High-Resolution Bioactivity Profiling of Mixtures toward the Acetylcholine Binding Protein Using a Nanofractionation Spotter Technology

Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors

Brejc, K., van Dijk, W. J., Klaassen, R. V., Schuurmans, M., John van der, O. O. S. T., Smit, A. B., & Sixma, T. K. (2001) · Nature · doi:10.1038/35077011 · Cited by 1,761 · see citing works

A glia-derived acetylcholine-binding protein that modulates synaptic transmission

Smit, A. B., Syed, N. I., Schaap, D., van Minnen, J., Klumperman, J., Kits, K. S., Lodder, H., van der Schors, R. C., van Elk, R., Sorgedrager, B., et al. (2001) · Nature · doi:10.1038/35077000 · Cited by 563 · see citing works

Nicotinic acetylcholine receptors: from structure to brain function

Hogg, R. C., Raggenbass, M., & Bertrand, D. (2003) · Springer eBooks · doi:10.1007/s10254-003-0005-1 · Cited by 529 · see citing works

Crystal structure of nicotinic acetylcholine receptor homolog AChBP in complex with an α-conotoxin PnIA variant

Celie, P. H. N., Kasheverov, I. E., Mordvintsev, D. Y., Hogg, R. C., van Nierop, P., van Elk, R., van Rossum-Fikkert, S. E., Zhmak, M. N., Bertrand, D., Tsetlin, V. I., et al. (2005) · Nature Structural & Molecular Biology · doi:10.1038/nsmb951 · Cited by 311 · see citing works

Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates

Ebada, S. S., Edrada‐Ebel, R., Lin, W., & Proksch, P. (2008) · Nature Protocols · doi:10.1038/nprot.2008.182 · Cited by 172 · see citing works

AChBP‐targeted α‐conotoxin correlates distinct binding orientations with nAChR subtype selectivity

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Nicotinic Acetylcholine Receptors as Drug Targets

Hogg, R. C., & Bertrand, D. (2004) · CNS & Neurological Disorders - Drug Targets · doi:10.2174/1568007043482507 · Cited by 113 · see citing works

Tryptophan Fluorescence Reveals Conformational Changes in the Acetylcholine Binding Protein

Hansen, S. B., Radić, Z., Talley, T. T., Molles, B. E., Deerinck, T. J., Tsigelny, I. F., & Taylor, P. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200462200 · Cited by 99 · see citing works

Advances in mass spectrometry-based post-column bioaffinity profiling of mixtures

Kool, J., Giera, M. A., Irth, H., & Niessen, W. M. A. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4406-0 · Cited by 70 · see citing works

Structure and Function of AChBP, Homologue of the Ligand‐Binding Domain of the Nicotinic Acetylcholine Receptor

Smit, A. B., Brejc, K., Syed, N. I., & Sixma, T. K. (2003) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1254.010 · Cited by 61 · see citing works

Microfractionation Revisited: A 1536 Well High Resolution Screening Assay

Giera, M. A., Heus, F., Janssen, L. E., Kool, J., Lingeman, H., & Irth, H. (2009) · Analytical Chemistry · doi:10.1021/ac900622b · Cited by 52 · see citing works

Determination and identification of estrogenic compounds generated with biosynthetic enzymes using hyphenated screening assays, high resolution mass spectrometry and off-line NMR

de Vlieger, J. S. B., Kolkman, A. J., Ampt, K. A. M., Commandeur, J. N. M., Vermeulen, N. P. E., Kool, J., Wijmenga, S. S., Niessen, W. M. A., Irth, H., & Honing, M. (2010) · Journal of Chromatography B · doi:10.1016/j.jchromb.2010.01.035 · Cited by 40 · see citing works

Online Fluorescence Enhancement Assay for the Acetylcholine Binding Protein with Parallel Mass Spectrometric Identification

