The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Complete reconstitution of human lymphocytes from cord blood CD34+ cells using the NOD/SCID/γcnull mice model
Open accessAbstract
Establishment of an assay capable of generating all classes of human lymphocytes from hematopoietic stem cells (HSCs) will provide new insight into the mechanism of human lymphopoiesis. We report ontogenic, functional, and histologic examination results of reconstituted human lymphocytes in NOD/SCID/ γcnull mice after the transplantation of human cord blood (CB) CD34+ cells. After transplantation, human B, natural killer (NK), and T cells were invariably identified in these mice, even though no human tissues were cotransplanted. Immature B cells resided mainly in bone marrow (BM), whereas mature B cells with surface immunoglobulins were preferentially found in spleen. NK cells were identified in BM and spleen. T cells were observed in various lymphoid organs, but serial examinations after transplantation confirmed human T lymphopoiesis occurring in the thymus. These human lymphocytes were also functionally competent. Human immunoglobulin M (IgM), IgA, and IgG were detected in the sera of these mice. T cells showed a diverse repertoire of T-cell–receptor Vβ (TCR Vβ) chains, proliferated in response to phytohemagglutinin, and were cytotoxic against cell lines. NK activity was demonstrated using the K562 cell line. Immunohistochemical analysis revealed that human lymphocytes formed organized structures in spleen and thymus that were analogous to those seen in humans. In the thymus, CD4 and CD8 double-positive T cells were predominant and coexpressed CD1a and Ki-67, thereby supporting the notion that T lymphopoiesis was taking place. NOD/SCID/ γcnull mice provide a unique model to investigate human lymphopoiesis without the cotransplantation of human tissues.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
NOD/SCID/γcnull mouse: an excellent recipient mouse model for engraftment of human cells
Ito, M., Hiramatsu, H., Kobayashi, K., Suzue, K., Kawahata, M., Hioki, K., Ueyama, Y., Koyanagi, Y., Sugamura, K., Tsuji, K., et al. (2002) · Blood · doi:10.1182/blood-2001-12-0207 · Cited by 1,476
Self-citationMcCune, J., Namikawa, R., Kaneshima, H., Shultz, L., Lieberman, M., & Weissman, I. (1988) · Science · doi:10.1126/science.241.4873.1632 · Cited by 1,406
Noguchi, M. (1993) · Cell · doi:10.1016/0092-8674(93)90167-o · Cited by 1,320
Transfer of a functional human immune system to mice with severe combined immunodeficiency
Mosier, D. E., Gulizia, R. J., Baird, S. M., & Wilson, D. B. (1988) · Nature · doi:10.1038/335256a0 · Cited by 1,255
Cytokine Stimulation of Multilineage Hematopoiesis from Immature Human Cells Engrafted in SCID Mice
Lapidot, T., Pflumio, F., Doedens, M., Murdoch, B., Williams, D. E., & Dick, J. E. (1992) · Science · doi:10.1126/science.1372131 · Cited by 535
In vivo evidence for a dependence on interleukin 15 for survival of natural killer cells
Cooper, M. A., Bush, J. E., Fehniger, T. A., VanDeusen, J. B., Waite, R. E., Liu, Y., Aguila, H. L., & Caligiuri, M. A. (2002) · Blood · doi:10.1182/blood-2001-12-0293 · Cited by 455
Long-term human hematopoiesis in the SCID-hu mouse.
Namikawa, R., Weilbaecher, K. N., Kaneshima, H., Yee, E. J., & McCune, J. M. (1990) · The Journal of experimental medicine · doi:10.1084/jem.172.4.1055 · Cited by 333
Puck, J. M., Deschenes, S. M., Porter, J. C., Dutra, A. S., Brown, C. J., Willard, H. F., & Henthorn, P. S. (1993) · Human Molecular Genetics · doi:10.1093/hmg/2.8.1099 · Cited by 305
Ueda, T., Tsuji, K., Yoshino, H., Ebihara, Y., Yagasaki, H., Hisakawa, H., Mitsui, T., Manabe, A., Tanaka, R., Kobayashi, K., et al. (2000) · Journal of Clinical Investigation · doi:10.1172/jci8583 · Cited by 280
Self-citationLichtenfels, R., Biddison, W. E., Schulz, H., Vogt, A. B., & Martin, R. (1994) · Journal of Immunological Methods · doi:10.1016/0022-1759(94)90110-4 · Cited by 263
Cited by
Miyara, M., Yoshioka, Y., Kitoh, A., Shima, T., Wing, K., Niwa, A., Parizot, C., Taflin, C., Heike, T., Valeyre, D., et al. (2009) · Immunity · doi:10.1016/j.immuni.2009.03.019 · Cited by 2,291
Self-citationTackling the cancer stem cells — what challenges do they pose?
Pattabiraman, D. R., & Weinberg, R. A. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4253 · Cited by 990
Current advances in humanized mouse models
Ito, R., Takahashi, T., Katano, I., & Ito, M. (2012) · Cellular & Molecular Immunology · doi:10.1038/cmi.2012.2 · Cited by 349
Self-citationAn animal model of adult T-cell leukemia: humanized mice with HTLV-1–specific immunity
Tezuka, K., Xun, R., Tei, M., Ueno, T., Tanaka, M., Takenouchi, N., & Fujisawa, J.-I. (2014) · Blood · doi:10.1182/blood-2013-06-508861 · Cited by 68
HTLV-1 Propels Thymic Human T Cell Development in “Human Immune System” Rag2-/- gamma c-/- Mice
Villaudy, J., Wencker, M., Gadot, N., Gillet, N. A., Scoazec, J.-Y., Gazzolo, L., Manz, M. G., Bangham, C. R. M., & Dodon, M. D. (2011) · PLoS Pathogens · doi:10.1371/journal.ppat.1002231 · Cited by 66
Metadata
- Authors
- Hiramatsu, H., Nishikomori, R., Heike, T., Ito, M., Kobayashi, K., Katamura, K., & Nakahata, T.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2003-08-01
- Volume
- 102
- Issue
- 3
- Pages
- 873-880
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2024446294
- Cited by
- 254
- References
- 26
- FWCI
- 7.10
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Complete reconstitution of human lymphocytes from cord blood CD34+ cells using the NOD/SCID/γcnull mice model. https://app.cochat.ai/papers/10.1182/blood-2002-09-2755
- The paper
- Hiramatsu, H., Nishikomori, R., Heike, T., Ito, M., Kobayashi, K., Katamura, K., & Nakahata, T. (2003). Complete reconstitution of human lymphocytes from cord blood CD34+ cells using the NOD/SCID/γcnull mice model. Blood. https://doi.org/10.1182/blood-2002-09-2755
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
