Cited by 156Antioxidant Activity and Oxidative StressFree Radicals and AntioxidantsPhytochemicals and Antioxidant Activities

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

A method to measure antioxidant activity in organic media: application to lipophilic vitamins

Open access

Cano, A., Acosta, M., Arnao, M.B.·2000·Redox Report

DOI 10.1179/135100000101535933

Open this paper in CoChat — free account Open PDF

Abstract

The 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS(.+)) can be generated by the enzymatic system formed by hydrogen peroxide and horseradish peroxidase in an organic medium. The ABTS radical is easily generated in acidified ethanol medium in about 100 s with a stability of 1.7 x 10(-3) (-deltaabs/min) monitored at 730 nm. Other organic solvents, such as methanol or acetone, have lower radical generation times but the radical is less stable. The addition of Trolox or a lipophilic antioxidant such as alpha-tocopherol or beta-carotene produces a decrease in absorbance that can be used to estimate antioxidant capacity. Using a spectrophotometric end-point method and microplate-reader equipment, we have developed a method that estimates the antioxidant activity of different lipophilic vitamins. The use of Trolox as an antioxidant standard led to a limit of detection of 0.08 nmoles and limit of quantitation of 0.28 nmoles, while similar values were obtained for alpha-tocopherol and beta-carotene. The relative antioxidant activity values obtained by different antioxidants showed that alpha-tocopherol has a similar antioxidant potential to Trolox and that beta-carotene has 2.6 times the antioxidant potential of Trolox. In our opinion, this method can be useful for estimating the antioxidant activity in lipophilic samples and as a complement to other methods that measure antioxidant activity in aqueous media.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1179/135100000101535933

Cites

24 of 24 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Antioxidant activity applying an improved ABTS radical cation decolorization assay
Re, R., Pellegrini, N., Proteggente, A. R., Pannala, A. S., Yang, M. H., & Rice-Evans, C. A. (1999) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00315-3 · Cited by 26,986
A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates
Miller, N. J., Rice-Evans, C. A., Davies, M. J., Gopinathan, V., & Milner, A. D. (1993) · Clinical Science · doi:10.1042/cs0840407 · Cited by 3,108
Antioxidants in Human Health and Disease
Halliwell, B. B. (1996) · Annual Review of Nutrition · doi:10.1146/annurev.nu.16.070196.000341 · Cited by 1,852
Guidelines for data acquisition and data quality evaluation in environmental chemistry
MacDougall, D., Crummett, W. B., & al., E. (1980) · Analytical Chemistry · doi:10.1021/ac50064a004 · Cited by 1,634
Oxygen-radical absorbance capacity assay for antioxidants
Cao, G., Alessio, H. M., & Cutler, R. G. (1993) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(93)90027-r · Cited by 1,612
Antioxidant activities of carotenes and xanthophylls
Miller, N. J., Sampson, J., Candeias, L. P., Bramley, P. M., & Rice-Evans, C. A. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00323-7 · Cited by 1,030
[241 Total antioxidant status in plasma and body fluids
Rice-Evans, C. A., & Miller, N. J. (1994) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(94)34095-1 · Cited by 998
Antioxidant functions of carotenoids
Krinsky, N. I. (1989) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(89)90143-3 · Cited by 928
The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen
Childs, R. E., & Bardsley, W. G. (1975) · Biochemical Journal · doi:10.1042/bj1450093 · Cited by 895
Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures
van den Berg, R., Haenen, G. R. M. M., van den Berg, H., & Bast, A. (1999) · Food Chemistry · doi:10.1016/s0308-8146(99)00089-8 · Cited by 778

Show all 24 references

Research works printed but not linked: 4

Arnao MB, 1998, Rec Res Devel Agric Food Chem, 2, 893
Britton G, 1995, UVNis spectroscopy, 13
Campos AM, 1995, Bol Soc Chil Qufm, 40, 375
Romay C, 1996, Braz J Med Biol Res, 29, 175

