The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant
Open accessAbstract
Microparticles (MPs) resulting from vesiculation of platelets and other blood cells have been extensively documented in vitro and have been found in increased numbers in several vascular diseases, but little is known about MPs of endothelial origin. The aim of this study was to analyze morphological, immunological, and functional characteristics of MPs derived from human umbilical vein endothelial cells (HUVECs) stimulated by TNF, and to investigate whether these MPs are detectable in healthy individuals and in patients with a prothrombotic coagulation abnormality. Electron microscopy evidenced bleb formation on the membrane of TNF-stimulated HUVECs, leading to increased numbers of MPs released in the supernatant. These endothelial microparticles (EMPs) expressed the same antigenic determinants as the corresponding cell surface, both in resting and activated conditions. MPs derived from TNF-stimulated cells induced coagulation in vitro, via a tissue factor/factor VII-dependent pathway. The expression of E-selectin, ICAM-1, alphavbeta3, and PECAM-1 suggests that MPs have an adhesion potential in addition to their procoagulant activity. In patients, labeling with alphavbeta3 was selected to discriminate EMPs from those of other origins. We provide evidence that endothelial-derived MPs are detectable in normal human blood and are increased in patients with a coagulation abnormality characterized by the presence of lupus anticoagulant. Thus, MPs can be induced by TNF in vitro, and may participate in vivo in the dissemination of proadhesive and procoagulant activities in thrombotic disorders.
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Selectins: A family of adhesion receptors
Bevilacqua, M., Butcher, E., Furie, B., Furie, B., Gallatin, M., Gimbrone, M., Harlan, J., Kishimoto, K., Lasky, L., & McEver, R. (1991) · Cell · doi:10.1016/0092-8674(91)90174-w · Cited by 348
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“This coagulation abnormality is well recognized to be strongly associated with a thrombotic tendency (31, 32).”
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Effects of Severe Hypertension on Endothelial and Platelet Microparticles
Preston, R. A., Jy, W., Jimenez, J. J., Mauro, L. M., Horstman, L. L., Valle, M., Aime, G., & Ahn, Y. S. (2003) · Hypertension · doi:10.1161/01.hyp.0000049760.15764.2d · Cited by 499
Delivery of chemotherapeutic drugs in tumour cell-derived microparticles
Tang, K., Zhang, Y., Zhang, H., Xu, P., Liu, J., Ma, J., Lv, M., Li, D., Katirai, F., Shen, G.-X., et al. (2012) · Nature Communications · doi:10.1038/ncomms2282 · Cited by 453
Metadata
- Authors
- Combes, V., Simon, A.-C., Grau, G.-E., Arnoux, D., Camoin, L., Sabatier, F., Mutin, M., Sanmarco, M., Sampol, J., & Dignat-George, F.
- Venue
- Journal of Clinical Investigation
- Published
- 1999-07-01
- Volume
- 104
- Issue
- 1
- Pages
- 93-102
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2030383998
- Cited by
- 732
- References
- 3
- FWCI
- 8.15
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant. https://app.cochat.ai/papers/10.1172/jci4985
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- Combes, V., Simon, A.-C., Grau, G.-E., Arnoux, D., Camoin, L., Sabatier, F., Mutin, M., Sanmarco, M., Sampol, J., & Dignat-George, F. (1999). In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant. Journal of Clinical Investigation. https://doi.org/10.1172/jci4985
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
