Mitochondrial DNA Targets Increase Sensitivity of Malaria Detection Using Loop-Mediated Isothermal Amplification
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Loop-mediated isothermal amplification (LAMP) of DNA offers the ability to detect very small quantities of pathogen DNA following minimal tissue sample processing and is thus an attractive methodology for point-of-care diagnostics. Previous attempts to diagnose malaria by the use of blood samples and LAMP have targeted the parasite small-subunit rRNA gene, with a resultant sensitivity for Plasmodium falciparum of around 100 parasites per μl. Here we describe the use of mitochondrial targets for LAMP-based detection of any Plasmodium genus parasite and of P. falciparum specifically. These new targets allow routine amplification from samples containing as few as five parasites per μl of blood. Amplification is complete within 30 to 40 min and is assessed by real-time turbidimetry, thereby offering rapid diagnosis with greater sensitivity than is achieved by the most skilled microscopist or antigen detection using lateral flow immunoassays.
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Several studies reported high sensitivity and specificity of LAMP compared with microscopy or PCR in nonpregnant populations.
“Several studies that compared the LAMP method to microscopy, PCR and RDT, reported high sensitivity and specificity compared with microscopy or PCR in nonpregnant populations [34–37].”
From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [34]
LAMP is a newer alternative to PCR and qPCR that does not require DNA purification, uses simple instrumentation, and can be completed in less than 1 hour.
“Loop-mediated isothermal amplification (LAMP) is a newer alternative to PCR and qPCR. This method does not require DNA purification, utilizes simple instrumentation (water bath or a heat block), and can be completed in less than 1 h [33,34].”
From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [34]
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- Authors
- Polley, S. D., Mori, Y., Watson, J., Perkins, M. D., González, I. J., Notomi, T., Chiodini, P. L., & Sutherland, C. J.
- Affiliations
- University College London Hospitals NHS Foundation Trust (GB)
- Eiken Chemical (Japan) (JP)
- FIND (CH)
- Foundation for Innovative New Diagnostics, Avenue de Budé 16, Geneva CH1202, Switzerland
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-08
- Volume
- 48
- Issue
- 8
- Pages
- 2866-2871
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC2916605
- OpenAlex
- W2144345536
- Cited by
- 167
- References
- 32
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 5 · 2023: 6 · 2022: 7 · 2021: 18 · 2020: 6 · 2019: 15 · 2018: 15 · 2017: 16 · 2016: 12 · 2015: 16 · 2014: 20 · 2013: 12 · 2012: 10
- FWCI
- 5.10
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University College London
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Keywords
- loop-mediated isothermal amplification, malaria diagnosis, Plasmodium falciparum, Plasmodium, point-of-care testing, small subunit rRNA gene, rapid molecular diagnostics
- MeSH terms
- DNA, Mitochondrial, Humans, Malaria, Plasmodium, Sensitivity and Specificity, Temperature, Time Factors, DNA, Protozoan, Nucleic Acid Amplification Techniques
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