Sorted by citations

Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements
Prior, R. L., Wu, X., & Schaich, K. M. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0502698 · Cited by 5,770
Analytical Methods Used in Determining Antioxidant Activity: A Review
Munteanu, I. G., & Apetrei, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073380 · Cited by 2,149
Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case
Arnao, M. B. (2000) · Trends in Food Science & Technology · doi:10.1016/s0924-2244(01)00027-9 · Cited by 616
Self-citation
ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways
Ilyasov, I. R., Белобородов, В. Л., Selivanova, I. A., & Terekhov, R. P. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031131 · Cited by 556
Understanding the chemistry behind the antioxidant activities of butylated hydroxytoluene (BHT): A review
Yehye, W. A., Rahman, N. A., Ariffin, A., Hamid, S. B. A., Alhadi, A. A., Kadir, F. A., & Yaeghoobi, M. (2015) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2015.06.026 · Cited by 509
A review on lignin antioxidants: Their sources, isolations, antioxidant activities and various applications
Lu, X., Gu, X., & Shi, Y. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.04.228 · Cited by 335
Development of a database for total antioxidant capacity in foods: a preliminary study
Wu, X., Gu, L., Holden, J. M., Haytowitz, D. B., Gebhardt, S. E., Beecher, G. R., & Prior, R. L. (2004) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2004.03.001 · Cited by 297
Total antioxidant capacity
Bartosz, G. (2003) · Advances in clinical chemistry · doi:10.1016/s0065-2423(03)37010-6 · Cited by 286
Correlation of total polyphenolic content with antioxidant and antibacterial activity of 24 extracts from Greek domestic Lamiaceae species
Stagos, D., Portesis, N., Spanou, C., Mossialos, D., Aligiannis, N., Chaita, E., Panagoulis, C., Reri, E., Skaltsounis, A., Tsatsakis, A. M., et al. (2012) · Food and Chemical Toxicology · doi:10.1016/j.fct.2012.08.033 · Cited by 203
In vitro antioxidant activity of tocopherols and tocotrienols and comparison of vitamin E concentration and lipophilic antioxidant capacity in human plasma
Müller, L., Theile, K., & Böhm, V. (2010) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200900399 · Cited by 191
Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea
Borges, A., José, H., Homem, V., & Simões, M. (2020) · Antibiotics · doi:10.3390/antibiotics9020048 · Cited by 184
“These observations were probably due to limitations of the DPPH method that include restricted reactivity towards antioxidants and solubility in aqueous and organic solvents [74].”
Method
The Influence of Different Electrical Conductivity Values in a Simplified Recirculating Soilless System on Inner and Outer Fruit Quality Characteristics of Tomato
Krauß, S., Schnitzler, W. H., Graßmann, J., & Woitke, M. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf051930a · Cited by 184
Assessment of polyphenolic content, antioxidant activity, protection against ROS-induced DNA damage and anticancer activity of Vitis vinifera stem extracts
Apostolou, A., Stagos, D., Galitsiou, E., Spyrou, A., Haroutounian, S., Portesis, N., Trizoglou, I., Hayes, A. W., Tsatsakis, A. M., & Kouretas, D. (2013) · Food and Chemical Toxicology · doi:10.1016/j.fct.2013.01.029 · Cited by 183
Hydrophilic and lipophilic antioxidant activity changes during on-vine ripening of tomatoes (Lycopersicon esculentum Mill.)
Cano, A., Acosta, M., & Arnao, M. B. (2003) · Postharvest Biology and Technology · doi:10.1016/s0925-5214(02)00141-2 · Cited by 170
Self-citation
Evaluation of the effect of high pressure on total phenolic content, antioxidant and antimicrobial activity of citrus peels
Casquete, R., Castro, S. M., Martı́n, A., Ruiz‐Moyano, S., Saraiva, J. A., Córdoba, M. D. G., & Teixeira, P. C. M. (2015) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2015.07.005 · Cited by 153
A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk.
Martysiak‐Żurowska, D., & Wenta, W. (2012) · PubMed · Cited by 149
Assessment of free radicals scavenging activity of seven natural pigments and protective effects in AAPH-challenged chicken erythrocytes
Zhang, J., Hou, X., Ahmad, H., Zhang, H., Zhang, L., & Wang, T. Z. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.08.025 · Cited by 139
Antibacterial and antioxidant activity of different types of honey derived from Mount Olympus in Greece
Stagos, D., Soulitsiotis, N., Tsadila, C., Papaeconomou, S., Arvanitis, C., Ntontos, A. C., Karkanta, F. K., Adamou‐Androulaki, S., Petrotos, K., Spandidos, D. A., et al. (2018) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2018.3656 · Cited by 138
