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Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method.
Abstract
Acetone-fixed frozen sections of reactive lymph node, spleen, three B cell lymphomas, and three T cell lymphomas were studied for the presence of Ia-like antigens and two T cell antigens. Detection of the binding of the hybridoma monoclonal antibodies to these antigens took advantage of the biotin-avidin interaction. The detection method employed a biotin conjugate of goat anti-mouse antibody and an avidin conjugate of horseradish peroxidase. B cell lymphoma cells stained for Ia. The cell lymphomas were shown to be heterogeneous for the expression of the two T cell antigens. these three antigens were generally not detectable after 10% formalin fixation of B-5 fixation. Detection of these antigens and the method employed should prove useful in the immunologic categorization of human lymphomas.
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Cited by
The avidin-biotin complex in bioanalytical applications
Wilchek, M., & Bayer, E. A. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90120-0 · Cited by 894
Rogers, J., Luber-Narod, J., Styren, S. D., & Civin, W. H. (1988) · Neurobiology of Aging · doi:10.1016/s0197-4580(88)80079-4 · Cited by 743
Couch, Y., Alvarez-Erviti, L., Sibson, N. R., Wood, M. J., & Anthony, D. C. (2011) · Journal of Neuroinflammation · doi:10.1186/1742-2094-8-166 · Cited by 155
Metadata
- Authors
- Warnke, R., & Levy, R.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1980-08
- Volume
- 28
- Issue
- 8
- Pages
- 771-776
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2026351957
- Cited by
- 335
- References
- 0
- FWCI
- 22.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. https://app.cochat.ai/papers/10.1177/28.8.7003003
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- Warnke, R., & Levy, R. (1980). Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/28.8.7003003
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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