CoChat
Sign Up
Cited by 202Malaria Research and ControlBacterial Identification and Susceptibility TestingBird parasitology and diseases

Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present

Padley, D., Moody, A.H., Chiodini, P.L., Saldanha, J.·2003·Annals of Tropical Medicine & Parasitology
DOI 10.1179/000349803125002977
Open this paper in CoChat — free account

Abstract

A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection.

Integrity signals

Registry

Crossref · 10.1179/000349803125002977

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Most published PCR assays are gel based with or without subsequent probe hybridizations.

    “However, most published PCR assays are gel based with (4, 5, 15) or without (2, 3, 9, 23, 25-27, 29, 31, 35, 36) subsequent probe hybridizations”

    From Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp (2005) · cites this paper as [26]

Cited by

Sorted by citations

Authors
Padley, D., Moody, A. H., Chiodini, P. L., & Saldanha, J.
Affiliations
  • National Institute for Biological Standards and Control (GB)
  • Hospital for Tropical Diseases (GB)
ORCID
Chiodini, P.L.
Venue
Annals of Tropical Medicine & Parasitology
Publisher
Informa UK Limited
Published
2003-03
Volume
97
Issue
2
Pages
131-137
ISSN
  • 0003-4983 (ISSN-L)
  • 1364-8594
DOI
10.1179/000349803125002977
OpenAlex
W2001645156
Cited by
202
References
0
Citations by year
2026: 7 · 2025: 6 · 2024: 4 · 2023: 7 · 2022: 16 · 2021: 11 · 2020: 8 · 2019: 10 · 2018: 12 · 2017: 14 · 2016: 16 · 2015: 9 · 2014: 12 · 2013: 18 · 2012: 12
FWCI
1.69
Type
journal article
Language
en
Open access
closed
Locations
  • Annals of Tropical Medicine and Parasitology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
Keywords
malaria diagnosis, Plasmodium species identification, multiplex PCR, Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale, mixed malaria infection, rapid molecular detection
MeSH terms
Animals, Humans, Malaria, Plasmodium, Plasmodium falciparum, Plasmodium malariae, Plasmodium vivax, Sensitivity and Specificity, DNA, Protozoan, Polymerase Chain Reaction, Parasitemia, Plasmodium ovale

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review