The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Runx1-mediated hematopoietic stem-cell emergence is controlled by a Gata/Ets/SCL-regulated enhancer
Open accessAbstract
The transcription factor Runx1/AML1 is an important regulator of hematopoiesis and is critically required for the generation of the first definitive hematopoietic stem cells (HSCs) in the major vasculature of the mouse embryo. As a pivotal factor in HSC ontogeny, its transcriptional regulation is of high interest but is largely undefined. In this study, we used a combination of comparative genomics and chromatin analysis to identify a highly conserved 531-bp enhancer located at position + 23.5 in the first intron of the 224-kb mouse Runx1 gene. We show that this enhancer contributes to the early hematopoietic expression of Runx1. Transcription factor binding in vivo and analysis of the mutated enhancer in transient transgenic mouse embryos implicate Gata2 and Ets proteins as critical factors for its function. We also show that the SCL/Lmo2/Ldb-1 complex is recruited to the enhancer in vivo. Importantly, transplantation experiments demonstrate that the intronic Runx1 enhancer targets all definitive HSCs in the mouse embryo, suggesting that it functions as a crucial cis-regulatory element that integrates the Gata, Ets, and SCL transcriptional networks to initiate HSC generation.
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Cites
LAGAN and Multi-LAGAN: Efficient tools for large-scale multiple alignment of genomic DNA
Brudno, M., Do, C. B., Cooper, G. M., Kim, M. F., Davydov, E., Program, N. C. S., Green, E. D., Sidow, A., & Batzoglou, S. (2003) · Genome Research · doi:10.1101/gr.926603 · Cited by 1,115
Cbfa2 is required for the formation of intra-aortic hematopoietic clusters
North, T., Gu, T.-L., Stacy, T., Wang, Q., Howard, L., Binder, M., Marín-Padilla, M., & Speck, N. A. (1999) · Development · doi:10.1242/dev.126.11.2563 · Cited by 662
Anguita, E., Hughes, J., Heyworth, C., Blobel, G. A., Wood, W. G., & Higgs, D. R. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600274 · Cited by 207
Genetic regulatory networks programming hematopoietic stem cells and erythroid lineage specification
Swiers, G., Patient, R., & Loose, M. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.02.051 · Cited by 168
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Kwiatkowski, N., Zhang, T., Rahl, P. B., Abraham, B. J., Reddy, J., Ficarro, S. B., Dastur, A., Amzallag, A., Ramaswamy, S., Tesar, B., et al. (2014) · Nature · doi:10.1038/nature13393 · Cited by 855
Wilson, N. K., Foster, S. D., Wang, X., Knezevic, K., Schütte, J., Kaimakis, P., Chilarska, P. M., Kinston, S., Ouwehand, W. H., Dzierzak, E., et al. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.07.016 · Cited by 728
Of lineage and legacy: the development of mammalian hematopoietic stem cells
Dzierzak, E., & Speck, N. A. (2008) · Nature Immunology · doi:10.1038/ni1560 · Cited by 707
cisTopic: cis-regulatory topic modeling on single-cell ATAC-seq data
Bravo González-Blas, C., Minnoye, L., Papasokrati, D., Aibar, S., Hulselmans, G., Christiaens, V., Davie, K., Wouters, J., & Aerts, S. (2019) · Nature Methods · doi:10.1038/s41592-019-0367-1 · Cited by 456
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Metadata
- Authors
- Nottingham, W. T., Jarratt, A., Burgess, M., Speck, C. L., Cheng, J.-F., Prabhakar, S., Rubin, E. M., Li, P.-S., Sloane-Stanley, J., Kong-a-San, J., & de Bruijn, M. F. T. R.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2007-12-15
- Volume
- 110
- Issue
- 13
- Pages
- 4188-4197
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2082425943
- Cited by
- 249
- References
- 4
- FWCI
- 7.57
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Nottingham, W. T., Jarratt, A., Burgess, M., Speck, C. L., Cheng, J.-F., Prabhakar, S., Rubin, E. M., Li, P.-S., Sloane-Stanley, J., Kong-a-San, J., & de Bruijn, M. F. T. R. (2007). Runx1-mediated hematopoietic stem-cell emergence is controlled by a Gata/Ets/SCL-regulated enhancer. Blood. https://doi.org/10.1182/blood-2007-07-100883
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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