cells is mediated by lipopolysaccharide-binding protein and soluble CD14.
Pugin, J., Schürer-Maly, C., Leturcq, D., Moriarty, A., Ulevitch, R., & Tobias, P. (1993) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.90.7.2744 · Cited by 901
Soluble CD14 participates in the response of cells to lipopolysaccharide.
Frey, E. A., Miller, D. S., Jahr, T. G., Sundan, A., Bažil, V., Espevik, T., Finlay, B. B., & Wright, S. D. (1992) · The Journal of Experimental Medicine · doi:10.1084/jem.176.6.1665 · Cited by 682
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance
Jorgensen, R. A., Rothstein, S. J., & Reznikoff, W. S. (1979) · Molecular and General Genetics MGG · doi:10.1007/bf00267254 · Cited by 577

Show all 31 references

Research works printed but not linked: 1

Takada, H., and S. Kotani. 1992. Structure-function relationships of lipid A. In Bacterial endotoxic lipopolysaccharides. Volume I. Molecular Biochemis- try and Cellular Biology. D. C. Morrison and J. L. Ryan, editors. CRC Press, Boca Raton, FL. 107-130.

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Authors: Somerville, J. E., Cassiano, L., Bainbridge, B., Cunningham, M. D., & Darveau, R. P.
Affiliations: Bristol-Myers Squibb (United States) (US)
ORCID: Cassiano, L
Cunningham, M D
Darveau, R P
Venue: Journal of Clinical Investigation
Publisher: American Society for Clinical Investigation
Published: 1996-01-15
Volume: 97
Issue: 2
Pages: 359-365
ISSN: 0021-9738 (ISSN-L)
DOI: 10.1172/jci118423
PMC: PMC507025
OpenAlex: W2006697711
Cited by: 275
References: 31
Citations by year: 2026: 4 · 2025: 5 · 2024: 7 · 2023: 10 · 2022: 8 · 2021: 3 · 2020: 6 · 2019: 5 · 2018: 6 · 2017: 10 · 2016: 5 · 2015: 6 · 2014: 7 · 2013: 11 · 2012: 8
FWCI: 5.07
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Journal of Clinical Investigation (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Bristol-Myers Squibb
Topics: Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords: Escherichia coli, lipid A, lipopolysaccharide biosynthesis, E-selectin, endothelial cells, TNF-α, monocytes, immune cell activation, complementation
MeSH terms: Acyltransferases, Cells, Cultured, Endothelium, Vascular, Escherichia coli, Fatty Acids, Humans, Isomerases, Lipid A, Lipopolysaccharides, Myristic Acids, Structure-Activity Relationship, Tumor Necrosis Factor-alpha, Mutagenesis, E-Selectin, Protein Disulfide-Isomerases, Myristic Acid, Escherichia coli Proteins

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A novel Escherichia coli lipid A mutant that produces an antiinflammatory lipopolysaccharide.

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