Attack on the clones? Human FOXP3 detection by PCH101, 236A/E7, 206D, and 259D reveals 259D as the outlier with lower sensitivity

Seventeen-colour flow cytometry: unravelling the immune system

Perfetto, S. P., Chattopadhyay, P. K., & Roederer, M. (2004) · Nature Reviews Immunology · doi:10.1038/nri1416 · Cited by 1,019 · see citing works

Transient expression of FOXP3 in human activated nonregulatory CD4 ⁺ T cells

Wang, J., Ioan‐Facsinay, A., van der Voort, E. I. H., Huizinga, T. W. J., & Toes, R. E. M. (2007) · European Journal of Immunology · doi:10.1002/eji.200636435 · Cited by 1,011 · see citing works

Activation-induced FOXP3 in human T effector cells does not suppress proliferation or cytokine production

Allan, S. E., Crome, S. Q., Crellin, N. K., Passerini, L., Steiner, T. S., Bacchetta, R., Roncarolo, M. G., & Levings, M. K. (2007) · International Immunology · doi:10.1093/intimm/dxm014 · Cited by 872 · see citing works

Induction of FOXP3 expression in naive human CD4+FOXP3− T cells by T-cell receptor stimulation is transforming growth factor-β–dependent but does not confer a regulatory phenotype

Tran, D. Q., Ramsey, H. E., & Shevach, E. M. (2007) · Blood · doi:10.1182/blood-2007-06-094656 · Cited by 738 · see citing works

Interpreting flow cytometry data: a guide for the perplexed

Herzenberg, L. A., Tung, J., Moore, W. A., Herzenberg, L. A., & Parks, D. R. (2006) · Nature Immunology · doi:10.1038/ni0706-681 · Cited by 464 · see citing works

Transient regulatory T-cells: A state attained by all activated human T-cells

Pillai, V., Ortega, S. B., Wang, C. K., & Karandikar, N. J. (2006) · Clinical Immunology · doi:10.1016/j.clim.2006.10.014 · Cited by 339 · see citing works

Human regulatory T cells: A unique, stable thymic subset or a reversible peripheral state of differentiation?

Pillai, V., & Karandikar, N. J. (2007) · Immunology Letters · doi:10.1016/j.imlet.2007.08.012 · Cited by 47 · see citing works

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Cossarizza, A., Chang, H., Radbruch, A., Acs, A., Adam, D., Adam‐Klages, S., Agace, W. W., Aghaeepour, N., Akdiş, M., Allez, M., et al. (2019) · European Journal of Immunology · doi:10.1002/eji.201970107 · Cited by 1,000 · see citing works

Molecular Mechanisms of Treg-Mediated T Cell Suppression

Schmidt, A., Oberle, N., & Krammer, P. H. (2012) · Frontiers in Immunology · doi:10.3389/fimmu.2012.00051 · Cited by 760 · see citing works

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531 · see citing works

Regulatory T cells in inflammatory bowel disease

Boden, E. K., & Snapper, S. B. (2008) · Current Opinion in Gastroenterology · doi:10.1097/mog.0b013e328311f26e · Cited by 142 · see citing works

De Novo Generation and Enhanced Suppression of Human CD4+CD25+ Regulatory T Cells by Retinoic Acid

Wang, J., Huizinga, T. W. J., & Toes, R. E. M. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0901065 · Cited by 112 · see citing works

Phenotype and regulation of immunosuppressive Vδ2-expressing γδ T cells

Peters, C., Oberg, H., Kabelitz, D., & Wesch, D. (2013) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-013-1467-1 · Cited by 92 · see citing works

Acquisition of Suppressive Function by Activated Human CD4+CD25− T Cells Is Associated with the Expression of CTLA-4 Not FoxP3

Zheng, Y., Manzotti, C. N., Burke, F., Dussably, L., Qureshi, O., Walker, L. S. K., & Sansom, D. M. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.3.1683 · Cited by 86 · see citing works

The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4⁺CD25⁺Foxp3⁺ regulatory T cells

Law, J. P., Hirschkorn, D. F., Owen, R. E., Biswas, H. H., Norris, P. J., & Lanteri, M. C. (2009) · Cytometry Part A · doi:10.1002/cyto.a.20815 · Cited by 71 · see citing works

FoxP3 isoforms and PD-1 expression by T regulatory cells in multiple sclerosis

Sambucci, M., Gargano, F., Rosa, V. D., Bardi, M. D., Picozza, M., Placido, R., Ruggieri, S., Capone, A., Gasperini, C., Matarese, G., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-21861-5 · Cited by 72 · see citing works

Bronchial response pattern of antigen presenting cells and regulatory T cells in children less than 2 years of age