Kool, J., de Kloe, G. E., Bruyneel, B., de Vlieger, J. S. B., Retra, K., Wijtmans, M., van Elk, R., Smit, A. B., Leurs, R., Lingeman, H., et al. (2010) · Journal of Medicinal Chemistry · doi:10.1021/jm100230k · Cited by 35 · see citing works

Development of an online p38α mitogen-activated protein kinase binding assay and integration of LC–HR-MS

Falck, D., de Vlieger, J. S. B., Niessen, W. M. A., Kool, J., Honing, M., Giera, M. A., & Irth, H. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4087-8 · Cited by 33 · see citing works

HPLC micro-fractionation coupled to a cell-based assay for automated on-line primary screening of calcium antagonistic components in plant extracts

Tammela, P., Wennberg, T., Vuorela, H. J., & Vuorela, P. M. (2004) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-004-2795-7 · Cited by 32 · see citing works

Development of a Novel Cytochrome P450 Bioaffinity Detection System Coupled Online to Gradient Reversed-Phase High-Performance Liquid Chromatography

Kool, J., Liempd, S. M. V., Ramautar, R., Schenk, T., Meerman, J. H. N., Irth, H., Commandeur, J. N. M., & Vermeulen, N. P. E. (2005) · SLAS DISCOVERY · doi:10.1177/1087057105274904 · Cited by 30 · see citing works

Nanofractionation Spotter Technology for Rapid Contactless and High-Resolution Deposition of LC Eluent for Further Off-Line Analysis

Kool, J., de Kloe, G. E., Denker, A. D., van Altena, K., Smoluch, M. T., van Iperen, D. J., Nahar, T. T., Limburg, R. J., Niessen, W. M. A., Lingeman, H., et al. (2010) · Analytical Chemistry · doi:10.1021/ac102001g · Cited by 25 · see citing works

Bioactivities of Plectranthus ecklonii Constituents

Nyila, M. A., Leonard, C. M., Hussein, A. A., & Lall, N. (2009) · Natural Product Communications · doi:10.1177/1934578x0900400903 · Cited by 19 · see citing works

Reversed-phase liquid chromatography coupled on-line to estrogen receptor bioaffinity detection based on fluorescence polarization

Reinen, J., Kool, J., & Vermeulen, N. (2008) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-008-1833-2 · Cited by 19 · see citing works

Cytochrome P450 bio-affinity detection coupled to gradient HPLC: On-line screening of affinities to cytochrome P4501A2 and 2D6

Kool, J., Liempd, S. M. V., Harmsen, S., Beckman, J., van Elswijk, D. A., Commandeur, J. N. M., Irth, H., & Vermeulen, N. P. E. (2007) · Journal of Chromatography B · doi:10.1016/j.jchromb.2007.08.005 · Cited by 16 · see citing works

Screening and isolation of a natural dopamine D1 receptor antagonist using cell-based assays

Yu, S., Park, J., Paredes, V., Song, M., Baek, N., Lee, S., Lim, J.-S., Cho, N.-Y., Yoon, J., & Baek, K. (2009) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2009.12.005 · Cited by 5 · see citing works

Effect-directed analysis supporting monitoring of aquatic environments — An in-depth overview

Brack, W., Aı̈t-Aı̈ssa, S., Burgess, R. M., Busch, W., Creusot, N., Paolo, C. D., Escher, B. I., Hewitt, L. M., Hilscherová, K., Hollender, J., et al. (2016) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2015.11.102 · Cited by 414 · see citing works

Towards the review of the European Union Water Framework Directive: Recommendations for more efficient assessment and management of chemical contamination in European surface water resources

Brack, W., Dulio, V., Ågerstrand, M., Allan, I. J., Altenburger, R., Brinkmann, M., Bunke, D., Burgess, R. M., Cousins, I. T., Escher, B. I., et al. (2016) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2016.10.104 · Cited by 374 · see citing works

A Unifying Review of Bioassay-Guided Fractionation, Effect-Directed Analysis and Related Techniques

