Cited by 371Pancreatic function and diabetesNeuropeptides and Animal PhysiologyDiabetes and associated disorders

Co-localization of islet amyloid polypeptide and insulin in the B cell secretory granules of the human pancreatic islets

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Lukinius, A., Wilander, E., Westermark, G. T., Engstr�m, U., Westermark, P.·1989·Diabetologia

Abstract

Islet amyloid polypeptide is a novel 37 amino-acid-residues polypeptide which has been isolated from amyloid deposits in an insulinoma, and in human and cat islets of Langerhans. The molecule has 46% homology with the calcitonin gene-related peptide. Light microscopy examination of the pancreas shows that islet amyloid polypeptide immunoreactivity is restricted to the islet B cells. The present study utilized a rabbit antiserum against a synthetic peptide corresponding to positions 20-29 of islet amyloid polypeptide, a sequence without any amino-acid identity with calcitonin gene-related peptide. By applying the immunogold technique at the ultrastructural level, it was shown that both insulin and islet amyloid polypeptide immunoreactivity occurs in the central granular core of the human B cell secretory granules, while the A cells remain unlabelled. The demonstration that islet amyloid polypeptide is a granular protein of the B cells may indicate that it is released together with insulin. Further studies are necessary to evaluate the functional role of islet amyloid polypeptide.

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Research works printed but not linked: 2

  1. Sternberger LA (1979) Immunocytochemistry, second edn. John Wiley & Sons, New York Chichester Brisbane Toronto
  2. De Mey JR (1983) The preparation of immunoglobulin gold con- jugates (IGS reagents) and their use as markers for light and elec- tron microscopic immunocytochemistry. In: CuelloAC (ed) Immunohistochemistry, Vol 3. John Wiley & Sons, Chichester New York Brisbane Toronto Singapore, pp 347-372

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Authors
Lukinius, A., Wilander, E., Westermark, G. T., Engstr�m, U., & Westermark, P.
Affiliations
  • Uppsala University Hospital (SE)
  • Linköping University Hospital (SE)
  • Ludwig Cancer Research (SE)
Venue
Diabetologia
Published
1989-04
Volume
32
Issue
4
Pages
240-244
ISSN
  • 0012-186X (ISSN-L)
  • 1432-0428
OpenAlex
W2004210837
Cited by
371
References
28
Citations by year
2026: 5 · 2025: 3 · 2024: 8 · 2023: 1 · 2022: 5 · 2021: 7 · 2020: 4 · 2019: 9 · 2018: 9 · 2017: 5 · 2016: 6 · 2015: 11 · 2014: 7 · 2013: 9 · 2012: 8
FWCI
42.77
Type
journal article
Language
en
Open access
Locations
  • Diabetologia (publishedVersion)
  • PubMed (publishedVersion)
Funders
Medical Research Council
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
Keywords
amylin, insulin, B cells, secretory granules, colocalization, pancreatic islets, calcitonin gene-related peptide, insulinoma, amyloid deposits, A cells
MeSH terms
Aged, Amyloid, Cytoplasmic Granules, Female, Humans, Immunohistochemistry, Islets of Langerhans, Male, Microscopy, Electron, Middle Aged, Pancreatic Neoplasms

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