The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Macrophages sustain HIV replication in vivo independently of T cells
Open accessAbstract
Macrophages have long been considered to contribute to HIV infection of the CNS; however, a recent study has contradicted this early work and suggests that myeloid cells are not an in vivo source of virus production. Here, we addressed the role of macrophages in HIV infection by first analyzing monocytes isolated from viremic patients and patients undergoing antiretroviral treatment. We were unable to find viral DNA or viral outgrowth in monocytes isolated from peripheral blood. To determine whether tissue macrophages are productively infected, we used 3 different but complementary humanized mouse models. Two of these models (bone marrow/liver/thymus [BLT] mice and T cell-only mice [ToM]) have been previously described, and the third model was generated by reconstituting immunodeficient mice with human CD34+ hematopoietic stem cells that were devoid of human T cells (myeloid-only mice [MoM]) to specifically evaluate HIV replication in this population. Using MoM, we demonstrated that macrophages can sustain HIV replication in the absence of T cells; HIV-infected macrophages are distributed in various tissues including the brain; replication-competent virus can be rescued ex vivo from infected macrophages; and infected macrophages can establish de novo infection. Together, these results demonstrate that macrophages represent a genuine target for HIV infection in vivo that can sustain and transmit infection.
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Cites
Exploring the full spectrum of macrophage activation
Mosser, D. M., & Edwards, J. P. (2008) · Nature Reviews Immunology · doi:10.1038/nri2448 · Cited by 9,144 · cited 1×
“While blood monocytes can be differentiated in vitro into macrophages, these cells lose much of their heterogeneity that results from anatomical location, which is critical in mimicking primary tissue macrophages (29, 30).”
BackgroundHeaton, R. K., Clifford, D. B., Franklin, D. R., Woods, S. P., Ake, C., Vaida, F., Ellis, R. J., Letendre, S. L., Marcotte, T. D., Atkinson, J. H., et al. (2010) · Neurology · doi:10.1212/wnl.0b013e318200d727 · Cited by 2,353
Origins of HIV and the AIDS Pandemic
Sharp, P. M., & Hahn, B. H. (2011) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a006841 · Cited by 1,496
Yang, J., Zhang, L., Yu, C., Yang, X.-F., & Wang, H. (2014) · Biomarker Research · doi:10.1186/2050-7771-2-1 · Cited by 1,099
Cited by
Pathogenesis of HIV-1 and Mycobacterium tuberculosis co-infection
Bell, L. C. K., & Noursadeghi, M. (2018) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2017.128 · Cited by 389
Wen, J., Wu, D., Qin, M., Liu, C., Wang, L., Xu, D., Vinters, H. V., Liu, Y., Kranz, E., Guan, X., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0434-z · Cited by 108
Deshiere, A., Joly-Beauparlant, C., Breton, Y., Ouellet, M., Raymond, F., Lodge, R., Barat, C., Roy, M.-A., Corbeil, J., & Tremblay, M. J. (2017) · Scientific Reports · doi:10.1038/s41598-017-05566-9 · Cited by 33
Metadata
- Authors
- Honeycutt, J. B., Wahl, A., Baker, C., Spagnuolo, R. A., Foster, J., Zakharova, O., Wietgrefe, S., Caro-Vegas, C., Madden, V., Sharpe, G., Haase, A. T., Eron, J. J., & Garcia, J. V.
- Venue
- Journal of Clinical Investigation
- Published
- 2016-03-07
- Volume
- 126
- Issue
- 4
- Pages
- 1353-1366
- ISSN
- 0021-9738 (ISSN-L)
- 1558-8238
- OpenAlex
- W2294972389
- Cited by
- 253
- References
- 4
- FWCI
- 26.30
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Honeycutt, J. B., Wahl, A., Baker, C., Spagnuolo, R. A., Foster, J., Zakharova, O., Wietgrefe, S., Caro-Vegas, C., Madden, V., Sharpe, G., Haase, A. T., Eron, J. J., & Garcia, J. V. (2016). Macrophages sustain HIV replication in vivo independently of T cells. Journal of Clinical Investigation. https://doi.org/10.1172/jci84456
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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