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Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry.
Abstract
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the CoCl2 + DAB is the ion of choice because: 1) it yields a distinct dark blue color that is easily distinguishable from brown DAB; 2) the blue reaction product is very stable throughout the entire staining procedure; and 3) background staining is minimal. These findings can be applied to the double staining technique of two different antigens in the same section. Among three staining procedures discussed, the avidin-biotin peroxidase complex (Co-DAB)-peroxidase-antiperoxidase (DAB) technique produced the best results because: 1) no antibody elution was needed following the avidin-biotin-peroxidase complex procedure when the CoCl2-DAB modification was used; and 2) no background staining occurred.
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Cited by
Fero, M. L., Rivkin, M., Tasch, M., Porter, P., Carow, C. E., Firpo, E., Polyak, K., Tsai, L.-H., Broudy, V., Perlmutter, R. M., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81239-8 · Cited by 1,457
Biotinylated dextran amine as an anterograde tracer for single- and double-labeling studies
Veenman, C. L., Reiner, A., & Honig, M. G. (1992) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(92)90089-v · Cited by 608
Steiner, J., Walter, M., Gos, T., Guillemin, G. J., Bernstein, H.-G., Sarnyai, Z., Mawrin, C., Brisch, R., Bielau, H., zu Schwabedissen, L., et al. (2011) · Journal of Neuroinflammation · doi:10.1186/1742-2094-8-94 · Cited by 568
Cullinan, W. E., Herman, J. P., & Watson, S. J. (1993) · Journal of Comparative Neurology · doi:10.1002/cne.903320102 · Cited by 541
Ben-Barak, Y., Russell, J., Whitnall, M., Ozato, K., & Gainer, H. (1985) · The Journal of Neuroscience · doi:10.1523/jneurosci.05-01-00081.1985 · Cited by 313
Metadata
- Authors
- Hsu, S. M., & Soban, E.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1982-10
- Volume
- 30
- Issue
- 10
- Pages
- 1079-1082
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2071230078
- Cited by
- 659
- References
- 0
- FWCI
- 14.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. https://app.cochat.ai/papers/10.1177/30.10.6182185
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- Hsu, S. M., & Soban, E. (1982). Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/30.10.6182185
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
