The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Biologic properties and enucleation of red blood cells from human embryonic stem cells
Open accessAbstract
Human erythropoiesis is a complex multistep process that involves the differentiation of early erythroid progenitors to mature erythrocytes. Here we show that it is feasible to differentiate and mature human embryonic stem cells (hESCs) into functional oxygen-carrying erythrocytes on a large scale (1010-1011 cells/6-well plate hESCs). We also show for the first time that the oxygen equilibrium curves of the hESC-derived cells are comparable with normal red blood cells and respond to changes in pH and 2,3-diphosphoglyerate. Although these cells mainly expressed fetal and embryonic globins, they also possessed the capacity to express the adult β-globin chain on further maturation in vitro. Polymerase chain reaction and globin chain specific immunofluorescent analysis showed that the cells increased expression of β-globin (from 0% to > 16%) after in vitro culture. Importantly, the cells underwent multiple maturation events, including a progressive decrease in size, increase in glycophorin A expression, and chromatin and nuclear condensation. This process resulted in extrusion of the pycnotic nuclei in up to more than 60% of the cells generating red blood cells with a diameter of approximately 6 to 8 μm. The results show that it is feasible to differentiate and mature hESCs into functional oxygen-carrying erythrocytes on a large scale.
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Cites
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells
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Proof of principle for transfusion of in vitro–generated red blood cells
Giarratana, M.-C., Rouard, H., Dumont, A., Kiger, L., Safeukui, I., Le Pennec, P.-Y., François, S., Trugnan, G., Peyrard, T., Marie, T., et al. (2011) · Blood · doi:10.1182/blood-2011-06-362038 · Cited by 342
Inhibition of pluripotent stem cell-derived teratoma formation by small molecules
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Renella, R., Roberts, N. A., Brown, J. M., De Gobbi, M., Bird, L. E., Hassanali, T., Sharpe, J. A., Sloane-Stanley, J., Ferguson, D. J. P., Cordell, J., et al. (2011) · Blood · doi:10.1182/blood-2010-09-308478 · Cited by 54
Metadata
- Authors
- Lu, S.-J., Feng, Q., Park, J. S., Vida, L., Lee, B.-S., Strausbauch, M., Wettstein, P. J., Honig, G. R., & Lanza, R.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2008-12-01
- Volume
- 112
- Issue
- 12
- Pages
- 4475-4484
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2140717627
- Cited by
- 293
- References
- 49
- FWCI
- 11.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Lu, S.-J., Feng, Q., Park, J. S., Vida, L., Lee, B.-S., Strausbauch, M., Wettstein, P. J., Honig, G. R., & Lanza, R. (2008). Biologic properties and enucleation of red blood cells from human embryonic stem cells. Blood. https://doi.org/10.1182/blood-2008-05-157198
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