Effects of Elevated CO2 on Grapevine (Vitis vinifera L.): Volatile Composition, Phenolic Content, and in Vitro Antioxidant Activity of Red Wine
Gonçalves, B. C., Falco, V., Moutinho‐Pereira, J., Bacelar, E. L. V. A., Peixoto, F., & Correia, C. M. (2008) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf8020199 · Cited by 137
Petals of Crocus sativus L. as a potential source of the antioxidants crocin and kaempferol
Zeka, K., Ruparelia, K. C., Continenza, M. A., Stagos, D., Vegliò, F., & Arroo, R. (2015) · Fitoterapia · doi:10.1016/j.fitote.2015.05.014 · Cited by 134
ABTS/TAC Methodology: Main Milestones and Recent Applications
Cano, A., Maestre-Hernández, A.-B., Hernández‐Ruíz, J., & Arnao, M. B. (2023) · Processes · doi:10.3390/pr11010185 · Cited by 134
Self-citation
Microencapsulation of extra‐virgin olive oil by spray‐drying: Influence of wall material and olive quality
Calvo, P., Hernández, T., Lozano, M., & González‐Gómez, D. (2010) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201000059 · Cited by 132
Influence of the microencapsulation on the quality parameters and shelf-life of extra-virgin olive oil encapsulated in the presence of BHT and different capsule wall components
Calvo, P., Núñez, Á. L. C., Lozano, M., & González‐Gómez, D. (2011) · Food Research International · doi:10.1016/j.foodres.2011.10.036 · Cited by 108
Opuntia ficus-indica Fruit: A Systematic Review of Its Phytochemicals and Pharmacological Activities
Giraldo-Silva, L., Ferreira, B., Rosa, E. A. S., & Dias, A. C. P. (2023) · Plants · doi:10.3390/plants12030543 · Cited by 98
A comparative study of the capsaicinoid and phenolic contents and in vitro antioxidant activities of the peppers of the genus Capsicum: an application of chemometrics
Sora, G. T. D. S., Haminiuk, C. W. I., da Silva, M. V., Zielinski, A. A. F., Gonçalves, G. A., Bracht, A., & Peralta, R. M. (2015) · Journal of Food Science and Technology · doi:10.1007/s13197-015-1935-8 · Cited by 98
Effects of microcapsule constitution on the quality of microencapsulated walnut oil
Calvo, P., Núñez, Á. L. C., Hernández, M. T., & González‐Gómez, D. (2011) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201100039 · Cited by 93
Evaluation of In Vitro Antioxidant and Antidiabetic Activities of Aristolochia longa Extracts
Omari, N. E., Sayah, K., Fettach, S., Blidi, O. E., Bouyahya, A., Faouzi, M. E. A., Kamal, R., & Barkiyou, M. (2019) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2019/7384735 · Cited by 89
Total and Sustainable Valorisation of Olive Pomace Using a Fractionation Approach
Ribeiro, T. B., Oliveira, A. L. S., Costa, C., Nunes, J., Vicente, A. A., & Pintado, M. (2020) · Applied Sciences · doi:10.3390/app10196785 · Cited by 84
Free radical-scavenging activity of indolic compounds in aqueous and ethanolic media
Cano, A., Alcaraz, O., & Arnao, M. B. (2003) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-003-1848-7 · Cited by 84
Self-citation
On-line antioxidant activity determination: comparison of hydrophilic and lipophilic antioxidant activity using the ABTS•+assay
Cano, A., Alcaraz, O., Acosta, M., & Arnao, M. B. (2002) · Redox Report · doi:10.1179/135100002125000334 · Cited by 82
Self-citation
Evaluation of different coloured carrot cultivars on antioxidative capacity based on their carotenoid and phenolic contents
Graßmann, J., Schnitzler, W. H., & Habegger, R. (2007) · International Journal of Food Sciences and Nutrition · doi:10.1080/09637480701359149 · Cited by 74
Determination of toxic perilla ketone, secondary plant metabolites and antioxidative capacity in five Perilla frutescens L. varieties
Müller-Waldeck, F., Sitzmann, J., Schnitzler, W. H., & Graßmann, J. (2009) · Food and Chemical Toxicology · doi:10.1016/j.fct.2009.10.009 · Cited by 69
Antioxidant activity of ginger extract and identification of its active components
Mošovská, S., Nováková, D., & Kaliňák, M. (2015) · Acta Chimica Slovaca · doi:10.1515/acs-2015-0020 · Cited by 69
Simulated digestion of an olive pomace water-soluble ingredient: relationship between the bioaccessibility of compounds and their potential health benefits
Ribeiro, T. B., Oliveira, A. L. S., Campos, D. A., Nunes, J., Vicente, A. A., & Pintado, M. (2020) · Food & Function · doi:10.1039/c9fo03000j · Cited by 69
Are olive pomace powders a safe source of bioactives and nutrients?
Ribeiro, T. B., Oliveira, A. L. S., Coelho, M. C., Veiga, M., Costa, E. M., Silva, S., Nunes, J., Vicente, A. A., & Pintado, M. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10812 · Cited by 65
