The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Anaerobic killing of mucoid Pseudomonas aeruginosa by acidified nitrite derivatives under cystic fibrosis airway conditions
Open accessAbstract
Mucoid, mucA mutant Pseudomonas aeruginosa cause chronic lung infections in cystic fibrosis (CF) patients and are refractory to phagocytosis and antibiotics. Here we show that mucoid bacteria perish during anaerobic exposure to 15 mM nitrite (NO2) at pH 6.5, which mimics CF airway mucus. Killing required a pH lower than 7, implicating formation of nitrous acid (HNO2) and NO, that adds NO equivalents to cellular molecules. Eighty-seven percent of CF isolates possessed mucA mutations and were killed by HNO2 (3-log reduction in 4 days). Furthermore, antibiotic-resistant strains determined were also equally sensitive to HNO2. More importantly, HNO2 killed mucoid bacteria (a) in anaerobic biofilms; (b) in vitro in ultrasupernatants of airway secretions derived from explanted CF patient lungs; and (c) in mouse lungs in vivo in a pH-dependent fashion, with no organisms remaining after daily exposure to HNO2 for 16 days. HNO2 at these levels of acidity and NO2 also had no adverse effects on cultured human airway epithelia in vitro. In summary, selective killing by HNO2 may provide novel insights into the important clinical goal of eradicating mucoid P. aeruginosa from the CF airways.
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“Humans breathing nitric oxide gas exhibit increases in peripheral forearm blood flow, which is associated with increases in plasma nitrite.161”
From Nitrate-Nitrite-Nitric Oxide Pathway: Implications for Anesthesiology and Intensive Care (2010) · cites this paper as [161]
Experimental — accuracy not yet measuredIn the citing paper
“Work by Yoon et al demonstrated that acidified sodium nitrite mediated anaerobic killing of mucoid Pseudomonas aeruginosa in cultured biofilms and enhanced killing against this and antibiotic resistant strains in lungs in vivo [200].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [200]
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Endogenous Airway Acidification: Implications for Asthma Pathophysiology
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Pseudomonas aeruginosa Anaerobic Respiration in Biofilms
Yoon, S. S., Hennigan, R. F., Hilliard, G. M., Ochsner, U. A., Parvatiyar, K., Kamani, M. C., Allen, H. L., Dekievit, T., Gardner, P. R., Schwab, U. E., et al. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00295-2 · Cited by 587
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Chapter 4 Effects of Nitrogen Oxides on Chloride Channels
Gaston, B. M. (2008) · Current topics in membranes · doi:10.1016/s1063-5823(08)00204-4
a large asthma population Determinants of exhaled breath condensate pH in
Hunt, J. F., Gaston, B. M., Israel, E., Jarjour, W. N., Moore, W. C., Peters, S. P., Wenzel, S. E., Calhoun, W. J., Castro, M., Chung, K. F., et al. (2010)
Lewis, J. P., & Belvin, B. R. (2016) · Wiley eBooks · doi:10.1002/9781119004813.ch95
Short-chain Fatty Acids Modulate Bacterial Growth and Airway Epithelial Cell Inflammatory Responses
Ghorbani, P. (2012) · TSpace (University of Toronto)
Meyer, T. U. (2018) · Current Bioactive Compounds · doi:10.2174/1573407214666181119120027
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Role of Biofilm Formation in Mediation of Drug Resistance in E. Coli
Imtiaz, S., Saleem, S., Ahmad, I., Imran, M., Imtiaz, S., & Ch., S. (2022) · Pakistan Journal of Medical and Health Sciences · doi:10.53350/pjmhs22166155
3 Physiology of the Nose and Paranasal SinusesNose and paranasal sinuses
(2025) · Thieme Medical Publishers eBooks · doi:10.1055/b-0045-211958
Nitric Oxide Tunes Secreted Metabolite Bioactivity
Lonergan, Z. R., Weisflog, S. L., Scurria, M., Li, J., Thalhammer, K. O., Gutiérrez, O., Conway, S. J., & Newman, D. K. (2026) · Molecular Microbiology · doi:10.1111/mmi.70083
Park, H.-E., Kim, K.-M., Shin, J.-I., Choi, J.-G., An, W.-J., Trinh, M. P., Kang, K.-M., Yoo, J.-W., Byun, J.-H., Jung, M. H., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26708108
Metadata
- Authors
- Yoon, S. S.
- Affiliations
- University of Cincinnati (US)
- Corresponding author
- Yoon, S. S.
- ORCID
- Yoon, S. S.
- Venue
- Journal of Clinical Investigation
- Published
- 2006-01-19
- Volume
- 116
- Issue
- 2
- Pages
- 436-446
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC1350997
- OpenAlex
- W2134475541
- Cited by
- 212
- References
- 59
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 7 · 2021: 10 · 2020: 9 · 2019: 10 · 2018: 9 · 2017: 11 · 2016: 14 · 2015: 12 · 2014: 19 · 2013: 9 · 2012: 13
- FWCI
- 5.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
- YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
- UNC Libraries
- Funders
- U.S. Department of Energy (DEAC-02-98CH10886, -AC02-98 CH 10886, DE-AC02-)
- National Institutes of Health (5r01ai040541-05, DE-AC02-98CH10886)
- National Institute of General Medical Sciences (GM-69845, R01GM069845)
- National Institute of Allergy and Infectious Diseases (AI 40541, R01AI050812)
- Basic Energy Sciences (DE-AC02, DEAC0298CH10886, AC02-98CH10886)
- Brookhaven National Laboratory (AC-02-98CH10886, DE-AC02- 98CH10886)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Health Sciences › Medicine › Microbiology (medical) › Infections and bacterial resistance
- Keywords
- Pseudomonas aeruginosa, cystic fibrosis, nitrite, nitrous acid, nitric oxide, biofilm, airway mucus, pH dependence, antibiotic resistance, chronic lung infection, airway epithelium
- MeSH terms
- Anaerobiosis, Animals, Bacterial Proteins, Cells, Cultured, Child, Child, Preschool, Cystic Fibrosis, Epithelial Cells, Humans, Hydrogen-Ion Concentration, Infant, Microbial Sensitivity Tests, Mucus, Nitrites, Pseudomonas aeruginosa, Pseudomonas Infections, Sputum, Biofilms, Respiratory Mucosa, Drug Resistance, Bacterial, Mice
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