Use of Fructosyl Peptide Oxidase for HbA1c Assay
Open accessAbstract
ARKRAY, Inc developed the world's first automatic glycohemoglobin analyzer based on HPLC (1981). After that, ARKRAY developed enzymatic HbA1c assay "CinQ HbA1c" with the spread and diversification of HbA1c measurement (2007). CinQ HbA1c is the kit of Clinical Chemistry Analyzer, which uses fructosyl peptide oxidase (FPOX) for a measurement reaction. This report mainly indicates the developmental background, measurement principle, and future of the enzymatic method HbA1c reagent.
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Enzymatic methods are widely used in centralized laboratories and rely on endoglycosidase digestion followed by quantification of released glycated amino acids.
“In addition, although enzymatic methods are widely used in centralized laboratories and rely on endoglycosidase digestion followed by quantification of released glycated amino acids, no studies of enzymatic POC platforms met our inclusion criteria (42-44).”
From Comparison of point‑of‑care HbA1c devices with laboratory HPLC methods: A systematic review and meta‑analysis (2026) · cites this paper as [42]
The Abstract (page unavailable) describes an enzymatic HbA1c assay on an automatic analyzer: protease releases the glycated peptide fructosyl-Val-His, which is measured through an FPOX reaction. The Conclusion (page unavailable) says the method enables high-volume measurement on a clinical chemistry analyzer. These passages support an automated laboratory assay involving digestion and measurement of a glycated product, but they do not establish that enzymatic methods are widely used, and they specify protease and a glycated peptide—not endoglycosidase and released glycated amino acids. (LLM, conf=0.86)
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- Authors
- Yonehara, S., Inamura, N., Fukuda, M., & Sugiyama, K.
- Affiliations
- Arkray (Japan) (JP)
- Corresponding author
- Yonehara, Satoshi
- Venue
- Journal of Diabetes Science and Technology
- Publisher
- SAGE Publications
- Published
- 2015-01-28
- Volume
- 9
- Issue
- 2
- Pages
- 200-205
- ISSN
- 1932-2968 (ISSN-L)
- PMC
- PMC4604587
- OpenAlex
- W2135198587
- Cited by
- 16
- References
- 10
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2021: 1 · 2020: 1 · 2019: 4 · 2017: 2 · 2016: 2
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Diabetes Science and Technology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
- Keywords
- glycohemoglobin, clinical chemistry analyzers, diabetes monitoring
- MeSH terms
- Amino Acid Oxidoreductases, Blood Chemical Analysis, Diabetes Mellitus, Glycated Hemoglobin, Humans, Reagent Kits, Diagnostic, History, 20th Century, Enzyme Assays
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