The antiradical activity of some selected flavones and flavonols. Experimental and quantum mechanical study
Sroka, Z., Żbikowska, B., & Hładyszowski, J. (2015) · Journal of Molecular Modeling · doi:10.1007/s00894-015-2848-1 · Cited by 61
Antioxidant and antimicrobial activity of natural phenolic extract from defatted soybean flour by‐product for stone fruit postharvest application
Villalobos, M. C., Serradilla, M. J., Martı́n, A., Ordiales, E., Ruiz‐Moyano, S., & Córdoba, M. D. G. (2015) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.7327 · Cited by 61
Hydrophilic and Lipophilic Antioxidant Activity in Different Leaves of Three Lettuce Varieties
Cano, A., & Arnao, M. B. (2005) · International Journal of Food Properties · doi:10.1080/10942910500269584 · Cited by 58
Self-citation
Variability of chemical composition and antioxidant activity of essential oils between Myrtus communis var. Leucocarpa DC and var. Melanocarpa DC
Petretto, G. L., Maldini, M., Addis, R., Chessa, M., Foddai, M., Rourke, J. P., & Pintore, G. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.10.056 · Cited by 55
Bioavailability of Bioactive Molecules from Olive Leaf Extracts and its Functional Value
Martín‐Vertedor, D., Garrido, M., Pariente, J. A., Espino, J., & Delgado, J. (2016) · Phytotherapy Research · doi:10.1002/ptr.5625 · Cited by 53
The effects of lignin source and extraction on the composition and properties of biorefined depolymerization products
Obrzut, N., Hickmott, R., Shure, L., & Gray, K. A. (2023) · RSC Sustainability · doi:10.1039/d3su00262d · Cited by 50
Antioxidant capacity in fruit of Citrus cultivars with marked differences in pulp coloration: Contribution of carotenoids and vitamin C
Zacarías-García, J., Rey, F., Gil, J. V., Rodrigo, M. J., & Zacarı́as, L. (2020) · Food Science and Technology International · doi:10.1177/1082013220944018 · Cited by 50
Antioxidant and Skin Anti-Aging Effects of Marigold Methanol Extract
Kang, C. H., Rhie, S. J., & Kim, Y. C. (2018) · Toxicological Research · doi:10.5487/tr.2018.34.1.031 · Cited by 50
A Comparison of Carotenoid Content and Total Antioxidant Activity in Catsup from Several Commercial Sources in the United States
Ishida, B. K., & Chapman, M. H. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf040154o · Cited by 49
The effect of Luteolin on DNA damage mediated by a copper catalyzed Fenton reaction
Jomová, K., Hudecová, L., Lauro, P., Šimunková, M. M., Barbieriková, Z., Malček, M., Alwasel, S. H., Alhazza, I. M., Rhodes, C. J., & Valko, M. (2021) · Journal of Inorganic Biochemistry · doi:10.1016/j.jinorgbio.2021.111635 · Cited by 48
Determinação do conteúdo fenólico e avaliação da atividade antioxidante de Acacia podalyriifolia A. Cunn. ex G. Don, Leguminosae-mimosoideae
de Andrade, C. A., Costa, C. K., Bora, K., Miguel, M. D., Miguel, O. G., & Kerber, V. A. (2007) · Revista Brasileira de Farmacognosia · doi:10.1590/s0102-695x2007000200017 · Cited by 48
Wheat Germ Supplementation of a Low Vitamin E Diet in Rats Affords Effective Antioxidant Protection in Tissues
Leenhardt, F., Fardet, A., Lyan, B., Gueux, E., Rock, E., Mazur, A., Chanliaud, E., Demigné, C., & Rémésy, C. (2008) · Journal of the American College of Nutrition · doi:10.1080/07315724.2008.10719694 · Cited by 46
Immunostimulatory and antioxidant activities of a lignin isolated from Conocarpus erectus leaves
Santos, D. K. D. D. N., Barros, B. R. D. S., Aguiar, L. M. D. S., Filho, I. J. D. C., de Lorena, V. M. B., de Melo, C. M. L., & Napoleão, T. H. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.02.052 · Cited by 46
HPLC Analysis of Phenolics Compounds and Antioxidant Capacity of Leaves of Vitex megapotamica (Sprengel) Moldenke
de Brum, T. F., Zadra, M., Piana, M., Boligon, A. A., Frohlich, J. K., Freitas, R. D., Stefanello, S. T., Froeder, A. L. F., Belke, B. V., Nunes, L., et al. (2013) · Molecules · doi:10.3390/molecules18078342 · Cited by 44
Rational Design and Synthesis of New, High Efficiency, Multipotent Schiff Base-1,2,4-triazole Antioxidants Bearing Butylated Hydroxytoluene Moieties
Yehye, W. A., Rahman, N. A., Saad, O., Ariffin, A., Hamid, S. B. A., Alhadi, A. A., Kadir, F. A., Yaeghoobi, M., & Matlob, A. A. (2016) · Molecules · doi:10.3390/molecules21070847 · Cited by 43
Chemical composition and bioactivity of essential oils from flower and fruit of Thymbra capitata and Thymus species
Delgado, J., Garrido, M., Bote, E., Fuentes-Pérez, M. C., Espino, J., & Martín‐Vertedor, D. (2017) · Journal of Food Science and Technology · doi:10.1007/s13197-017-2617-5 · Cited by 44
