CoChat
Sign Up
Cited by 36

The DOI matches its Crossref record.

Reduced activation of protein kinase B, Rac, and F-actin polymerization contributes to an impairment of stromal cell derived factor-1 induced migration of CD34+ cells from patients with myelodysplasia

Fuhler, Gwenny M., Drayer, A. Lyndsay, Olthof, Sandra G. M., Schuringa, Jan Jacob, Coffer, Paul J., Vellenga, Edo·2008·Blood
DOI 10.1182/blood-2006-11-060632
Open this paper in CoChat — free account

Abstract

Patients with myelodysplasia (MDS) show a differentiation defect in the multipotent stem-cell compartment. An important factor in stem-cell differentiation is their proper localization within the bone marrow microenvironment, which is regulated by stromal cell–derived factor (SDF-1). We now show that SDF-1–induced migration of CD34+ progenitor cells from MDS patients is severely impaired. In addition, these cells show a reduced capacity to polymerize F-actin in response to SDF-1. We demonstrate a major role for Rac and phosphatidylinositol 3-kinase (PI3K) and a minor role for the extracellular signal-regulated kinase (ERK)1/2 signaling pathway in SDF-1–induced migration of normal CD34+ cells. Furthermore, SDF-1–stimulated activation of Rac and the PI3K target protein kinase B is impaired in CD34+ cells from MDS patients. Lentiviral transduction of MDS CD34+ cells with constitutive active Rac1V12 results in a partial restoration of F-actin polymerization in response to SDF-1. In addition, expression of constitutive active Rac increases the motility of MDS CD34+ cells in the absence of SDF-1, although the directional migration of cells toward this chemoattractant is not affected. Taken together, our results show a reduced migration of MDS CD34+ cells toward SDF-1, as a result of impaired activation of the PI3K and Rac pathways and a decreased F-actin polymerization.

