The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
A novel Escherichia coli lipid A mutant that produces an antiinflammatory lipopolysaccharide.
Open accessAbstract
A unique screen was used to identify mutations in Escherichia coli lipid A biosynthesis that result in a decreased ability to stimulate E-selectin expression by human endothelial cells. A mutation was identified in the msbB gene of E. coli that resulted in lipopolysaccharide (LPS) that lacks the myristoyl fatty acid moiety of the lipid A. Unlike all previously reported lipid A mutants, the msbB mutant was not conditionally lethal for growth. Viable cells or purified LPS from an msbB mutant had a 1000-10,000-fold reduction in the ability to stimulate E-selectin production by human endothelial cells and TNF alpha production by adherent monocytes. The cloned msbB gene was able to functionally complement the msbB mutant, restoring both the LPS to its native composition and the ability of the strain to stimulate immune cells. Nonmyristoylated LPS acted as an antagonist for E-selectin expression when mixed with LPS obtained from the parental strain. These studies demonstrate a significant role for the myristate component of LPS in immune cell activation and antagonism. In addition, the msbB mutant allowed us to directly examine the crucial role that the lipid A structure plays when viable bacteria are presented to host defense cells.
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“In humans, hexa-acylated LPS potently activates TLR4 and host immunity, whereas hypo-acylated penta- and tetra-acylated LPS poorly activate TLR4 and can antagonize immune activation by hexa-acylated LPS6–9.”
From Gut microbiota-derived hexa-acylated lipopolysaccharides enhance cancer immunotherapy responses (2025) · cites this paper as [7]
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Yanisch-Perron, C., Vieira, J., & Messing, J. W. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 14,909
CD14, a Receptor for Complexes of Lipopolysaccharide (LPS) and LPS Binding Protein
Wright, S. D., Ramos, R., Tobias, P. S., Ulevitch, R. J., & Mathison, J. C. (1990) · Science · doi:10.1126/science.1698311 · Cited by 3,996
[10] Assay of inorganic phosphate, total phosphate and phosphatases
Ames, B. N. (1966) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(66)08014-5 · Cited by 3,338
Westphal, O. (1965) · Medical Entomology and Zoology · Cited by 2,533
Identification of a protein required for disulfide bond formation in vivo.
Bardwell, J., McGovern, K., & Beckwith, J. (1991) · Cell · doi:10.1016/0092-8674(91)90532-4 · Cited by 961
Pugin, J., Schürer-Maly, C., Leturcq, D., Moriarty, A., Ulevitch, R., & Tobias, P. (1993) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.90.7.2744 · Cited by 901
Soluble CD14 participates in the response of cells to lipopolysaccharide.
Frey, E. A., Miller, D. S., Jahr, T. G., Sundan, A., Bažil, V., Espevik, T., Finlay, B. B., & Wright, S. D. (1992) · The Journal of Experimental Medicine · doi:10.1084/jem.176.6.1665 · Cited by 682
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance
Jorgensen, R. A., Rothstein, S. J., & Reznikoff, W. S. (1979) · Molecular and General Genetics MGG · doi:10.1007/bf00267254 · Cited by 577
Research works printed but not linked: 1
- Takada, H., and S. Kotani. 1992. Structure-function relationships of lipid A. In Bacterial endotoxic lipopolysaccharides. Volume I. Molecular Biochemis- try and Cellular Biology. D. C. Morrison and J. L. Ryan, editors. CRC Press, Boca Raton, FL. 107-130.
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Raetz, C. R. H., & Whitfield, C. (2002) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.71.110601.135414 · Cited by 4,404
Lipid A Modification Systems in Gram-Negative Bacteria
Raetz, C. R. H., Reynolds, C. M., Trent, M. S., & Bishop, R. E. (2007) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.76.010307.145803 · Cited by 1,371
Gunn, J. S., Lim, K. B., Krueger, J., Kim, K., Guo, L., Hackett, M., & Miller, S. I. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.00757.x · Cited by 648
Lipid A Acylation and Bacterial Resistance against Vertebrate Antimicrobial Peptides
Guo, L., Lim, K. B., Poduje, C. M., Daniel, M., Gunn, J. S., Hackett, M., & Miller, S. I. (1998) · Cell · doi:10.1016/s0092-8674(00)81750-x · Cited by 645
The microbial challenge in periodontitis
Darveau, R. P., Tanner, A. C. R., & Page, R. C. (1997) · Periodontology 2000 · doi:10.1111/j.1600-0757.1997.tb00190.x · Cited by 633
Self-citation
Metadata
- Authors
- Somerville, J. E., Cassiano, L., Bainbridge, B., Cunningham, M. D., & Darveau, R. P.
- Affiliations
- Bristol-Myers Squibb (United States) (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1996-01-15
- Volume
- 97
- Issue
- 2
- Pages
- 359-365
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC507025
- OpenAlex
- W2006697711
- Cited by
- 275
- References
- 31
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 7 · 2023: 10 · 2022: 8 · 2021: 3 · 2020: 6 · 2019: 5 · 2018: 6 · 2017: 10 · 2016: 5 · 2015: 6 · 2014: 7 · 2013: 11 · 2012: 8
- FWCI
- 5.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Bristol-Myers Squibb
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- Escherichia coli, lipid A, lipopolysaccharide biosynthesis, E-selectin, endothelial cells, TNF-α, monocytes, immune cell activation, complementation
- MeSH terms
- Acyltransferases, Cells, Cultured, Endothelium, Vascular, Escherichia coli, Fatty Acids, Humans, Isomerases, Lipid A, Lipopolysaccharides, Myristic Acids, Structure-Activity Relationship, Tumor Necrosis Factor-alpha, Mutagenesis, E-Selectin, Protein Disulfide-Isomerases, Myristic Acid, Escherichia coli Proteins
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