Effects of a 12-Month Treatment with Glucagon-like Peptide-1 Receptor Agonists, Sodium-Glucose Cotransporter-2 Inhibitors, and Their Combination on Oxidant and Antioxidant Biomarkers in Patients with Type 2 Diabetes
Lambadiari, V., Thymis, J., Kouretas, D., Skaperda, Z., Tekos, F., Kousathana, F., Kountouri, A., Balampanis, K., Parissis, J., Andreadou, I., et al. (2021) · Antioxidants · doi:10.3390/antiox10091379 · Cited by 42
“When adding to the solution a substance with antioxidant activity, then the radical of ABTS • + is reduced with the addition of a hydrogen atom, resulting in decrease of the optical absorbance at 730 nm [38].”
Background
Effects of storage time and temperature on lipid oxidation of egg powders enriched with natural antioxidants
Matumoto‐Pintro, P. T., Murakami, A. E., Vital, A. C. P., Croge, C. P., da Silva, D. F., Ospina-Rojas, I. C., & Guerra, A. F. Q. G. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.02.044 · Cited by 42
Paradigm Shift in Phytochemicals Research: Evolution from Antioxidant Capacity to Anti-Inflammatory Effect and to Roles in Gut Health and Metabolic Syndrome
Xiao, L., Sun, Y., & Tsao, R. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c02326 · Cited by 50
Over 3 decades of research on dietary flavonoid antioxidants and cancer prevention: What have we achieved?
Alfa, H. H., & Arroo, R. (2019) · Phytochemistry Reviews · doi:10.1007/s11101-019-09632-0 · Cited by 40
Interplay between oxidative damage, the redox status, and metabolic biomarkers during long-term fasting
Grundler, F., Mesnage, R., Goutzourelas, N., Tekos, F., Makri, S., Brack, M., Kouretas, D., & de Toledo, F. W. (2020) · Food and Chemical Toxicology · doi:10.1016/j.fct.2020.111701 · Cited by 40
Antioxidant, Antihypertensive and Antimicrobial Properties of Phenolic Compounds Obtained from Native Plants by Different Extraction Methods
Boy, F. R., Casquete, R., Martínez, A. T., Córdoba, M. D. G., Ruiz‐Moyano, S., & Benito, M. J. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18052475 · Cited by 37
Oxidative Stability, Phenolic Compounds and Antioxidant Potential of a Virgin Olive Oil Enriched with Natural Bioactive Compounds
Delgado, J., Franco, M. N., Yuste, M. C. A., & Martín‐Vertedor, D. (2013) · Journal of Oleo Science · doi:10.5650/jos.ess13114 · Cited by 37
Mechanisms related to the anti-diabetic properties of mango (Mangifera indica L.) juice by-product
Rodríguez‐González, S., Gutiérrez-Ruíz, I. M., Pérez‐Ramírez, I. F., Mora, O., Ramos‐Gómez, M., & Reynoso‐Camacho, R. (2017) · Journal of Functional Foods · doi:10.1016/j.jff.2017.07.058 · Cited by 35
Biological Properties of Bee Bread Collected from Apiaries Located across Greece
Didaras, N. A., Kafantaris, I., Dimitriou, T. G., Mitsagga, C., Karatasou, K., Giavasis, I., Stagos, D., Amoutzias, G. D., Hatjina, F., & Mossialos, D. (2021) · Antibiotics · doi:10.3390/antibiotics10050555 · Cited by 35
TOMATO FRUIT YIELD AND QUALITY AS AFFECTED BY GRAFTING AND GROWING SYSTEM
Qaryouti, M. M., Qawasmi, W., Hamdan, H., & Edwan, M. (2007) · Acta Horticulturae · doi:10.17660/actahortic.2007.741.22 · Cited by 35
Effect of thermosonication on microorganisms, the antioxidant activity and the retinol level of human milk
Parreiras, P. M., Nogueira, J. A. V., da Cunha, L. R., Passos, M. C., Gomes, N. R., Breguez, G. S., Falco, T. S., Bearzoti, E., & Menezes, C. C. (2020) · Food Control · doi:10.1016/j.foodcont.2020.107172 · Cited by 33
Comparative Analysis between Synthetic Vitamin E and Natural Antioxidant Sources from Tomato, Carrot and Coriander in Diets for Market-Sized Dicentrarchus labrax
Pereira, R., Costa, M. M., Velasco, C., Cunha, L. M., Lima, R. C., Baião, L. F., Batista, S., Marques, A., Sá, T., Campos, D. A., et al. (2022) · Antioxidants · doi:10.3390/antiox11040636 · Cited by 33
Nutrient plasma levels achieved during treatment that reduces noise-induced hearing loss
Prell, C. G. L., Dolan, D. F., Bennett, D. C., & Boxer, P. A. (2011) · Translational research · doi:10.1016/j.trsl.2011.02.003 · Cited by 33
Toplam fenolik içerik ve antioksidan kapasite tayininde kullanilan baslica spektrofotometrik yöntemler
Büyüktuncel, E. (2013) · MARMARA PHARMACEUTCAL JOURNAL · doi:10.12991/201317377 · Cited by 33
Effect of Spinacia oleraceae L. and Perilla frutescens L. on Antioxidants and Lipid Peroxidation in an Intervention Study in Healthy Individuals
Schirrmacher, G., Skurk, T., Hauner, H., & Graßmann, J. (2010) · Plant Foods for Human Nutrition · doi:10.1007/s11130-009-0152-x · Cited by 32
Chemical structure elucidation and functional activities comparison of two polysaccharides purified from Citrus reticulata Blanco peels
