Fabrication and characterization of collagen–hydroxyapatite-based composite scaffolds containing doxycycline via freeze-casting method for bone tissue engineering
Abstract
In this study, hydroxyapatite nanoparticles containing 10% doxycycline, a structural isomer of tetracycline, was prepared by the co-precipitation method. It was added to collagen solution for the preparation of the scaffold with freeze-casting method in order to develop a composite scaffold with both antibacterial and osteoinductive properties for repairing bone defects. The scaffolds were evaluated regarding their morphology, porosity, degradation and cellular response. The scaffolds for further investigation were added in a rat calvaria defect model. The study showed that after eight weeks, the bone formation was relatively higher in the collagen/nano-hydroxyapatite/doxycycline group with completely filled defect when compared with other groups. Histopathological evaluation showed that the defect in the collagen/nano-hydroxyapatite/doxycycline group was fully replaced by the new bone and connective tissue. Our results provide evidence supporting the possible applicability of doxycycline-containing scaffolds for successful bone regeneration.
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Pore size less than 100 μm hinders cell differentiation and causes cell aggregation.
“Pore size less than 100 µm hinders cell differentiation and also, causes cell aggregation [65].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [65]
Porosity was not hindered while incorporating nano-HA compared to collagen control (96.5%).
“The porosity was not hindered while incorporating nano-HA compared to collagen (control, 96.5%).”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [65]
Bone healing was enhanced in nano-HA/collagen compared to the collagen scaffold.
“Further, bone healing was enhanced in nano-HA/collagen than the collagen scaffold.[65]”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [65]
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References 50 · Cited by 45
- Authors
- Semyari, H., Salehi, M., Taleghani, F., Ehterami, A., Bastami, F., Jalayer, T., Semyari, H., Hamed Nabavi, M., & Semyari, H.
- Affiliations
- Shahed University (IR)
- Shahroud University of Medical Sciences (IR)
- Islamic Azad University, Tehran (IR)
- Shahid Beheshti University (IR)
- Oral and maxillofacial radiologist
- Faculty of dentistry, Shahed University, Tehran, Iran
- Corresponding author
- Semyari, Hassan
- ORCID
- Venue
- Journal of Biomaterials Applications
- Publisher
- SAGE Publications
- Published
- 2018-10
- Volume
- 33
- Issue
- 4
- Pages
- 501-513
- ISSN
- 0885-3282 (ISSN-L)
- 1530-8022
- OpenAlex
- W2896156618
- Cited by
- 45
- References
- 50
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 5 · 2021: 8 · 2020: 11 · 2019: 2
- FWCI
- 2.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomaterials Applications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Shahed University
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
- Keywords
- doxycycline, hydroxyapatite nanoparticles, freeze casting, bone tissue engineering, antibacterial properties, osteoinduction, bone regeneration, calvarial defect, scaffold degradation, porosity, co-precipitation
- MeSH terms
- Animals, Anti-Bacterial Agents, Bone Regeneration, Cells, Cultured, Collagen, Doxycycline, Male, Osteoblasts, Osteogenesis, Skull, Staphylococcus aureus, Porosity, Rats, Wistar, Durapatite, Tissue Engineering, Cell Proliferation, Nanoparticles, Tissue Scaffolds, Mesenchymal Stem Cells
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