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Cited by 108RNA Research and SplicingGlioma Diagnosis and TreatmentCancer-related molecular mechanisms research

RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3

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Han, Wei, Xin, Zhongshuai, Zhao, Zhiqiang, Bao, Wen, Lin, Xihua, Yin, Bin, Zhao, Jizong, Yuan, Jiangang +2 more·2013·Journal of Clinical Investigation
DOI 10.1172/jci61820PMC PMC3635714
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Abstract

PCBP2 is a member of the poly(C)-binding protein (PCBP) family, which plays an important role in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs in target mRNAs. Several PCBP family members have been reported to be involved in human malignancies. Here, we show that PCBP2 is upregulated in human glioma tissues and cell lines. Knockdown of PCBP2 inhibited glioma growth in vitro and in vivo through inhibition of cell-cycle progression and induction of caspase-3-mediated apoptosis. Thirty-five mRNAs were identified as putative PCBP2 targets/interactors using RIP-ChIP protein-RNA interaction arrays in a human glioma cell line, T98G. Four-and-a-half LIM domain 3 (FHL3) mRNA was downregulated in human gliomas and was identified as a PCBP2 target. Knockdown of PCBP2 enhanced the expression of FHL3 by stabilizing its mRNA. Overexpression of FHL3 attenuated cell growth and induced apoptosis. This study establishes a link between PCBP2 and FHL3 proteins and identifies a new pathway for regulating glioma progression.

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  • The direction of the influence of an RBP on the stability and translation efficiency of RNA can depend on the cell type and on the target RNA, as exemplified by PCBP2.

    “Nevertheless, it should be noted that the direction of the influence of an RBP on the stability and translation efficiency of RNA can depend on the cell type and on the target RNA, as exemplified by PCBP2: it can stabilize some mRNAs but promotes the degradation of other mRNAs [25,26,27].”

    From Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis (2024) · cites this paper as [26]

    The results (section labeled “Conflict of interest”; page unavailable) show that PCBP2 knockdown prolonged FHL3 mRNA half-life in T98G glioma cells, supporting the claim that PCBP2 can promote degradation of a target mRNA. The paper also cites work on stabilization of other mRNAs, but its own experiments do not establish that the direction of PCBP2’s effect depends on cell type. (LLM, conf=0.82)

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Cited by

Sorted by citations

Authors
Han, W., Xin, Z., Zhao, Z., Bao, W., Lin, X., Yin, B., Zhao, J., Yuan, J., Qiang, B., & Peng, X.
Affiliations
  • Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
  • Beijing Tian Tan Hospital (CN)
ORCID
Venue
Journal of Clinical Investigation
Publisher
American Society for Clinical Investigation
Published
2013-04-15
Volume
123
Issue
5
Pages
2103-2118
ISSN
0021-9738 (ISSN-L)
DOI
10.1172/jci61820
PMC
PMC3635714
OpenAlex
W2038520241
Cited by
108
References
0
Citations by year
2026: 4 · 2025: 5 · 2024: 6 · 2023: 9 · 2022: 7 · 2021: 11 · 2020: 8 · 2019: 7 · 2018: 9 · 2017: 6 · 2016: 9 · 2015: 16 · 2014: 11
FWCI
4.52
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Investigation (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Key Research and Development Program of China
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
Keywords
PCBP2, FHL3, glioma, RNA-binding proteins, mRNA stabilization, post-transcriptional regulation, cell cycle progression, caspase-3, apoptosis, T98G cells, RIP-Chip, RNA-protein interactions, glioma progression
MeSH terms
Animals, Brain Neoplasms, Cell Cycle, Flow Cytometry, Glioma, Humans, Mice, Nude, Neoplasm Transplantation, Gene Expression Regulation, Neoplastic, RNA-Binding Proteins, Apoptosis, Disease Progression, Cell Line, Tumor, Chromatin Immunoprecipitation, Intracellular Signaling Peptides and Proteins, Mice, Caspase 3, LIM Domain Proteins

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