Li, X., Chen, J., Yin, Y., Xiao, S., Zhang, R., Yang, Y., Li, L., Xu, H., Zhang, X., & Hu, P. (2024) · Chemical and Biological Technologies in Agriculture · doi:10.1186/s40538-024-00556-2 · Cited by 29
Evaluation of Antioxidant Activity in Foods with Special Reference to TEAC Method
Sharma, P. K., & Singh, R. (2013) · American Journal of Food Technology · doi:10.3923/ajft.2013.83.101 · Cited by 29
Tetracycline hydrochloride: A potential clinical drug for radioprotection
Alok, A., & Chaudhury, N. K. (2016) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2016.01.001 · Cited by 29
Antioxidant Activities and Phenolic Compounds of Several Tissues of Pawpaw ( Asimina triloba [L.] Dunal) Grown in Korea
Nam, J., Jang, H., & Rhee, Y. H. (2017) · Journal of Food Science · doi:10.1111/1750-3841.13806 · Cited by 25
Antioxidant capacity of the phenolic fraction and its effect on the oxidative stability of olive oil varieties grown in the southwest of Spain
Franco, M. N., Díaz, T. G., Sánchez, J., Miguel, C. D., & Martín‐Vertedor, D. (2014) · Grasas y Aceites · doi:10.3989/gya.051513 · Cited by 25
Molecular Phylogenetics and Biological Potential of Fungal Endophytes From Plants of the Sundarbans Mangrove
Rahaman, M. S., Siraj, M. A., Sultana, S., Seidel, V., & Islam, M. A. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.570855 · Cited by 26
Chemical composition and antioxidant activity of the essential oil ofThymbra capitata(L.) Cav. in Spain
Salas, J. B., Téllez, T. R., Pérez‐Alonso, M. J., Pardo, F. M. V., Capdevila, M. D. L. Á. C., & Rodríguez, C. G. (2010) · Acta Botanica Gallica · doi:10.1080/12538078.2010.10516189 · Cited by 24
Differences in antioxidant levels of fresh, frozen and freeze-dried strawberries and strawberry jam
Marques, K. K., Renfroe, M. H., Brevard, P. B., Lee, R. E., & Gloeckner, J. W. (2010) · International Journal of Food Sciences and Nutrition · doi:10.3109/09637481003796306 · Cited by 24
Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip
Krylov, A. V., Adamzig, H., Walter, A. D., Löchel, B., Kurth, E., Pulz, O., Szeponik, J., Wegerich, F., & Lisdat, F. (2006) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2005.11.062 · Cited by 24
Antioxidant Activity of Elderberry Fruits during Maturation
Imenšek, N., Kristl, J., Šumenjak, T. K., & Ivančič, A. (2021) · Agriculture · doi:10.3390/agriculture11060555 · Cited by 23
Application of experimental design and response surface methodology to optimize the procedure to obtain a bactericide and highly antioxidant aqueous extract from orange peels
González‐Gómez, D., Cardoso, V., Bohoyo, D., Ayuso, M. C., & Delgado, J. (2013) · Food Control · doi:10.1016/j.foodcont.2013.07.013 · Cited by 23
Assessing the activity of natural food antioxidants
Nenadis, N., & Tsimidou, M. Z. (2010) · Elsevier eBooks · doi:10.1533/9780857090447.2.332 · Cited by 23
Sensors Based on Metal Nanoclusters Stabilized on Designed Proteins
Aires, A., López‐Martínez, E., & Cortajarena, A. L. (2018) · Biosensors · doi:10.3390/bios8040110 · Cited by 22
Quantificação de metabólitos secundários e avaliação da atividade antimicrobiana e antioxidante de algumas plantas selecionadas do Cerrado de Mato Grosso
NETO, G. A. L., KAFFASHI, S., Luiz, W., FERREIRA, W. R., SILVA, Y. S. A. D. D., PAZIN, G. V., & Violante, I. M. P. (2015) · Revista Brasileira de Plantas Medicinais · doi:10.1590/1983-084x/14_161 · Cited by 22
Method to Improve Azo-Compound (AAPH)-Induced Hemolysis of Erythrocytes for Assessing Antioxidant Activity of Lipophilic Compounds
Nuruki, Y., Matsumoto, H., Tsukada, M., Tsukahara, H., Takajo, T., Tsuchida, K., & Anzai, K. (2020) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.c20-00568 · Cited by 20
Natural products of cosmetics: Analysis of extracts of plants endemic to Taiwan for the presence of tyrosinase-inhibitory, melanin-reducing, and free radical scavenging activities
Jiang, C.-B., Chang, M.-J., Wen, C. L., Lin, Y., Hsu, F.-L., & Lee, M.-H. (2020) · Journal of Food and Drug Analysis · doi:10.38212/2224-6614.2451 · Cited by 20
Ultrasound-Assisted Deep Eutectic Solvent Extraction of Antioxidant and Anti-Colorectal Cancer Proteins from Spirulina Biomass: Process Intensification, Characterization, and Bioactivity Evaluation
Zin, M. T., Kaewkod, T., Chaipoot, S., Kanthakat, G., Chen, Y.-Y., Cheirsilp, B., & Srinuanpan, S. (2025) · Antioxidants · doi:10.3390/antiox14030365 · Cited by 21
Influence of different extraction methods on the compound profiles and functional properties of extracts from solid by-products of the wine industry
