A single-step, PCR-based method for the detection and differentiation of Plasmodium vivax and P. falciparum
Abstract
The ability to detect and differentiate between Plasmodium falciparum and P. vivax is of great importance for the routine laboratory diagnosis of malaria, donor-blood screening and epidemiological studies. Most PCR-based methods for the discrimination of these two species require nested protocols or an additional hybridization reaction, leading to high labour costs and long turn-around times. A simple, time-effective and yet sensitive and specific technique, based on a multiplex PCR, has now been developed for the simultaneous detection and differentiation of P. falciparum and P. vivax in blood samples. Compared with the 'gold standard' of microscopy, this method had a sensitivity and specificity of 100%, with a detection limit of just one P. falciparum or three P. vivax parasites/microl blood.
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Most published PCR assays are gel based with or without subsequent probe hybridizations.
“However, most published PCR assays are gel based with (4, 5, 15) or without (2, 3, 9, 23, 25-27, 29, 31, 35, 36) subsequent probe hybridizations”
From Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp (2005) · cites this paper as [27]
Cites
Barker, R. H., Banchongaksorn, T., Courval, J. M., Suwonkerd, W., Rimwungtragoon, K., & Wirth, D. F. (1992) · The American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1992.46.416 · Cited by 148 · cited 3× · see citing works
“The classic diag- Barker et al., 1992; Brown et al., 1992). nostic procedure remains the examination Initially, the target was the parasite’s repetitive of Giemsa-stained bloodsmears.”
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Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp
Mangold, K. A., Manson, R. U., Koay, E. S. C., Stephens, L., Regner, M., Thomson, R. B., Peterson, L. R., & Kaul, K. L. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.5.2435-2440.2005 · Cited by 278 · see citing works
- Authors
- Patsoula, E., Spanakos, G., Sofianatou, D., Parara, M., & Vakalis, N. C.
- Affiliations
- National School of Public Health (GR)
- NIMTS Hospital (GR)
- ORCID
- Patsoula, E.
- Venue
- Annals of Tropical Medicine & Parasitology
- Publisher
- Informa UK Limited
- Published
- 2003-01
- Volume
- 97
- Issue
- 1
- Pages
- 15-21
- ISSN
- 0003-4983 (ISSN-L)
- 1364-8594
- OpenAlex
- W2001978229
- Cited by
- 65
- References
- 1
- Citations by year
- 2026: 2 · 2024: 2 · 2023: 2 · 2022: 4 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 5
- FWCI
- 4.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annals of Tropical Medicine and Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- McMaster University
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- Plasmodium vivax, Plasmodium falciparum, multiplex PCR, malaria diagnosis, blood screening, sensitivity and specificity
- MeSH terms
- Animals, DNA Restriction Enzymes, Electrophoresis, Agar Gel, Humans, Malaria, Plasmodium falciparum, Plasmodium vivax, Sensitivity and Specificity, DNA, Protozoan, Polymerase Chain Reaction
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