Comparative studies of steroidogenesis inhibitors (econazole, ketoconazole) on human breast cancer MCF-7 cell proliferation by growth experiments, thymidine incorporation and flow cytometric DNA analysis.
Abstract
The effects of aminoglutethimide, econazole and ketoconazole on human breast cancer cells in culture were compared with those of tamoxifen using four methods (growth experiments, thymidine incorporation, monoparameter and bivariate DNA content flow cytometry analysis). Aminoglutethimide (1 nM-10 microM) had no effect on cell proliferation after 8 days of treatment and did not decrease thymidine tritiated incorporation during logarithmic phase. Even at 20 microM, similar results were obtained with flow cytometry. Econazole and ketoconazole (1 nM-1 microM) decreased MCF-7 cell proliferation in a time- and dose-dependent fashion. They also decreased tritiated thymidine incorporation. By using flow cytometry and a monoclonal antibody against bromodeoxyuridine, we showed an accumulation of MCF-7 cells treated by imidazoles derivatives in G0-G1 phase of the cell cycle.
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The lack of inhibitory action of DHT in some MCF-7 cell lines is most likely due to a high level of 3α-HSD activity, preventing DHT from exerting its inhibitory effect before transformation into 3β-diol, a compound having intrinsic estrogenic activity.
“The lack of inhibitory action of DHT in some MCF-7 cell lines is most likely due to the presence of a high level of 3␣-HSD activity in these cells, thus preventing DHT from exerting its inhibitory effect before its transformation into 3-diol, a compound having intrinsic estrogenic activity (our unpublished data; and Ref. 273).”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [273]
Cites
Gratzner, H. G. (1982) · Science · doi:10.1126/science.7123245 · Cited by 2,653 · see citing works
A Human Cell Line From a Pleural Effusion Derived From a Breast Carcinoma 2
Soule, H. D., Vázquez, J., Long, A., Albert, S. M., & BRENNAN, M. D. (1973) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/51.5.1409 · Cited by 2,452 · see citing works
A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysis
Vindeløv, L. L., Christensen, I. J., & Nissen, N. I. (1983) · Cytometry · doi:10.1002/cyto.990030503 · Cited by 1,662 · see citing works
Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.
Dolbeare, F. A., Gratzner, H. G., Pallavicini, M. G., & Gray, J. W. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.18.5573 · Cited by 959 · see citing works
MATHEMATICAL ANALYSIS OF DNA DISTRIBUTIONS DERIVED FROM FLOW MICROFLUOROMETRY
Dean, P. N., & Jett, J. H. (1974) · The Journal of Cell Biology · doi:10.1083/jcb.60.2.523 · Cited by 799 · see citing works
Comparison of in vitro methods to determine drug-induced cell lethality.
Roper, P. R., & Drewinko, B. (1976) · PubMed · Cited by 273 · see citing works
Riccardi, A., Danova, M., Wilson, G. D., et al. (1988) · PubMed · Cited by 127 · see citing works
Cytotoxicity of ketoconazole in malignant cell lines
Rochlitz, C. F., Damon, L. E., Russi, M. B., et al. (1988) · Cancer Chemotherapy and Pharmacology · doi:10.1007/bf00264198 · Cited by 44 · see citing works
Jozan, S., Kreitmann, B., & Bayard, F. (1981) · European Journal of Endocrinology · doi:10.1530/acta.0.0980073 · Cited by 36 · see citing works
France, J. T., Mason, J. I., Magness, R. R., Murry, B. A., & Rosenfeld, C. R. (1987) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(87)90371-2 · Cited by 32 · see citing works
DiMattina, M., Maronian, N. C., Ashby, H., et al. (1988) · Fertility and Sterility · doi:10.1016/s0015-0282(16)59649-6 · Cited by 31 · see citing works
Endocrinology of breast cancer
Vorherr, H. (1987) · Maturitas · doi:10.1016/0378-5122(87)90061-2 · Cited by 29 · see citing works
Different therapeutic efficacy of ketoconazole in patients with Cushing's syndrome
Engelhardt, D., Jacob, K., & Doerr, H. G. (1989) · Journal of Molecular Medicine · doi:10.1007/bf01717326 · Cited by 28 · see citing works
Najid, A., Beneytout, J., & Tixier, M. (1989) · Cancer Letters · doi:10.1016/0304-3835(89)90021-9 · Cited by 24 · see citing works
Self-citationHigh dose ketoconazole: endocrine and therapeutic effects in postmenopausal breast cancer
Harris, A. L., Cantwell, B. M. J., & Dowsett, M. (1988) · British Journal of Cancer · doi:10.1038/bjc.1988.247 · Cited by 24 · see citing works
Inhibition of androgen aromatization in human breast cancer
Abul‐Hajj, Y. J. (1980) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(80)90049-7 · Cited by 19 · see citing works
Bruning, P. F., Bonfrer, J. M. G., Hart, A. A. M., et al. (1989) · European Journal of Cancer and Clinical Oncology · doi:10.1016/0277-5379(89)90032-1 · Cited by 17 · see citing works
Hormonal profiles in women with breast cancer (review).
Zumoff, B. (1988) · PubMed · Cited by 17 · see citing works
Restrepo, A., Stevens, D. A., & Utz, J. P. (1980) · Clinical Infectious Diseases · doi:10.1093/clinids/2.4.519 · Cited by 15 · see citing works
Lr, C., & Stocco, D. M. (1989) · PubMed · Cited by 10 · see citing works
Flow cytometric enumeration of drug-resistant tumor cells.
DeFazio, A., Musgrove, E. A., & Tattersall, M. H. N. (1988) · PubMed · Cited by 8 · see citing works
High-dose ketoconazole to untreated stage D prostate cancer
Aabo, K., Kjaer, M., & Hansen, H. H. (1988) · European Journal of Cancer and Clinical Oncology · doi:10.1016/s0277-5379(98)90013-x · Cited by 7 · see citing works
Najid, A. (1990) · European Journal of Cancer and Clinical Oncology · doi:10.1016/0277-5379(90)90636-8 · Cited by 5 · see citing works
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Labrie, F., Luu-The, V., Labrie, C., Bélanger, A., Simard, J., Lin, S.-X., & Pelletier, G. (2003) · Endocrine Reviews · doi:10.1210/er.2001-0031 · Cited by 544 · see citing works
- Authors
- Najid, A., & Ratinaud, M.-H.
- Affiliations
- Laboratoire de Biochimie (FR)
- Service Commuti de Cytofluorométrie, Université de Limoges, France
- Venue
- Tumori Journal
- Publisher
- SAGE Publications
- Published
- 1991-10
- Volume
- 77
- Issue
- 5
- Pages
- 385-390
- ISSN
- 0300-8916 (ISSN-L)
- 2038-2529
- OpenAlex
- W2409262814
- Cited by
- 12
- References
- 23
- Citations by year
- 2021: 1 · 2020: 2 · 2017: 1 · 2013: 1
- FWCI
- 0.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Tumori Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Breast Cancer Treatment Studies
- Keywords
- steroidogenesis inhibitors, econazole, ketoconazole, aminoglutethimide, tamoxifen, MCF-7 cells, breast cancer, cell proliferation, thymidine incorporation, DNA content, cell cycle arrest, G0/G1 phase, imidazole derivatives, anticancer agents
- MeSH terms
- Breast Neoplasms, Cell Division, DNA, Neoplasm, Drug Screening Assays, Antitumor, Econazole, Flow Cytometry, Humans, Ketoconazole, Thymidine, Tumor Cells, Cultured
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