Casquete, R., Benito, M. J., Martı́n, A., Martínez, A. T., Rivas, M. D. L. Á., & Córdoba, M. D. G. (2022) · LWT · doi:10.1016/j.lwt.2022.114097 · Cited by 19
Micro‐encapsulation of refined olive oil: influence of capsule wall components and the addition of antioxidant additives on the shelf life and chemical alteration
Calvo, P., Núñez, Á. L. C., Lozano, M., & González‐Gómez, D. (2012) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.5689 · Cited by 19
Optimization, extraction, structure analysis and antioxidant properties of flavan-3-ol polymers: Proanthocyanidins isolated from Periploca angustifolia using surface response methodology
Diwani, N., Fakhfakh, J., Athmouni, K., Belhaj, D., Feki, A. E., Allouche, N., Ayadi, H., & Bouaziz‐Ketata, H. (2019) · Industrial Crops and Products · doi:10.1016/j.indcrop.2019.112040 · Cited by 19
Pakistolides A and B, Novel Enzyme Inhibitory and Antioxidant Dimeric 4‐(Glucosyloxy)Benzoates from Berchemia pakistanica
Mukhtar, N., Malik, A. M., Riaz, N., Iqbal, K., Tareen, R. B., Khan, S. N., Nawaz, S. A., Siddiqui, J., & Choudhary, M. I. (2004) · Helvetica Chimica Acta · doi:10.1002/hlca.200490040 · Cited by 19
ABTS/TEAC (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)/Trolox®-Equivalent Antioxidant Capacity) radical scavenging mixed-mode assay
Cano, A., & Arnao, M. B. (2017) · Wiley eBooks · doi:10.1002/9781119135388.ch7 · Cited by 18
Self-citation
Antioxidant Activity, Total Phenolic and Flavonoid Contents in Floral Saffron Bio-Residues
Maestre-Hernández, A.-B., Vicente-López, J.-J., Pérez‐Llamas, F., Candela-Castillo, M.-E., García‐Conesa, M., Fernández, M. J. F., Cano, A., Hernández‐Ruíz, J., & Arnao, M. B. (2023) · Processes · doi:10.3390/pr11051400 · Cited by 18
Self-citation
Comparative Analysis of Antioxidant Activities of Fourteen Mentha Essential Oils and Their Components
Sitzmann, J., Habegger, R., Schnitzler, W. H., & Graßmann, J. (2014) · Chemistry & Biodiversity · doi:10.1002/cbdv.201400100 · Cited by 18
Ethyl Gallate Isolated from Castanopsis cuspidata var. sieboldii Branches Inhibits Melanogenesis and Promotes Autophagy in B16F10 Cells
Choi, M., Yang, S.-H., Kim, D., Kim, N. D., & Shin, H. (2023) · Antioxidants · doi:10.3390/antiox12020269 · Cited by 17
The peroxidase-like activity of Au NPs deposited inverse opal CeO2 nanozyme for rapid and sensitive H2O2 sensing
Jabiyeva, N., Çakıroğlu, B., & Özdemir, A. (2024) · Journal of Photochemistry and Photobiology A Chemistry · doi:10.1016/j.jphotochem.2024.115576 · Cited by 17
An integrated approach for assessing the in vitro and in vivo redox-related effects of nanomaterials
Vardakas, P., Skaperda, Z., Tekos, F., Trompeta, A.-F. A., Tsatsakis, A. M., Charitidis, C. A., & Kouretas, D. (2021) · Environmental Research · doi:10.1016/j.envres.2021.111083 · Cited by 20
Formulation and Evaluation of the Antioxidant Activity of an Emulsion Containing a Commercial Green Tea Extract
Sykuła, A., Janiak-Włodarczyk, I., & Kapusta, I. T. (2025) · Molecules · doi:10.3390/molecules30010197 · Cited by 16
Tunisian Wild Olive Leaves: Phenolic Compounds and Antioxidant Activity as an Important Step Toward Their Valorization
Baccouri, B., Mechi, D., Rajhi, I., & Martín‐Vertedor, D. (2022) · Food Analytical Methods · doi:10.1007/s12161-022-02430-z · Cited by 16
Biomass-Derived Plant Extracts in Macromolecular Chitosan Matrices as a Green Coating for PLA Films
Zemljič, L. F., Glaser, T. K., Plohl, O., Anžel, I., Šimat, V., Čagalj, M., Mežnar, E., Malin, V., Sterniša, M., & Možina, S. S. (2022) · Journal of Functional Biomaterials · doi:10.3390/jfb13040228 · Cited by 15
Antioxidant polysaccharide/gelatin blend films loaded with curcumin — A comparative study
Łupina, K., Kowalczyk, D., Lis, M., & Basiura, M. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.123945 · Cited by 15
Cooking does not decrease hydrophilic antioxidant capacity of wild blueberries
Murphy, R. R., Renfroe, M. H., Brevard, P. B., Lee, R. E., & Gloeckner, J. W. (2009) · International Journal of Food Sciences and Nutrition · doi:10.1080/09637480802495297 · Cited by 14
Combined Foliar Zinc and Nitrogen Application in Broccoli (Brassica oleracea var. italica L.): Effects on Growth, Nutrient Bioaccumulation, and Bioactive Compounds
Rivera-Martín, A. M., Reynolds-Marzal, D., Martı́n, A., Velázquez, R., & Poblaciones, M. J. (2021) · Agronomy · doi:10.3390/agronomy11030548 · Cited by 14
Hydromethanolic leaves extract of Dalbergia sissoo Roxb. ex DC. induces apoptosis in lung adenocarcinoma cells
Thakkar, A. B., Subramanian, R. B., Thakkar, V. R., & Thakor, P. (2023) · Process Biochemistry · doi:10.1016/j.procbio.2023.10.006 · Cited by 13

