The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Probing cell type–specific functions of Gi in vivo identifies GPCR regulators of insulin secretion
Open accessAbstract
The in vivo roles of the hundreds of mammalian G protein-coupled receptors (GPCRs) are incompletely understood. To explore these roles, we generated mice expressing the S1 subunit of pertussis toxin, a known inhibitor of G(i/o) signaling, under the control of the ROSA26 locus in a Cre recombinase-dependent manner (ROSA26(PTX)). Crossing ROSA26(PTX) mice to mice expressing Cre in pancreatic beta cells produced offspring with constitutive hyperinsulinemia, increased insulin secretion in response to glucose, and resistance to diet-induced hyperglycemia. This phenotype underscored the known importance of G(i/o) and hence of GPCRs for regulating insulin secretion. Accordingly, we quantified mRNA for each of the approximately 373 nonodorant GPCRs in mouse to identify receptors highly expressed in islets and examined the role of several. We report that 3-iodothyronamine, a thyroid hormone metabolite, could negatively and positively regulate insulin secretion via the G(i)-coupled alpha(2A)-adrenergic receptor and the G(s)-coupled receptor Taar1, respectively, and protease-activated receptor-2 could negatively regulate insulin secretion and may contribute to physiological regulation of glucose metabolism. The ROSA26(PTX) system used in this study represents a new genetic tool to achieve tissue-specific signaling pathway modulation in vivo that can be applied to investigate the role of G(i/o)-coupled GPCRs in multiple cell types and processes.
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Free fatty acids regulate insulin secretion from pancreatic β cells through GPR40
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Self-citationDysregulation of insulin receptor substrate 2 in β cells and brain causes obesity and diabetes
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Trace Amines and Their Receptors
Gainetdinov, R. R., Hoener, M. C., & Berry, M. D. (2018) · Pharmacological Reviews · doi:10.1124/pr.117.015305 · Cited by 384
Structure, function and pathophysiology of protease activated receptors
Adams, M. N., Ramachandran, R., Yau, M.-K., Suen, J. Y., Fairlie, D. P., Hollenberg, M. D., & Hooper, J. D. (2011) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2011.01.003 · Cited by 370
Short chain fatty acids and their receptors: new metabolic targets
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A chemical-genetic approach to study G protein regulation of β cell function in vivo
Guettier, J.-M., Gautam, D., Scarselli, M., de Azua, I. R., Li, J. H., Rosemond, E., Ma, X., Gonzalez, F. J., Armbruster, B. N., Lu, H., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906593106 · Cited by 344
Metadata
- Authors
- Regard, J. B., Kataoka, H., Cano, D. A., Camerer, E., Yin, L., Zheng, Y.-W., Scanlan, T. S., Hebrok, M., & Coughlin, S. R.
- Venue
- Journal of Clinical Investigation
- Published
- 2007-11-08
- Volume
- 117
- Issue
- 12
- Pages
- 4034-43
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2032216234
- Cited by
- 233
- References
- 6
- FWCI
- 14.07
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Regard, J. B., Kataoka, H., Cano, D. A., Camerer, E., Yin, L., Zheng, Y.-W., Scanlan, T. S., Hebrok, M., & Coughlin, S. R. (2007). Probing cell type–specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. Journal of Clinical Investigation. https://doi.org/10.1172/jci32994
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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