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Ventral subicular interaction with the hypothalamic paraventricular nucleus: Evidence for a relay in the bed nucleus of the stria terminalis

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Cullinan, William E., Herman, James P., Watson, Stanley J.·1993·Journal of Comparative Neurology
DOI 10.1002/cne.903320102
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Abstract

The axonal projections of the ventral subiculum to the bed nucleus of the stria terminalis (BST) were examined in the rat with the anterograde neuronal tracer Phaseolus vulgaris ‐ leucoagglutinin (PHA‐L). Axons originating in the ventral subiculum coursed to the BST through either the fimbria‐fornix, or a pathway involving the stria terminalis via the amygdala. Ventral subicular axons gave rise to dense terminal networks that were preferentially distributed in medial and ventral subregions of the BST. The distribution of subicular fibers and terminals was examined in relation to BST neurons that project to the hypothalamic paraventricular nucleus (PVN). In these cases, discrete iontophoretic injections of the retrograde tracer Fluoro‐gold were made in the PVN, with PHA‐L delivered to the ipsilateral ventral subiculum. An immunocytochemical double‐labeling protocol was then employed for the simultaneous detection of PHA‐L and Fluoro‐gold, and provided light microscopic evidence for subicular input to PVN‐projecting cells located within the BST. In a second series of experiments, the γ‐amino butyric acid (GABA)ergic nature of the BST was examined by in situ hybridization histochemistry for detection of transcripts encoding GAD 67 mRNA. The studies revealed that a high proportion of BST neurons express GAD 67 transcripts. Also, experiments combining Fluoro‐gold tracing with GAD 67 in situ hybridization suggested that a proportion of PVN‐projecting neurons in the BST are GABAergic. Taken together, the results of these sets of studies suggest that the inhibitory influences of the hippocampus on the PVN might be relayed through specific portions of the BST. These findings may have important implications for our understanding of the neural regulation of the hypothalamic‐pituitary‐adrenal axis. © 1993 Wiley‐Liss, Inc.

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  • Initiation of fear and sustained anxiety responses requires the recruitment of the BNST, which occurs partly as a result of direct innervation by BLA afferents, as well as through dense glutamatergic input from the hippocampus, mPFC, entorhinal cortex and insular cortex.

    “Initiation of fear and sustained anxiety responses requires the recruitment of the BNST7, which occurs partly as a result of direct innervation by BLA afferents, as well as through dense glutamatergic input from the hippocampus81,82, mPFC, entorhinal cortex and insular cortex55,83.”

    From Resolving the neural circuits of anxiety (2015) · cites this paper as [81]

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Research works printed but not linked: 6

  1. de Olmos, J.S. (1990) The amygdala. In G. Paxinos (ed): The Huinari Nervous System. New York: Academic Press, pp. 583-710.
  2. de Olmos, J.S., G.A. Alheid, and C.A. Beltramino (1985) Amygdala. In G. Paxinos (ed): The Rat Nervous System. New York: Academic Press, pp. 223-334.
  3. Gerfen, C., Sawchenko, P.E., and J . Carlsen (1989) Thcb PHA-L anterokmde axonal tracing method. In L. Heimer and L. Zaborszky (cds): Neuroana- tomical Tract-Tracing Methods. 2. Recent Progress. New York: Plenum Press, pp. 1 9 4
  4. Mugnani, E., and W.H. Oertel (1985) An atlas of the distribution of GABAergic neurons and terminals in the rat CNS as revealed by GAD immunohistochemistry. In A. Bjorklund and T. Hokfelt (eds): Handbook of Chemical Neuroanatomy, Vol. 4. GABA and Neuropeptides in the CNS, Part I. Amsterdam: Elsevier, pp. 436-622.
  5. Paxinos, G., and C. Watson (1986) The Rat Brain in Sterotaxic Coordinates, 2nd ed. Sydney: Academic Press.
  6. Swanson, L.W., C. Kahler, and A. Bjiirklund (1987) The limbic regon. I. Septohippocampal system. In A. Bjhklund, 'r. Hokfelt, and L.W. Swan- son (eds): Handbook of Chemical Neuroanatomy, Vol. 5: Integrated Systems ofthe CNS, Part I. Amsterdam: Elsevier, pp, 125-275.

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Authors
Cullinan, W. E., Herman, J. P., & Watson, S. J.
Affiliations
  • University of Michigan (US)
  • University of Kentucky (US)
ORCID
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
1993-06-08
Volume
332
Issue
1
Pages
1-20
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
DOI
10.1002/cne.903320102
OpenAlex
W2156147980
Cited by
541
References
103
Citations by year
2026: 8 · 2025: 5 · 2024: 29 · 2023: 12 · 2022: 16 · 2021: 13 · 2020: 18 · 2019: 15 · 2018: 13 · 2017: 15 · 2016: 19 · 2015: 21 · 2014: 18 · 2013: 25 · 2012: 20
FWCI
6.29
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 4141 d)
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • PubMed (publishedVersion)
  • Deep Blue (University of Michigan) (submittedVersion)
Funders
  • National Institutes of Health (5f32ns008267-02)
  • National Institute on Drug Abuse (DA00265-12)
  • National Institute of Diabetes and Digestive and Kidney Diseases (5T 32DK07245)
  • National Institute of Neurological Disorders and Stroke (NS08267)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Keywords
ventral subiculum, bed nucleus of the stria terminalis, hypothalamic paraventricular nucleus, hippocampal projections, GABAergic neurons, GAD67, hypothalamic-pituitary-adrenal axis, amygdala, axonal projections
MeSH terms
Amygdala, Animals, Axons, Axonal Transport, Brain Mapping, Fluorescent Dyes, gamma-Aminobutyric Acid, Glutamate Decarboxylase, Hippocampus, Immunoenzyme Techniques, Iontophoresis, Male, Nerve Tissue Proteins, Paraventricular Hypothalamic Nucleus, Phytohemagglutinins, Stilbamidines, Thalamus, Rats, Sprague-Dawley, In Situ Hybridization, Rats

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