Weller, M. G. (2012) · Sensors · doi:10.3390/s120709181 · Cited by 187 · see citing works

Concepts and technologies for tracking bioactive compounds in natural product extracts: generation of libraries, and hyphenation of analytical processes with bioassays

Potterat, O., & Hamburger, M. O. (2013) · Natural Product Reports · doi:10.1039/c3np20094a · Cited by 130 · see citing works

Assembly of a π–π stack of ligands in the binding site of an acetylcholine-binding protein

Stornaiuolo, M., de Kloe, G. E., Rucktooa, P., Fish, A., van Elk, R., Edink, E. S., Bertrand, D., Smit, A. B., de Esch, I. J. P., & Sixma, T. K. (2013) · Nature Communications · doi:10.1038/ncomms2900 · Cited by 68 · see citing works

Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products

Vitale, G. A., Geibel, C., Minda, V., Wang, M., Aron, A. T., & Petras, D. (2024) · Natural Product Reports · doi:10.1039/d3np00050h · Cited by 66 · see citing works

Combined Use of Extract Libraries and HPLC-Based Activity Profiling for Lead Discovery: Potential, Challenges, and Practical Considerations

Potterat, O., & Hamburger, M. O. (2014) · Planta Medica · doi:10.1055/s-0034-1382900 · Cited by 52 · see citing works

Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives

Malherbe, C. J., de Beer, D., & Joubert, E. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13033101 · Cited by 48 · see citing works

Drug Discovery on Natural Products: From Ion Channels to nAChRs, from Nature to Libraries, from Analytics to Assays

Otvos, R. A., Still, K. B. M., Somsen, G. W., Smit, A. B., & Kool, J. (2019) · SLAS DISCOVERY · doi:10.1177/2472555218822098 · Cited by 42 · see citing works

Rapid activity-directed screening of estrogens by parallel coupling of liquid chromatography with a functional gene reporter assay and mass spectrometry

Jonker, W., Lamoree, M. H., Houtman, C. J., Hamers, T., Somsen, G. W., & Kool, J. (2015) · Journal of Chromatography A · doi:10.1016/j.chroma.2015.06.012 · Cited by 36 · see citing works

Toward Streamlined Identification of Dioxin-like Compounds in Environmental Samples through Integration of Suspension Bioassay

Xiao, H., Brinkmann, M., Thalmann, B., Schiwy, A., Richter-Brockmann, S., Achten, C., Eichbaum, K., Gembé, C., Seiler, T., & Hollert, H. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.6b06003 · Cited by 22 · see citing works

EDA-EMERGE: an FP7 initial training network to equip the next generation of young scientists with the skills to address the complexity of environmental contamination with emerging pollutants

Brack, W., Govender, S., Schulze, T., Krauss, M. K. V., Hu, M., Muz, M., Hollender, J., Schirmer, K., Schollée, J. E., Hidasi, A. O., et al. (2013) · Environmental Sciences Europe · doi:10.1186/2190-4715-25-18 · Cited by 17 · see citing works

High-resolution metabolic profiling towards G protein-coupled receptors: Rapid and comprehensive screening of histamine H4 receptor ligands

Kool, J., Rudebeck, A. F., Fleurbaaij, F., Nijmeijer, S., Falck, D., Smits, R. A., Vischer, H. F., Leurs, R., & Niessen, W. M. A. (2012) · Journal of Chromatography A · doi:10.1016/j.chroma.2012.03.052 · Cited by 13 · see citing works

Development of a Profiling Strategy for Metabolic Mixtures by Combining Chromatography and Mass Spectrometry with Cell-Based GPCR Signaling

Nijmeijer, S., Vischer, H. F., Rudebeck, A. F., Fleurbaaij, F., Falck, D., Leurs, R., Niessen, W. M. A., & Kool, J. (2012) · SLAS DISCOVERY · doi:10.1177/1087057112451922 · Cited by 11 · see citing works