Overview

Integrity signals

Registry

Crossref · 10.1182/blood-2006-11-060632

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 51

  1. Houwerzijl, 2004, Increased peripheral platelet destruction and caspase-3-independent programmed cell death of bone marrow megakaryoctes in myelodysplastic patients., Blood, 103, 500, 10.1182/blood-2003-01-0275 · DOI
  2. Fuhler, 2003, Decreased phosphorylation of protein kinase B (PKB/Akt) and extracellular signal-regulated kinase (ERK) in neutrophils from patients with myelodysplasia., Blood, 101, 1172, 10.1182/blood.V101.3.1172 · DOI
  3. Fuhler, 2005, Impaired interleukin-8- and GROalpha-induced phosphorylation of extracellular signal-regulated kinase result in decreased migration of neutrophils from patients with myelodysplasia., J Leukoc Biol, 77, 257, 10.1189/jlb.0504306 · DOI
  4. Zabernigg, 1997, Cytokine priming of the granulocyte respiratory burst in myelodysplastic syndromes., Leuk Lymphoma, 27, 137, 10.3109/10428199709068280 · DOI
  5. Ohsaka, 1993, Effects of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor on respiratory burst activity of neutrophils in patients with myelodysplastic syndromes., Clin Exp Immunol, 91, 308, 10.1111/j.1365-2249.1993.tb05900.x · DOI
  6. Suzuki, 2006, Combinatorial Gata2 and Sca1 expression defines hematopoietic stem cells in the bone marrow niche., Proc Natl Acad Sci U S A, 103, 2202, 10.1073/pnas.0508928103 · DOI
  7. Muguruma, 2006, Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment., Blood, 107, 1878, 10.1182/blood-2005-06-2211 · DOI
  8. Spradling, 2001, Stem cells find their niche., Nature, 414, 98, 10.1038/35102160 · DOI
  9. Avecilla, 2004, Chemokine-mediated interaction of hematopoietic progenitors with the bone marrow vascular niche is required for thrombopoiesis., Nat Med, 10, 64, 10.1038/nm973 · DOI
  10. Aiuti, 1997, The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to peripheral blood., J Exp Med, 185, 111, 10.1084/jem.185.1.111 · DOI · Paper page
  11. Jones, 2000, Cellular signaling in macrophage migration and chemotaxis., J Leukoc Biol, 68, 593, 10.1189/jlb.68.5.593 · DOI
  12. Ridley, 2003, Cell migration: integrating signals from front to back., Science, 302, 1704, 10.1126/science.1092053 · DOI
  13. Postma, 2004, Chemotaxis: signalling modules join hands at front and tail., EMBO Rep, 5, 35, 10.1038/sj.embor.7400051 · DOI
  14. Voermans, 2001, SDF-1-induced actin polymerization and migration in human hematopoietic progenitor cells., Exp Hematol, 29, 1456, 10.1016/S0301-472X(01)00740-8 · DOI
  15. Bug, 2002, Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells., J Leukoc Biol, 72, 837, 10.1189/jlb.72.4.837 · DOI
  16. Coffer, 1998, Comparison of the roles of mitogen-activated protein kinase kinase and phosphatidylinositol 3-kinase signal transduction in neutrophil effector function., Biochem J, 329, 121, 10.1042/bj3290121 · DOI
  17. Servant, 2000, Polarization of chemoattractant receptor signaling during neutrophil chemotaxis., Science, 287, 1037, 10.1126/science.287.5455.1037 · DOI
  18. Petit, 2005, Atypical PKC-zeta regulates SDF-1-mediated migration and development of human CD34+ progenitor cells., J Clin Invest, 115, 168, 10.1172/JCI200521773 · DOI
  19. Lehman, 2001, MAP kinase upregulation after hematopoietic differentiation: role of chemotaxis., Am J Physiol Cell Physiol, 280, C183, 10.1152/ajpcell.2001.280.1.C183 · DOI
  20. Hii, 1999, Role of the extracellular signal-regulated protein kinase cascade in human neutrophil killing of Staphylococcus aureus and Candida albicans and in migration., Infect Immun, 67, 1297, 10.1128/IAI.67.3.1297-1302.1999 · DOI
  21. Brahmbhatt, 2003, ERK and RhoA differentially regulate pseudopodia growth and retraction during chemotaxis., J Biol Chem, 278, 13016, 10.1074/jbc.M211873200 · DOI
  22. Wang, 2000, Stromal cell-derived factor-1alpha stimulates tyrosine phosphorylation of multiple focal adhesion proteins and induces migration of hematopoietic progenitor cells: roles of phosphoinositide-3 kinase and protein kinase C., Blood, 95, 2505, 10.1182/blood.V95.8.2505 · DOI
  23. Bennett, 1982, Proposals for the classification of the myelodysplastic syndromes., Br J Haematol, 51, 189, 10.1111/j.1365-2141.1982.tb08475.x · DOI
  24. Nijhuis, 2002, Src kinases regulate PKB activation and modulate cytokine and chemoattractant-controlled neutrophil functioning., J Leukoc Biol, 71, 115, 10.1189/jlb.71.1.115 · DOI
  25. Sander, 1998, Matrix-dependent Tiam1/Rac signaling in epithelial cells promotes either cell-cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase., J Cell Biol, 143, 1385, 10.1083/jcb.143.5.1385 · DOI
  26. Fischer, 2004, Regulation of CXC chemokine receptor 4-mediated migration by the Tec family tyrosine kinase ITK., J Biol Chem, 279, 29816, 10.1074/jbc.M312848200 · DOI
  27. Sato, 2004, Involvement of 3-phosphoinositide-dependent protein kinase-1 in the MEK/MAPK signal transduction pathway., J Biol Chem, 279, 33759, 10.1074/jbc.M402055200 · DOI
