The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
PI3K signaling of autophagy is required for starvation tolerance and virulenceof Cryptococcus neoformans
Open accessAbstract
Autophagy is a process by which cells recycle cytoplasm and defective organelles during stress situations such as nutrient starvation. It can also be used by host cells as an immune defense mechanism to eliminate infectious pathogens. Here we describe the use of autophagy as a survival mechanism and virulence-associated trait by the human fungal pathogen Cryptococcus neoformans. We report that a mutant form of C. neoformans lacking the Vps34 PI3K (vps34Delta), which is known to be involved in autophagy in ascomycete yeast, was defective in the formation of autophagy-related 8-labeled (Atg8-labeled) vesicles and showed a dramatic attenuation in virulence in mouse models of infection. In addition, autophagic vesicles were observed in WT but not vps34Delta cells after phagocytosis by a murine macrophage cell line, and Atg8 expression was exhibited in WT C. neoformans during human infection of brain. To dissect the contribution of defective autophagy in vps34Delta C. neoformans during pathogenesis, a strain of C. neoformans in which Atg8 expression was knocked down by RNA interference was constructed and these fungi also demonstrated markedly attenuated virulence in a mouse model of infection. These results demonstrated PI3K signaling and autophagy as a virulence-associated trait and survival mechanism during infection with a fungal pathogen. Moreover, the data show that molecular dissection of such pathogen stress-response pathways may identify new approaches for chemotherapeutic interventions.
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Gutierrez, M. G., Master, S. S., Singh, S. B., Taylor, G. A., Colombo, M. I., & Deretic, V. (2004) · Cell · doi:10.1016/j.cell.2004.11.038 · Cited by 2,252
Phosphorylation and Functional Inactivation of TSC2 by Erk
Ma, L., Chen, Z., Erdjument-Bromage, H., Tempst, P., & Pandolfi, P. P. (2005) · Cell · doi:10.1016/j.cell.2005.02.031 · Cited by 1,268
Kihara, A., Noda, T., Ishihara, N., & Ohsumi, Y. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.152.3.519 · Cited by 1,004
Self-citationFormation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
Kirisako, T., Baba, M., Ishihara, N., Miyazawa, K., Ohsumi, M., Yoshimori, T., Noda, T., & Ohsumi, Y. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.2.435 · Cited by 881
Self-citationUrease as a Virulence Factor in Experimental Cryptococcosis
Cox, G. M., Mukherjee, J., Cole, G. T., Casadevall, A., & Perfect, J. R. (2000) · Infection and Immunity · doi:10.1128/iai.68.2.443-448.2000 · Cited by 522
Cryptococcus neoformans Is a Facultative Intracellular Pathogen in Murine Pulmonary Infection
Feldmesser, M., Kress, Y., Novikoff, P., & Casadevall, A. (2000) · Infection and Immunity · doi:10.1128/iai.68.7.4225-4237.2000 · Cited by 423
Tucker, S. C., & Casadevall, A. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.052702799 · Cited by 306
Autophagy is an immediate macrophage response to Legionella pneumophila
Amer, A. O., & Swanson, M. S. (2005) · Cellular Microbiology · doi:10.1111/j.1462-5822.2005.00509.x · Cited by 279
Laccase of Cryptococcus neoformans Is a Cell Wall-Associated Virulence Factor
Zhu, X., Gibbons, J., Garcia-Rivera, J., Casadevall, A., & Williamson, P. R. (2001) · Infection and Immunity · doi:10.1128/iai.69.9.5589-5596.2001 · Cited by 188
Self-citationChun, C. D., Liu, O. W., & Madhani, H. D. (2007) · PLoS Pathogens · doi:10.1371/journal.ppat.0030022 · Cited by 181
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Liu, X., Sempere, L. F., Ouyang, H., Memoli, V. A., Andrew, A. S., Luo, Y., Demidenko, E., Korc, M., Shi, W., Preis, M., et al. (2010) · Journal of Clinical Investigation · doi:10.1172/jci39566 · Cited by 373
Self-citationDNA methylation-associated silencing of tumor-suppressor microRNAs in cancer
Lopez-Serra, P., & Esteller, M. (2012) · Oncogene · doi:10.1038/onc.2011.354 · Cited by 360
Expanding fungal pathogenesis: Cryptococcus breaks out of the opportunistic box
Kronstad, J. W., Attarian, R., Cadieux, B., Choi, J., D'Souza, C. A., Griffiths, E. J., Geddes, J. M. H., Hu, G., Jung, W. H., Kretschmer, M., et al. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2522 · Cited by 340
Self-citationLin, C.-W., Chang, Y.-L., Chang, Y.-C., Lin, J.-C., Chen, C.-C., Pan, S.-H., Wu, C.-T., Chen, H.-Y., Yang, S.-C., Hong, T.-M., et al. (2013) · Nature Communications · doi:10.1038/ncomms2876 · Cited by 281
Metadata
- Authors
- Hu, G., Hacham, M., Waterman, S. R., Panepinto, J., Shin, S., Liu, X., Gibbons, J., Valyi-Nagy, T., Obara, K., Jaffe, H. A., Ohsumi, Y., & Williamson, P. R.
- Venue
- Journal of Clinical Investigation
- Published
- 2008-03-03
- Volume
- 118
- Issue
- 3
- Pages
- 1186-1197
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2111715441
- Cited by
- 220
- References
- 11
- FWCI
- 17.30
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Hu, G., Hacham, M., Waterman, S. R., Panepinto, J., Shin, S., Liu, X., Gibbons, J., Valyi-Nagy, T., Obara, K., Jaffe, H. A., Ohsumi, Y., & Williamson, P. R. (2008). PI3K signaling of autophagy is required for starvation tolerance and virulenceof Cryptococcus neoformans. Journal of Clinical Investigation. https://doi.org/10.1172/jci32053
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