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Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.
Abstract
The recently developed low temperature embedding procedure with the resin Lowicryl K4M (Carlemalm E, Garavito M, Villiger W: Proc 7th Eur Cong Electron Microsc, 1980, p 656; Garavito M, Carlemalm E, Villiger W: Proc 7th Eur Cong Electron Microsc, 1980, p 658) was tested for its suitability for embedding of glutaraldehyde-fixed rat pancreatic tissue and for postembedding staining of thin sections with the protein A-gold (pAg) technique (Roth J, Bendayan M, Orci L: J Histochem Cytochem 26:1074, 1978) for amylase. Compared to conventional Epon embedding of glutaraldehyde fixed tissue, the low temperature embedding method with Lowicryl K4M resulted in a superior preservation of the general cellular fine structure, particularly in the Golgi apparatus. For low temperature embedded tissue, the quantitative evaluation of the immunocytochemical labeling for amylase showed a more specific staining of the rough endoplasmic reticulum, the Golgi apparatus, and the zymogen granules. This was due to a significant lowering of the background staining over all cellular organelles. The use of Lowicryl K4M at low temperature, due to the superior preservation, yields improved resolution and specificity in immunocytochemical postembedding staining.
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Citations
Cited by
Roth, J., & Berger, E. G. (1982) · The Journal of cell biology · doi:10.1083/jcb.93.1.223 · Cited by 619
Self-citationPlakoglobin: A protein common to different kinds of intercellular adhering junctions
Cowin, P., Kapprell, H.-P., Franke, W. W., Tamkun, J., & Hynes, R. O. (1986) · Cell · doi:10.1016/0092-8674(86)90706-3 · Cited by 503
Roth, J. (1985) · Cell · doi:10.1016/0092-8674(85)90034-0 · Cited by 453
Self-citationDomann, E., Wehland, J., Rohde, M., Pistor, S., Hartl, M., Goebel, W., Leimeister-Wächter, M., Wuenscher, M., & Chakraborty, T. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05252.x · Cited by 411
Lukinius, A., Wilander, E., Westermark, G. T., Engstr�m, U., & Westermark, P. (1989) · Diabetologia · doi:10.1007/bf00285291 · Cited by 371
Metadata
- Authors
- Roth, J., Bendayan, M., Carlemalm, E., Villiger, W., & Garavito, M.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1981-05
- Volume
- 29
- Issue
- 5
- Pages
- 663-671
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2157018895
- Cited by
- 705
- References
- 0
- FWCI
- 19.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- Roth, J., Bendayan, M., Carlemalm, E., Villiger, W., & Garavito, M. (1981). Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/29.5.6166664
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