The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation
Open accessAbstract
Neutrophil research relies largely on studies with highly purified cells. Yet the isolation procedures induce changes in surface expression of several proteins. We used a large panel of monoclonal antibodies (MoAbs) to characterize in detail the phenotypic changes during isolation and stimulation of human neutrophils. Centrifugation on density gradients appears to be the crucial step that causes an increase in expression of antigens not detectable on neutrophils in whole blood samples (cytochrome b558 recognized by MoAb 7D5; and CD10) or expressed at significantly lower levels (CD11a, CD11b, CD11c, CD13, CD16, CD45, and CD67). Other antigens were unaffected by the density gradient centrifugation step (CD32, CD54, CD58, Leu-8, HLA class I). Upregulation of antigens was also determined by stimulation of purified neutrophils. Upregulation of CD63 was an excellent marker for release from azurophil granules. We subsequently related the surface antigen expression to functional activities of purified neutrophils. From these experiments, we concluded that 7D5-as “early activation” marker--does not necessarily discriminate between primed or resting neutrophils with respect to NADPH oxidase activity.
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Citations
Cites
Tissue Destruction by Neutrophils
Epstein, F. H., & Weiss, S. J. (1989) · New England Journal of Medicine · doi:10.1056/nejm198902093200606 · Cited by 3,950
Parkos, C. A., Allen, R. A., Cochrane, C. G., & Jesaitis, A. J. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113128 · Cited by 455
Two cytosolic neutrophil oxidase components absent in autosomal chronic granulomatous disease
Volpp, B. D., Nauseef, W. M., & Clark, R. A. (1988) · Science · doi:10.1126/science.2848318 · Cited by 407
Cited by
Neutrophil-Derived Proteins: Selling Cytokines by the Pound
Cassatella, M. A. (1999) · Advances in Immunology · doi:10.1016/s0065-2776(08)60791-9 · Cited by 560
Human neutrophil granules and secretory vesicles
Borregaard, N., Lollike, K., Kjeldsen, L., Sengeløv, H., Bastholm, L., Nielsen, M. H., & Bainton, D. F. (1993) · European Journal Of Haematology · doi:10.1111/j.1600-0609.1993.tb00629.x · Cited by 295
Activated neutrophils express proteinase 3 on their plasma membrane in vitro and in vivo
CSERNOK, E., ERNST, M., SCHMITT, W., BAINTON, D. F., & GROSS, W. L. (2008) · Clinical and Experimental Immunology · doi:10.1111/j.1365-2249.1994.tb06518.x · Cited by 289
(1995) · PubMed · Cited by 215
Distinct Oral Neutrophil Subsets Define Health and Periodontal Disease States
Fine, N., Hassanpour, S., Borenstein, A., Sima, C., Oveisi, M., Scholey, J., Cherney, D., & Glogauer, M. (2016) · Journal of Dental Research · doi:10.1177/0022034516645564 · Cited by 163
Metadata
- Authors
- Kuijpers, T., Tool, A., van der Schoot, C., Ginsel, L., Onderwater, J., Roos, D., & Verhoeven, A.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1991-08-15
- Volume
- 78
- Issue
- 4
- Pages
- 1105-1111
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W179223508
- Cited by
- 452
- References
- 3
- FWCI
- 7.05
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. https://app.cochat.ai/papers/10.1182/blood.v78.4.1105.1105
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- Kuijpers, T., Tool, A., van der Schoot, C., Ginsel, L., Onderwater, J., Roos, D., & Verhoeven, A. (1991). Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood. https://doi.org/10.1182/blood.v78.4.1105.1105
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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