  28. Knall, 1997, Interleukin 8-stimulated phosphatidylinositol-3-kinase activity regulates the migration of human neutrophils independent of extracellular signal-regulated kinase and p38 mitogen-activated protein kinases., Proc Natl Acad Sci U S A, 94, 3052, 10.1073/pnas.94.7.3052 · DOI
  29. Roberts, 1999, Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is characterized by abnormalities in neutrophil function and host defense., Immunity, 10, 183, 10.1016/S1074-7613(00)80019-9 · DOI · Paper page
  30. Coles, 2002, PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway., Oncogene, 21, 2236, 10.1038/sj.onc.1205302 · DOI
  31. Innocenti, 2003, Phosphoinositide 3-kinase activates Rac by entering in a complex with Eps8, Abi1, and Sos-1., J Cell Biol, 160, 17, 10.1083/jcb.200206079 · DOI
  32. Srinivasan, 2003, Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis., J Cell Biol, 160, 375, 10.1083/jcb.200208179 · DOI
  33. Dar, 2006, Mutual, reciprocal SDF-1/CXCR4 interactions between hematopoietic and bone marrow stromal cells regulate human stem cell migration and development in NOD/SCID chimeric mice., Exp Hematol, 34, 967, 10.1016/j.exphem.2006.04.002 · DOI
  34. Peled, 1999, Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4., Science, 283, 845, 10.1126/science.283.5403.845 · DOI · Paper page
  35. Chatta, 1993, Hematopoietic progenitors and aging: alterations in granulocytic precursors and responsiveness to recombinant human G-CSF, GM-CSF, and IL-3., J Gerontol, 48, M207, 10.1093/geronj/48.5.M207 · DOI
  36. Hall, 2005, Rho GTPases and the control of cell behaviour., Biochem Soc Trans, 33, 891, 10.1042/BST0330891 · DOI
  37. Cancelas, 2006, The role of chemokine activation of Rac GTPases in hematopoietic stem cell marrow homing, retention, and peripheral mobilization., Exp Hematol, 34, 976, 10.1016/j.exphem.2006.03.016 · DOI
  38. Ghiaur, 2006, Inhibition of RhoA GTPase activity enhances hematopoietic stem and progenitor cell proliferation and engraftment., Blood, 108, 2087, 10.1182/blood-2006-02-001560 · DOI
  39. Rozenveld-Geugien, 2007, Expansion of normal and leukemic human hematopoietic stam/progenitor cells requires Rac-mediated interaction with stromal cells., Exp Hematol, 35, 782, 10.1016/j.exphem.2007.02.006 · DOI · Paper page
  40. Ridley, 2001, Rho proteins: linking signaling with membrane trafficking., Traffic, 2, 303, 10.1034/j.1600-0854.2001.002005303.x · DOI
  41. Bakin, 2000, Phosphatidylinositol 3-kinase function is required for transforming growth factor beta-mediated epithelial to mesenchymal transition and cell migration., J Biol Chem, 275, 36803, 10.1074/jbc.M005912200 · DOI
  42. Li, 2005, Role of guanine nucleotide exchange factor P-Rex-2b in sphingosine 1-phosphate-induced Rac1 activation and cell migration in endothelial cells., Prostaglandins Other Lipid Mediat, 76, 95, 10.1016/j.prostaglandins.2005.02.002 · DOI
  43. Chen, 2003, Two phases of actin polymerization display different dependencies on PI(3,4,5)P3 accumulation and have unique roles during chemotaxis., Mol Biol Cell, 14, 5028, 10.1091/mbc.e03-05-0339 · DOI
  44. Arai, 2006, CrkL plays a role in SDF-1-induced activation of the Raf-1/MEK/Erk pathway through Ras and Rac to mediate chemotactic signaling in hematopoietic cells., Cell Signal, 18, 2162, 10.1016/j.cellsig.2006.05.001 · DOI
  45. Gu, 2005, RhoH, a hematopoietic-specific Rho GTPase, regulates proliferation, survival, migration, and engraftment of hematopoietic progenitor cells., Blood, 105, 1467, 10.1182/blood-2004-04-1604 · DOI · Paper page
  46. Onai, 2000, Impairment of lymphopoiesis and myelopoiesis in mice reconstituted with bone marrow-hematopoietic progenitor cells expressing SDF-1-intrakine., Blood, 96, 2074, 10.1182/blood.V96.6.2074 · DOI
  47. Fuhler, 2007, The reduced GM-CSF priming of ROS production in granulocytes from patients with myelodysplasia is associated with an impaired lipid raft formation., J Leukoc Biol, 81, 449, 10.1189/jlb.0506311 · DOI
  48. Gómez-Moutón, 2004, Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis., J Cell Biol, 164, 759, 10.1083/jcb.200309101 · DOI
  49. Giebel, 2004, Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells., Blood, 104, 2332, 10.1182/blood-2004-02-0511 · DOI
  50. van Buul, 2003, Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4., J Biol Chem, 278, 30302, 10.1074/jbc.M304764200 · DOI
  51. Wysoczynski, 2005, Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient., Blood, 105, 40, 10.1182/blood-2004-04-1430 · DOI

Cited by

Metadata

Authors
Fuhler, G. M., Drayer, A. L., Olthof, S. G. M., Schuringa, J. J., Coffer, P. J., & Vellenga, E.
Affiliations
  • Division of Hematology, Department of Medicine, University Medical Center Groningen, Groningen;
  • Sanquin Blood Bank North East Netherlands, Groningen; and
  • Department of Immunology, University Medical Center Utrecht, the Netherlands
Venue
Blood
Publisher
American Society of Hematology
Published
2008-01-01
Volume
111
Issue
1
Pages
359-368
ISSN
  • 0006-4971
  • 1528-0020
DOI
10.1182/blood-2006-11-060632
Cited by
36
References
0
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review