The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Polymorphisms of the 5' leader cistron of the human beta2-adrenergic receptor regulate receptor expression.
Open accessAbstract
Cellular expression of the beta2-adrenergic receptor (beta2AR) is controlled in part by a 19-amino acid peptide that regulates mRNA translation. This peptide is encoded by a short open reading frame, termed the 5' leader cistron (5'LC), which is 102 bp upstream of the beta2AR coding block. In 176 normal subjects we found a single nucleotide polymorphism resulting in either Arg (previously denoted wild-type) or Cys at position 19 of this peptide. Allele frequencies were 0.37 for Arg and 0.63 for Cys. To determine if these variants altered beta2AR expression, COS-7 cells were transfected with polymorphic constructs consisting of 1,989 bp encompassing the 5'LC and the beta2AR coding block exactly as found in the human gene. beta2AR density, as determined by [125I]CYP radioligand binding, was 72% higher in cells transfected with the 5'LC-Cys19 construct as compared with those transfected with the 5'LC-Arg19 construct and 110% higher when a cotransfection technique with a luciferase construct was used to control for transfection efficiency. Levels of the two mRNA transcripts were not different, confirming in vitro studies that the upstream peptide regulates receptor expression at the translational level. In human airway smooth muscle cells that natively express beta2AR, receptor expression was approximately twofold higher in those bearing the Cys versus the Arg polymorphism, confirming the phenotype in a relevant cell type. Linkage disequilibrium was observed between the 5'LC-Cys polymorphism and the beta2AR coding block polymorphisms Arg16 and Gln27 (P < 0.0001), although several different haplotypes were identified. Thus, beta2AR expression in the human population is controlled by a common polymorphism of this 5'LC, and may be responsible for interindividual variation in betaAR responsiveness.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
Green, S. A., Turki, J., Innis, M., & Liggett, S. B. (1994) · Biochemistry · doi:10.1021/bi00198a006 · Cited by 772
Self-citationArginine-Mediated RNA Recognition: the Arginine Fork
Calnan, B. J., Tidor, B., Biancalana, S., Hudson, D., & Frankel, A. D. (1991) · Science · doi:10.1126/science.252.5009.1167 · Cited by 744
Kobilka, B. K., Dixon, R. A., Frielle, T., Dohlman, H. G., Bolanowski, M. A., Sigal, I. S., Yang-Feng, T. L., Francke, U., Caron, M. G., & Lefkowitz, R. J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.1.46 · Cited by 679
Mutations in the Gene Encoding for the β 2 -adrenergic Receptor in Normal and Asthmatic Subjects
Reihsaus, E., Innis, M., MacIntyre, N., & Liggett, S. B. (1993) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb/8.3.334 · Cited by 617
Self-citationMartinez, F. D., Graves, P. E., Baldini, M., Solomon, S., & Erickson, R. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119874 · Cited by 605
Green, S. A., Cole, G., Jacinto, M., Innis, M., & Liggett, S. B. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49434-9 · Cited by 457
Self-citationGreen, S. A., Turki, J., Bejarano, P., Hall, I. P., & Liggett, S. B. (1995) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.13.1.7598936 · Cited by 439
Self-citationLarge, V, Hellström, L., Reynisdottir, S., Lönnqvist, F., Eriksson, P., Lannfelt, L., & Arner, P. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119854 · Cited by 420
Turki, J., Pak, J., Green, S. A., Martin, R. J., & Liggett, S. B. (1995) · Journal of Clinical Investigation · doi:10.1172/jci117838 · Cited by 397
Self-citation
Cited by
Drysdale, C. M., McGraw, D. W., Stack, C. B., Stephens, J. C., Judson, R. S., Nandabalan, K., Arnold, K., Ruano, G., & Liggett, S. B. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.19.10483 · Cited by 964
Self-citationISRAEL, E., DRAZEN, J. M., LIGGETT, S. B., BOUSHEY, H. A., CHERNIACK, R. M., CHINCHILLI, V. M., COOPER, D. M., FAHY, J. V., FISH, J. E., FORD, J. G., et al. (2000) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.162.1.9907092 · Cited by 549
Self-citationHigh-Throughput Genotyping with Single Nucleotide Polymorphisms
Ranade, K., Chang, M.-S., Ting, C.-T., Pei, D., Hsiao, C.-F., Olivier, M., Pesich, R., Hebert, J., Chen, Y.-D. I., Dzau, V. J., et al. (2001) · Genome Research · doi:10.1101/gr.157801 · Cited by 398
Peeters, R. P., van den Beld, A. W., Attalki, H., Toor, H. V., de Rijke, Y. B., Kuiper, G. G. J. M., Lamberts, S. W. J., Janssen, J. A. M. J. L., Uitterlinden, A. G., & Visser, T. J. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00571.2004 · Cited by 108
Metadata
- Authors
- McGraw, D. W., Forbes, S. L., Kramer, L. A., & Liggett, S. B.
- Venue
- Journal of Clinical Investigation
- Published
- 1998-12-01
- Volume
- 102
- Issue
- 11
- Pages
- 1927-1932
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1992530497
- Cited by
- 269
- References
- 23
- FWCI
- 5.85
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Polymorphisms of the 5' leader cistron of the human beta2-adrenergic receptor regulate receptor expression. https://app.cochat.ai/papers/10.1172/jci4862
- The paper
- McGraw, D. W., Forbes, S. L., Kramer, L. A., & Liggett, S. B. (1998). Polymorphisms of the 5' leader cistron of the human beta2-adrenergic receptor regulate receptor expression. Journal of Clinical Investigation. https://doi.org/10.1172/jci4862
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