Heier, I., Malmstrom, K., Pelkonen, A. S., Malmberg, L. P., Kajosaari, M., Turpeinen, M., Lindahl, H. G., Brandtzaeg, P., Jahnsen, F. L., & Mäkelä, M. J. (2008) · Thorax · doi:10.1136/thx.2007.082974 · Cited by 52 · see citing works

Dexamethasone upregulates FOXP3 expression without increasing regulatory activity

Prado, C., Gómez, J. M. F., López, P., de Paz, B., Gutiérrez, C., & Suárez, A. (2010) · Immunobiology · doi:10.1016/j.imbio.2010.06.013 · Cited by 51 · see citing works

Homeostasis of peripheral FoxP3+ CD4+ regulatory T cells in patients with early and late stage breast cancer

Rech, A. J., Mick, R., Kaplan, D. E., Chang, K., Domchek, S. M., & Vonderheide, R. H. (2009) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-009-0780-x · Cited by 38 · see citing works

Bath-PUVA therapy induces circulating regulatory T cells in patients with psoriasis

Saito, C., Maeda, A., & Morita, A. (2008) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2008.09.011 · Cited by 36 · see citing works

A Network Modeling Approach to Analysis of the Th2 Memory Responses Underlying Human Atopic Disease

Bosco, A., McKenna, K., Firth, M. J., Sly, P. D., & Holt, P. G. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0804125 · Cited by 33 · see citing works

Prostaglandin E2 Affects T Cell Responses through Modulation of CD46 Expression

Kickler, K., Maltby, K., Choileáin, S. N., Stephen, J., Wright, S., Hafler, D. A., Jabbour, H. N., & Astier, A. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1103090 · Cited by 32 · see citing works

Regulatory T Cells in Colorectal Cancer: From Biology to Prognostic Relevance

Mougiakakos, D. (2011) · Cancers · doi:10.3390/cancers3021708 · Cited by 30 · see citing works

Association of two clones allows for optimal detection of human FOXP3

Presicce, P., Moreno‐Fernandez, M. E., Lages, C. S., Orsborn, K. I., & Chougnet, C. A. (2010) · Cytometry Part A · doi:10.1002/cyto.a.20875 · Cited by 30 · see citing works

Analysis of FOXP3+ regulatory T cell subpopulations in peripheral blood and tissue of patients with systemic lupus erythematosus

Schmidt, A., Rieger, C. C., Venigalla, R. K., Éliás, S., Max, R., Lorenz, H., Gröne, H., Krammer, P. H., & Kuhn, A. (2017) · Immunologic Research · doi:10.1007/s12026-017-8904-4 · Cited by 28 · see citing works

T regulatory cells in B‐cell malignancy – tumour support or kiss of death?

Lindqvist, C. A., & Loskog, A. (2011) · Immunology · doi:10.1111/j.1365-2567.2011.03539.x · Cited by 28 · see citing works

Combination therapy with oral PUVA and corticosteroid for recalcitrant alopecia areata

Ito, T., Aoshima, M., Ito, N., Uchiyama, I., Sakamoto, K., Kawamura, T., Yagi, H., Hashizume, H., & Takigawa, M. (2009) · Archives of Dermatological Research · doi:10.1007/s00403-009-0936-8 · Cited by 28 · see citing works

Good Manufacturing Practice-Compliant Production and Lot-Release of Ex Vivo Expanded Regulatory T Cells As Basis for Treatment of Patients with Autoimmune and Inflammatory Disorders

Wiesinger, M., Stoica, D., Rößner, S., Lorenz, C., Fischer, A., Atreya, R. N., Neufert, C. F., Atreya, I., Scheffold, A., Schuler‐Thurner, B., et al. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01371 · Cited by 26 · see citing works

FOXP3+regulatory T cells as biomarkers in human malignancies

Bignone, P. A., & Banham, A. H. (2008) · Expert Opinion on Biological Therapy · doi:10.1517/14712590802494022 · Cited by 25 · see citing works

Flow cytometry CD4+CD26−CD38+ lymphocyte subset in the microenvironment of Hodgkin lymphoma-affected lymph nodes

Gaetano, R. D., Gasparetto, V., Padoan, A., Callegari, B., Candiotto, L., Sanzari, M. C., Scapinello, A., & Tagariello, G. (2014) · Annals of Hematology · doi:10.1007/s00277-014-2044-x · Cited by 23 · see citing works

Expression of full-length and splice forms of FoxP3 in rheumatoid arthritis

Ryder, L. R., Woetmann, A., Madsen, H. O., Ødum, N., Ryder, L. P., Bliddal, H., Danneskiold‐Samsøe, B., Ribel-Madsen, S. M., & Bartels, E. (2010) · Scandinavian Journal of Rheumatology · doi:10.3109/03009740903555374 · Cited by 22 · see citing works