Virtual Screening against Acetylcholine Binding Protein

Utsintong, M., Rojsanga, P., Ho, K., Talley, T. T., Olson, A. J., Matsumoto, K., & Vajragupta, O. (2011) · SLAS DISCOVERY · doi:10.1177/1087057111421667 · Cited by 8 · see citing works

Methodologies for Effect‐Directed Analysis: Environmental Applications, Food Analysis, and Drug Discovery

Jonker, L. W., Lamoree, M. H., Houtman, C. J., & Kool, J. (2015) · Wiley eBooks · doi:10.1002/9783527673391.ch4 · Cited by 8 · see citing works

Development of an Online Cell-Based Bioactivity Screening Method by Coupling Liquid Chromatography to Flow Cytometry with Parallel Mass Spectrometry

Otvos, R. A., van Nierop, P., Niessen, W. M. A., Kini, R. M., Somsen, G. W., Smit, A. B., & Kool, J. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b00455 · Cited by 6 · see citing works

Acetylcholine-Binding Protein Affinity Profiling of Neurotoxins in Snake Venoms with Parallel Toxin Identification

Palermo, G., Schouten, W. M., Alonso, L. L., Ulens, C., Kool, J., & Slagboom, J. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242316769 · Cited by 4 · see citing works

High-Frequency Microfluidic Fractionation for Compound-Resolved Bioactivity-Based Metabolomics

Geibel, C., Schubert, J., Knoblauch, S. B., Hernández, A., Boldt, L., Schneider, D. C., Lambidis, S. P., Vitale, G. A., Fleischer, J., Haussmann, M., et al. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.5c04612 · Cited by 4 · see citing works

Pharmacological Characterization of Novel Histamine H4 Receptor Ligands

Nijmeijer, S. (2013) · Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam)

Novel transgenic medaka models to detect disruption of sex hormone signalling and gonadal development

Špirhanzlová, P. (2016) · doi:10.70675/cc692a7dzb965z4a0ezb234z60477cec9138

High-resolution bioactivity profiling of mixtures towards the acetylcholine binding protein aided by nanofractionation spotter technology.

Kool, J., Heus, F., de Kloe, G. E., Lingeman, H., Smit, A. B., Leurs, R., de Esch, I. J. P., Irth, H., & Niessen, W. M. A. (2011) · Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam)

Online Fluorescence Enhancement Assay for the Acetylcholine Binding Protein with Parallel Mass Spectrometric Identification

Kool, J., de Kloe, G. E., Bruyneel, B., de Vlieger, J. S. B., Retra, K., Wijtmans, M., van Elk, R., Smit, A. B., Leurs, R., Lingeman, H., et al. (2010) · Journal of Medicinal Chemistry · doi:10.1021/jm100230k · Cited by 35 · see citing works

Nanofractionation Spotter Technology for Rapid Contactless and High-Resolution Deposition of LC Eluent for Further Off-Line Analysis

Kool, J., de Kloe, G. E., Denker, A. D., van Altena, K., Smoluch, M. T., van Iperen, D. J., Nahar, T. T., Limburg, R. J., Niessen, W. M. A., Lingeman, H., et al. (2010) · Analytical Chemistry · doi:10.1021/ac102001g · Cited by 25 · see citing works

Online magnetic bead based dynamic protein affinity selection coupled to LC–MS for the screening of acetylcholine binding protein ligands

Pochet, L., Heus, F., Jonker, N., Lingeman, H., Smit, A. B., Niessen, W. M. A., & Kool, J. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.04.023 · Cited by 29 · see citing works

Bioanalysis of picomole amounts of acetylcholine by ion-pair partition chromatography applied to rat sciatic nerve

Ulin, B., Gustavii, K., & Persson, B. M. (1976) · Journal of Pharmacy and Pharmacology · doi:10.1111/j.2042-7158.1976.tb02834.x · Cited by 13 · see citing works