The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.
T-cell receptor–induced phosphorylation of the ζ chain is efficiently promoted by ZAP-70 but not Syk
Abstract
Engagement of the T-cell receptor (TCR) results in the activation of Lck/Fyn and ZAP-70/Syk tyrosine kinases. Lck-mediated tyrosine phosphorylation of signaling motifs (ITAMs) in the CD3-ζ subunits of the TCR is an initial step in the transduction of signaling cascades. However, ζ phosphorylation is also promoted by ZAP-70, as TCR-induced ζ phosphorylation is defective in ZAP-70–deficient T cells. We show that this defect is corrected by stable expression of ZAP-70, but not Syk, in primary and transformed T cells. Indeed, these proteins are differentially coupled to the TCR with a 5- to 10-fold higher association of ZAP-70 with ζ as compared to Syk. Low-level Syk-ζ binding is associated with significantly less Lck coupled to the TCR. Moreover, diminished coupling of Lck to ζ correlates with a poor phosphorylation of the positive regulatory tyr352 residue of Syk. Thus, recruitment of Lck into the TCR complex with subsequent ζ chain phosphorylation is promoted by ZAP-70 but not Syk. Importantly, the presence of ZAP-70 positively regulates the TCR-induced tyrosine phosphorylation of Syk. The interplay between Syk and ZAP-70 in thymocytes, certain T cells, and B-chronic lymphocytic leukemia cells, in which they are coexpressed, will therefore modulate the amplitude of antigen-mediated receptor signaling.
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“ZAP-70 has been reported to promote the phosphorylation of CD3ζ and the association of CD3ζ with Lck, as ZAP-70 deficiency impairs the TCR-induced CD3ζ phosphorylation[8], [9], [10].”
From RhoH Regulates Subcellular Localization of ZAP-70 and Lck in T Cell Receptor Signaling (2010) · cites this paper as [9]
In the citing paper
“In addition, ZAP-70 has been reported to promote CD3ζ phosphorylation by recruiting Lck to TCR complex in a kinase activity-independent manner[9].”
From RhoH Regulates Subcellular Localization of ZAP-70 and Lck in T Cell Receptor Signaling (2010) · cites this paper as [9]
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ZAP-70: A 70 kd protein-tyrosine kinase that associates with the TCR ζ chain
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Complex Complexes: Signaling at the TCR
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Research works printed but not linked: 2
- Chen L, Lang H, Dicker F, et al. Demonstration that ZAP-70 directly contributes to enhanced B cell receptor signaling in chronic lymphocytic leukemia [abstract]. Blood. 2003;102: 34.
- Di Bartolo V, Malissen M, Dufour E, Sechet E, Malissen B, Acuto O. Tyrosine 315 determines optimal recruitment of ZAP-70 to the T-cell antigen receptor. Eur J Immunol. 2002;32: 568-575. · DOI
Cited by
Oxyl radicals, redox-sensitive signalling cascades and antioxidants
Genestra, M. (2007) · Cellular Signalling · doi:10.1016/j.cellsig.2007.04.009 · Cited by 4,930
Alternative p38 activation pathway mediated by T cell receptor-proximal tyrosine kinases
Salvador, J. M., Mittelstadt, P. R., Guszczynski, T., Copeland, T. D., Yamaguchi, H., Appella, E., Fornace, A. J., & Ashwell, J. D. (2005) · Nature Immunology · doi:10.1038/ni1177 · Cited by 306
RhoH Regulates Subcellular Localization of ZAP-70 and Lck in T Cell Receptor Signaling
Chae, H.-D., Siefring, J. E., Hildeman, D. A., Gu, Y., & Williams, D. A. (2010) · PLoS ONE · doi:10.1371/journal.pone.0013970 · Cited by 35
Metadata
- Authors
- Steinberg, M., Adjali, O., Swainson, L., Merida, P., Bartolo, V. D., Pelletier, L., Taylor, N., & Noraz, N.
- Affiliations
- From the Institut de Génétique Moléculaire de Montpellier, Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherches (UMR) 5535/Institut Fédératif de Recherche (IFR) 122, F-34293 Montpellier Cedex 5; and the Laboratory of Molecular Immunology, Institut Pasteur, 75724 Paris Cedex 15, France.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2004-08-01
- Volume
- 104
- Issue
- 3
- Pages
- 760-767
- ISSN
- 0006-4971
- 1528-0020
- Cited by
- 22
- References
- 71
- Language
- en
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- Steinberg, M., Adjali, O., Swainson, L., Merida, P., Bartolo, V. D., Pelletier, L., Taylor, N., & Noraz, N. (2004). T-cell receptor–induced phosphorylation of the ζ chain is efficiently promoted by ZAP-70 but not Syk. Blood. https://doi.org/10.1182/blood-2003-12-4314
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