Isolation, expansion and functional assessment of CD4+CD25+FoxP3+ regulatory T cells and Tr1 cells from uremic patients awaiting kidney transplantation

Berglund, D., Korsgren, O., Lorant, T., Schneider, K., Tufveson, G., & Carlsson, B. (2011) · Transplant Immunology · doi:10.1016/j.trim.2011.09.003 · Cited by 20 · see citing works

Analysis of Human FOXP3+ Treg Cells Phenotype and Function

d'Hennezel, E. B., & Piccirillo, C. A. (2011) · Methods in molecular biology · doi:10.1007/978-1-61737-979-6_13 · Cited by 19 · see citing works

T-cell proliferation and forkhead box P3 expression in human T cells are dependent on T-cell density: physics of a confined space?

Bernardo, D., Al-Hassi, H. O., Mann, E. R., Tee, D., Murugananthan, A., Peake, S. T. C., Hart, A. L., & Knight, S. C. (2012) · Human Immunology · doi:10.1016/j.humimm.2011.12.017 · Cited by 17 · see citing works

Immunophenotyping of Human Regulatory T Cells

Staats, J. S. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9650-6_9 · Cited by 13 · see citing works

Defense of the clone: antibody 259D effectively labels human FOXP3 in a variety of applications

Fox, B. C., Bignone, P. A., Brown, P. J., & Banham, A. H. (2008) · Blood · doi:10.1182/blood-2008-01-134148 · Cited by 11 · see citing works

Determination of Absolute Counts of Circulating Regulatory T Cells in Cynomolgus Macaques Using an Optimized Flow Cytometric Method

Clark, S. M., Narayanan, P. K., & Fort, M. M. (2011) · Toxicologic Pathology · doi:10.1177/0192623311425056 · Cited by 9 · see citing works

Immunological Profiling of Haemodialysis Patients and Young Healthy Individuals with Implications for Clinical Regulatory T Cell Sorting

Bergström, M., Joly, A.-L., Seiron, P., Isringhausen, S., Modig, E., Fellström, B., Andersson, J., & Berglund, D. (2015) · Scandinavian Journal of Immunology · doi:10.1111/sji.12287 · Cited by 6 · see citing works

Transcription Factor Evaluation by Flow Cytometry

Fenoglio, D., & Altosole, T. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1311-5_3 · Cited by 2 · see citing works

Regulatory T Cells in Colorectal Cancer

Jafariniaorcid, M., Lotfiorcid, N., Ganjalikhani-hakmiorcid, M., & Rezaei, A. (2018) · Immunoregulation · doi:10.32598/immunoregulation.1.1.5 · Cited by 1 · see citing works

T Regulatory Cells – Friends or Foes?

Lindqvist, C. A. (2010) · KTH Publication Database DiVA (KTH Royal Institute of Technology)

Regulatory T cells in human pregnancy

Mjösberg, J. (2010) · DiVA (Linkoping University)

How Many Colors to Identify Regulatory T Cells in Humans

Battaglia, A., & Fattorossi, A. (2014)

Assessing the purity of regulatory T cells: A humble reminder

Berglund, E., Sellberg, F., & Berglund, D. (2016) · Cytotherapy · doi:10.1016/j.jcyt.2016.10.012

The influence of regulatory T cells and mobilisation regimens on graft versus malignancy, graft versus host disease and relapse in haematopoietic progenitor cell transplantation

Wells, J. C. (2015) · UWE Research Repository (UWE Bristol)

Correspondence To the editor:

(2016)

Response: Anti–human FOXP3 mAb PCH101 stains activated human naïve T cells nonspecifically

Tran, D. Q., & Shevach, E. M. (2007) · Blood · doi:10.1182/blood-2007-09-113894 · Cited by 10 · see citing works

Association of two clones allows for optimal detection of human

Presicce, P., Moreno-fern, M. E., Lages, C. S., Orsborn, K. I., & Claire, A. (2015)

Association of two clones allows for optimal detection of human FOXP3

Presicce, P., Moreno‐Fernandez, M. E., Lages, C. S., Orsborn, K. I., & Chougnet, C. A. (2010) · Cytometry Part A · doi:10.1002/cyto.a.20875 · Cited by 30 · see citing works

Characterization of stable human CD4+ T cell clones that constitutively express high levels of CD25 and FOXP3.

Stockis, J. (2008) · Digital Access to Libraries (Université catholique de Louvain (UCL), l'Université de Namur (UNamur) and the Université Saint-Louis (USL-B))