The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Murine embryonic stem cell differentiation is promoted by SOCS-3 and inhibited by the zinc finger transcription factor Klf4
Abstract
Embryonic stem (ES) cells homozygous for a Shp-2 mutation (Shp-2Δ46-110) demonstrate leukemia inhibitory factor (LIF) hypersensitivity and increased LIF-stimulated phosphorylation of signal transducer and activator of transcription (STAT3). We hypothesized that LIF-responsive genes in Shp-2Δ46-110 cells would represent potential candidates for molecules vital for ES cell self-renewal. Using microarray analysis, we detected 41 genes whose expression was modified by LIF in Shp-2Δ46-110 ES cells. Induction of 2 significantly up-regulated genes, suppressor of cytokine signaling–3 (SOCS-3) and Krüppel-like factor 4 (Klf4), was verified using Northern blotting. ES cells overexpressing SOCS-3 had an increased capacity to differentiate to hematopoietic progenitors, rather than to self-renew. In contrast, ES cells overexpressing Klf4 had a greater capacity to self-renew based on secondary embryoid body (EB) formation. Klf4-transduced d6 EBs expressed higher levels of Oct-4, consistent with the notion that Klf4 promotes ES cell self-renewal. These findings verify the negative role of SOCS-3 on LIF signaling and provide a novel role for Klf4 in ES cell function.
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Nicholson, S. E., Willson, T. A., Farley, A., Starr, R., Zhang, J., Baca, M., Alexander, W. S., Metcalf, D., Hilton, D. J., & Nicola, N. A. (1999) · The EMBO Journal · doi:10.1093/emboj/18.2.375 · Cited by 407
Auernhammer, C. J., Bousquet, C., & Melmed, S. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.12.6964 · Cited by 284
Cited by
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Generation of germline-competent induced pluripotent stem cells
Okita, K., Ichisaka, T., & Yamanaka, S. (2007) · Nature · doi:10.1038/nature05934 · Cited by 4,299
Mammalian Krüppel-Like Factors in Health and Diseases
McConnell, B. B., & Yang, V. W. (2010) · Physiological Reviews · doi:10.1152/physrev.00058.2009 · Cited by 1,018
A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells
Niwa, H., Ogawa, K., Shimosato, D., & Adachi, K. (2009) · Nature · doi:10.1038/nature08113 · Cited by 872
How is pluripotency determined and maintained?
Niwa, H. (2007) · Development · doi:10.1242/dev.02787 · Cited by 783
Metadata
- Authors
- Li, Y., McClintick, J., Zhong, L., Edenberg, H. J., Yoder, M. C., & Chan, R. J.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2005-01-15
- Volume
- 105
- Issue
- 2
- Pages
- 635-637
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2088861927
- Cited by
- 292
- References
- 2
- FWCI
- 2.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- Li, Y., McClintick, J., Zhong, L., Edenberg, H. J., Yoder, M. C., & Chan, R. J. (2005). Murine embryonic stem cell differentiation is promoted by SOCS-3 and inhibited by the zinc finger transcription factor Klf4. Blood. https://doi.org/10.1182/blood-2004-07-2681
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