Authors: Cano, A., Acosta, M., & Arnao, M. B.
Affiliations: Universidad de Murcia (ES)
ORCID: Cano, A.
Acosta, M.
Arnao, M.B.
Venue: Redox Report
Publisher: Informa UK Limited
Published: 2000-12
Volume: 5
Issue: 6
Pages: 365-370
ISSN: 1351-0002 (ISSN-L)
1743-2928
DOI: 10.1179/135100000101535933
OpenAlex: W2004853818
Cited by: 156
References: 24
Citations by year: 2026: 1 · 2025: 7 · 2024: 11 · 2023: 12 · 2022: 10 · 2021: 13 · 2020: 14 · 2019: 5 · 2018: 3 · 2017: 7 · 2016: 5 · 2015: 9 · 2014: 6 · 2013: 8 · 2012: 6
FWCI: 1.37
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Redox Report (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
Keywords: antioxidant activity, ABTS radical, organic media, lipophilic vitamins, alpha-tocopherol, beta-carotene, Trolox, horseradish peroxidase, hydrogen peroxide, spectrophotometric assay, detection limit
MeSH terms: Ethanol, Antioxidants, Calibration, Chromans, Free Radicals, Horseradish Peroxidase, Indicators and Reagents, Sensitivity and Specificity, Solvents, Spectrophotometry, Sulfonic Acids, Vitamin E, Vitamins, Reproducibility of Results, beta Carotene, Benzothiazoles

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A method to measure antioxidant activity in organic media: application to lipophilic vitamins

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks